An easily accessible sulfated saccharide mimetic inhibits in vitro human tumor cell adhesion and angiogenesis of vascular endothelial cells

• Grazia Marano,
• Claas Gronewold,
• Martin Frank,
• Anette Merling,
• Christian Kliem,
• Sandra Sauer,
• Manfred Wiessler,
• Eva Frei and
• Reinhard Schwartz-Albiez

Beilstein J. Org. Chem. 2012, 8, 787–803, doi:10.3762/bjoc.8.89

• by Lowry’s method) were separated under nonreducing conditions in a 7.5% sodium dodecyl sulfate-polyacrylamide gel (SDS-PAGE) polymerized together with 0.1% gelatin. After electrophoresis, gels were washed twice in 2.5% Triton X-100 and four times in H2O before overnight incubation in gelatinase

Mutational analysis of a phenazine biosynthetic gene cluster in Streptomyces anulatus 9663

• Orwah Saleh,
• Katrin Flinspach,
• Lucia Westrich,
• Andreas Kulik,
• Bertolt Gust,
• Hans-Peter Fiedler and
• Lutz Heide

Beilstein J. Org. Chem. 2012, 8, 501–513, doi:10.3762/bjoc.8.57

• selection of recombinant strains. Chemicals Kanamycin and carbenicillin were purchased from Genaxxon BioSciences GmbH (Biberach, Germany) and phenazine 1-carboxylic acid was from InFormatik. IPTG, Tris, NaCl, glycerol, dithiothreitol, MgCl2, formic acid, sodium dodecyl sulfate, polyacrylamide, and EDTA were

Surfactant catalyzed convenient and greener synthesis of tetrahydrobenzo[a]xanthene-11-ones at ambient temperature

• Pravin V. Shinde,
• Amol H. Kategaonkar,
• Bapurao B. Shingate and
• Murlidhar S. Shingare

Beilstein J. Org. Chem. 2011, 7, 53–58, doi:10.3762/bjoc.7.9

• cetyltrimethylammonium bromide (CTAB), methyltriphenylphosphonium bromide (MTPPB) and cetylpyridinium chloride (CPC) as well as an anionic surfactant, sodium dodecyl sulfate (SDS), were utilized at ambient temperature. From these preliminary studies, it was observed that the anionic surfactant SDS and cationic

Cross-metathesis of allylcarboranes with O-allylcyclodextrins

• Ivan Šnajdr,
• Zbyněk Janoušek,
• Jindřich Jindřich and
• Martin Kotora

Beilstein J. Org. Chem. 2010, 6, 1099–1105, doi:10.3762/bjoc.6.126

• , presumably because of their insolubility in the aforementioned solvents. To overcome the problem of the solubility of free O-allylcyclodextrins, the reaction was carried out in water in the presence of surfactant (SDS – sodium dodecyl sulfate), however, cross-metathesis did not occur. Conclusion The results

Phaeochromycins F–H, three new polyketide metabolites from Streptomyces sp. DSS-18

• Jian Li,
• Chun-Hua Lu,
• Bao-Bing Zhao,
• Zhong-Hui Zheng and
• Yue-Mao Shen

Beilstein J. Org. Chem. 2008, 4, No. 46, doi:10.3762/bjoc.4.46

• was obtained by sodium dodecyl sulfate (SDS)-proteinase K lysis, selective precipitation of cell debris and polysaccharides with CTAB (hexadecyltrimethylammonium bromide), and isopropanol precipitation [9]. The bacterial strain was incubated on slope of GS media in a test tube at 28 °C for 5 d to