New amphiphilic glycopolymers by click functionalization of random copolymers – application to the colloidal stabilisation of polymer nanoparticles and their interaction with concanavalin A lectin

• Otman Otman,
• Paul Boullanger,
• Eric Drockenmuller and
• Thierry Hamaide

Beilstein J. Org. Chem. 2010, 6, No. 58, doi:10.3762/bjoc.6.58

• -22), 66.93 (C-14), 62.05 (C-24), 58.07 (C-10), 50.65 (C-13), 48.40 (C-1), 40.04 (C-7), 35.00 (C-2), 31.48 (C-4), 26.00–26.50 (C-6, C-8), 18.27 (C-3). Nanoparticle formation Ethyl acetate and water were contacted for 2 h in order to obtain mutually saturated solutions. Polycaprolactone (80000 g/mol

• emulsified by sonication (ultrasonic processor Vibra Cell VCX-750) for 2 min at 525 W. De-ionized water (175 mL) was then added while stirring the solution at 700–800 rpm. The nanoparticle suspension was then concentrated at atmospheric pressure to 30–35 mL by stirring and heating to 70–80 °C. The sizes of

• containing D-mannose with con A In a 20 mL flask, 8.7 mg of concanavalin A was dissolved without stirring in 10 mL of phosphate-buffered saline (PBS, pH = 6.8, 0.1 M) over 16 hours. The flask was then stored at 4 °C. The nanoparticle stock solution (200 mg of dry matter in 3.5 mL H2O) was diluted in PBS and