Quantification of N-acetylcysteamine activated methylmalonate incorporation into polyketide biosynthesis

• Stephan Klopries,
• Uschi Sundermann and
• Frank Schulz

Beilstein J. Org. Chem. 2013, 9, 664–674, doi:10.3762/bjoc.9.75

• feeding buffer (see below) in an ice bath to reach a final concentration of 0.1 M of the product. DCM was evaporated at room temperature and the remaining white slurry was transferred to 50 mL polypropylene tubes. After centrifugation for 4 min to remove TiO2, the resulting clear solution was sterile

• into polypropylene tubes and centrifuged, and 1 mL of the supernatant was filtered and used for analysis. The most intensively studied PKS, deoxyerythronolide B synthase (DEBS), which catalyzes the key steps in the biosynthesis of the antibiotic erythromycin. DEBS catalyzes the extension of a

Multivalent display of the antimicrobial peptides BP100 and BP143

• Imma Güell,
• Rafael Ferre,
• Kasper K. Sørensen,
• Esther Badosa,
• Iteng Ng-Choi,
• Emilio Montesinos,
• Eduard Bardají,
• Lidia Feliu,
• Knud J. Jensen and
• Marta Planas

Beilstein J. Org. Chem. 2012, 8, 2106–2117, doi:10.3762/bjoc.8.237

• carried out on a fully automated Syro II peptide synthesizer (MultiSynTech). Manual peptide synthesis was performed in polypropylene syringes fitted with a polyethylene porous disk. Solvents and soluble reagents were removed by suction. Chemicals were purchased from commercial suppliers Sigma–Aldrich

Modulating the activity of short arginine-tryptophan containing antibacterial peptides with N-terminal metallocenoyl groups

• H. Bauke Albada,
• Alina-Iulia Chiriac,
• Michaela Wenzel,
• Maya Penkova,
• Julia E. Bandow,
• Hans-Georg Sahl and
• Nils Metzler-Nolte

Beilstein J. Org. Chem. 2012, 8, 1753–1764, doi:10.3762/bjoc.8.200

• Table 3) Determination of MIC values was performed in 96-well polypropylene microtiter plates (Life Technologies) in order to reduce the AMP binding [66]. A series of 2-fold dilutions of the peptides was prepared directly in the plate in half-concentrated MH broth. The tested strains were grown to an

Miniemulsion polymerization as a versatile tool for the synthesis of functionalized polymers

• Daniel Crespy and
• Katharina Landfester

Beilstein J. Org. Chem. 2010, 6, 1132–1148, doi:10.3762/bjoc.6.130

• that 92% of the surfactant remained grafted after dialysis. Nanoparticles and nanocapsules from polymerization of styrene and/or divinylbenzene in miniemulsion could be produced in the presence of a polymerizable derivative of polyethylene oxide/polypropylene oxide which was used to stabilize the

• require very high polymerization temperatures. Star copolymers of polyethylene glycol and polypropylene glycol were crosslinked in inverse miniemulsion via an esterification reaction with a dithiodicarboxylic acid to yield nanogels [124]. The disulfide bonds were subsequently cleaved by reduction to yield