Towards practical biocatalytic Baeyer- Villiger reactions: applying a thermostable enzyme in the gram- scale synthesis of optically- active lactones in a two-liquid- phase system

• Frank Schulz,
• François Leca,
• Frank Hollmann and
• Manfred T. Reetz

Beilstein J. Org. Chem. 2005, 1, No. 10, doi:10.1186/1860-5397-1-10

• stability of the enzyme resulted from the use of detergents. Overall we found a buffer consisting of 50 mM Tris-HCl (pH 8.0 at 40°C), 2 g/L BSA, 5% (w/v) glucose, 5% (w/v) lactose, and 0.1% (v/v) Tween-20 to be optimal in maintaining PAMO stability. The influence of different solvents was then assayed in a