Chimeric self-sufficient P450cam-RhFRed biocatalysts with broad substrate scope

Scholarly Works

• Jones, B. S.; Ross, C. M.; Foley, G.; Pozhydaieva, N.; Sharratt, J. W.; Kress, N.; Seibt, L. S.; Thomson, R. E. S.; Gumulya, Y.; Hayes, M. A.; Gillam, E. M. J.; Flitsch, S. L. Engineering Biocatalysts for the C−H Activation of Fatty Acids by Ancestral Sequence Reconstruction**. Angewandte Chemie 2024, 136. doi:10.1002/ange.202314869
• Jones, B. S.; Ross, C. M.; Foley, G.; Pozhydaieva, N.; Sharratt, J. W.; Kress, N.; Seibt, L. S.; Thomson, R. E. S.; Gumulya, Y.; Hayes, M. A.; Gillam, E. M. J.; Flitsch, S. L. Engineering Biocatalysts for the C-H Activation of Fatty Acids by Ancestral Sequence Reconstruction. Angewandte Chemie (International ed. in English) 2024, 63, e202314869. doi:10.1002/anie.202314869
• Sahil, M.; Singh, T.; Ghosh, S.; Mondal, J. 3site Multisubstrate-Bound State of Cytochrome P450cam. Journal of the American Chemical Society 2023, 145, 23488–23502. doi:10.1021/jacs.3c06144
• Charlton, S. N.; Hayes, M. A. Oxygenating Biocatalysts for Hydroxyl Functionalisation in Drug Discovery and Development. ChemMedChem 2022, 17, e202200115. doi:10.1002/cmdc.202200115
• Wang, F.; Frankowski, K. J. Divergent Electrochemical Carboamidation of Cyclic Amines. The Journal of organic chemistry 2022, 87, 1173–1193. doi:10.1021/acs.joc.1c02534
• Correddu, D.; Di Nardo, G.; Gilardi, G. Self-Sufficient Class VII Cytochromes P450: From Full-Length Structure to Synthetic Biology Applications. Trends in biotechnology 2021, 39, 1184–1207. doi:10.1016/j.tibtech.2021.01.011
• Aranda, C.; Carro, J.; González-Benjumea, A.; Babot, E. D.; Olmedo, A.; Linde, D.; Martínez, Á. T.; Gutiérrez, A. Advances in enzymatic oxyfunctionalization of aliphatic compounds. Biotechnology advances 2021, 51, 107703. doi:10.1016/j.biotechadv.2021.107703
• Finnigan, J.; Young, C.; Cook, D. J.; Charnock, S. J.; Black, G. W. Cytochromes P450 (P450s): A review of the class system with a focus on prokaryotic P450s. Advances in protein chemistry and structural biology 2020, 122, 289–320. doi:10.1016/bs.apcsb.2020.06.005
• Oeschger, R. J.; Su, B.; Yu, I.; Ehinger, C.; Romero, E. A.; He, S.; Hartwig, J. F. Diverse functionalization of strong alkyl C-H bonds by undirected borylation. Science (New York, N.Y.) 2020, 368, 736–741. doi:10.1126/science.aba6146
• Li, Z.; Jiang, Y.; Guengerich, F. P.; Ma, L.; Li, S.; Zhang, W. Engineering cytochrome P450 enzyme systems for biomedical and biotechnological applications. The Journal of biological chemistry 2019, 295, 833–849. doi:10.1074/jbc.rev119.008758
• Xu, L.-H.; Du, Y.-L. Rational and semi-rational engineering of cytochrome P450s for biotechnological applications. Synthetic and systems biotechnology 2018, 3, 283–290. doi:10.1016/j.synbio.2018.10.001
• Zhang, W.; Du, L.; Li, F.; Zhang, X.; Qu, Z.; Han, L.; Li, Z.; Sun, J.; Qi, F.; Yao, Q.; Sun, Y.; Geng, C.; Li, S. Mechanistic Insights into Interactions between Bacterial Class I P450 Enzymes and Redox Partners. ACS Catalysis 2018, 8, 9992–10003. doi:10.1021/acscatal.8b02913
• Andorfer, M. C.; Belsare, K. D.; Girlich, A. M.; Lewis, J. C. Aromatic Halogenation by Using Bifunctional Flavin Reductase-Halogenase Fusion Enzymes. Chembiochem : a European journal of chemical biology 2017, 18, 2099–2103. doi:10.1002/cbic.201700391
• Talmann, L.; Wiesner, J.; Vilcinskas, A. Strategies for the construction of insect P450 fusion enzymes. Zeitschrift fur Naturforschung. C, Journal of biosciences 2017, 72, 405–415. doi:10.1515/znc-2017-0041
• Tavanti, M.; Parmeggiani, F.; Castellanos, J. R. G.; Mattevi, A.; Turner, N. J. One-pot Biocatalytic Double Oxidation of α-Isophorone for the Synthesis of Ketoisophorone. ChemCatChem 2017, 9, 3338–3348. doi:10.1002/cctc.201700620
• Schrittwieser, J. H.; Velikogne, S.; Hall, M.; Kroutil, W. Artificial Biocatalytic Linear Cascades for Preparation of Organic Molecules. Chemical reviews 2017, 118, 270–348. doi:10.1021/acs.chemrev.7b00033
• Ciaramella, A.; Minerdi, D.; Gilardi, G. Catalytically self-sufficient cytochromes P450 for green production of fine chemicals. Rendiconti Lincei 2016, 28, 169–181. doi:10.1007/s12210-016-0581-z
• Cook, D. J.; Finnigan, J.; Cook, K.; Black, G. W.; Charnock, S. J. Cytochromes P450: History, Classes, Catalytic Mechanism, and Industrial Application. Advances in protein chemistry and structural biology 2016, 105, 105–126. doi:10.1016/bs.apcsb.2016.07.003
• Hoffmann, S. M.; Weissenborn, M. J.; Gricman, Ł.; Notonier, S.; Pleiss, J.; Hauer, B. The Impact of Linker Length on P450 Fusion Constructs: Activity, Stability and Coupling. ChemCatChem 2016, 8, 1591–1597. doi:10.1002/cctc.201501397
• Eichler, A.; Gricman, Ł.; Herter, S.; Kelly, P. P.; Turner, N. J.; Pleiss, J.; Flitsch, S. L. Enantioselective Benzylic Hydroxylation Catalysed by P450 Monooxygenases: Characterisation of a P450cam Mutant Library and Molecular Modelling. Chembiochem : a European journal of chemical biology 2016, 17, 426–432. doi:10.1002/cbic.201500536

Explore citations to this article at