Multivalent display of the antimicrobial peptides BP100 and BP143

• Imma Güell,
• Rafael Ferre,
• Kasper K. Sørensen,
• Esther Badosa,
• Iteng Ng-Choi,
• Emilio Montesinos,
• Eduard Bardají,
• Lidia Feliu,
• Knud J. Jensen and
• Marta Planas

Beilstein J. Org. Chem. 2012, 8, 2106–2117, doi:10.3762/bjoc.8.237

• was obtained. It has been previously reported that silanes under acidic conditions can promote the reduction of aldehydes [27]. Cleavage of the peptidyl resins with TFA/H2O (95:5) followed by purification by reversed-phase high-performance liquid chromatography (RP-HPLC) afforded peptide aldehydes 4

The use of glycoinformatics in glycochemistry

• Thomas Lütteke

Beilstein J. Org. Chem. 2012, 8, 915–929, doi:10.3762/bjoc.8.104

• data with a variety of tools and information on other biomolecules. The KEGG portal has a particular focus on biosynthetic pathways. EUROCarbDB was developed to store primary data of mass spectrometry (MS), NMR and high performance liquid chromatography (HPLC) experiments. In addition to data imported

Triterpenoid saponins from the roots of Acanthophyllum gypsophiloides Regel

• Elena A. Khatuntseva,
• Vladimir M. Men’shov,
• Alexander S. Shashkov,
• Yury E. Tsvetkov,
• Rodion N. Stepanenko,
• Raymonda Ya. Vlasenko,
• Elvira E. Shults,
• Genrikh A. Tolstikov,
• Tatjana G. Tolstikova,
• Dimitri S. Baev,
• Vasiliy A. Kaledin,
• Nelli A. Popova,
• Valeriy P. Nikolin,
• Pavel P. Laktionov,
• Anna V. Cherepanova,
• Tatiana V. Kulakovskaya,
• Ekaterina V. Kulakovskaya and
• Nikolay E. Nifantiev

Beilstein J. Org. Chem. 2012, 8, 763–775, doi:10.3762/bjoc.8.87

• solution (Fluka). A syringe injection was used for solutions in acetonitrile, methanol, or water (flow rate 3 μL/min). Nitrogen was applied as a dry gas; the interface temperature was set to 180 °C. High-performance liquid chromatography (HPLC) was carried out on a C18 reversed-phase column (Ascentis C18

Cyanoethylation of the glucans dextran and pullulan: Substitution pattern and formation of nanostructures and entrapment of magnetic nanoparticles

• Kathrin Fiege,
• Heinrich Lünsdorf,
• Sevil Atarijabarzadeh and
• Petra Mischnick

Beilstein J. Org. Chem. 2012, 8, 551–566, doi:10.3762/bjoc.8.63

• infrared spectroscopy (ATR–IR). Gas–liquid chromatography (GLC) in combination with mass spectrometry (MS) was employed for the analysis of glucose derivatives after depolymerization of the cyanoethylglucans. The DS of heterogenic atoms containing polysaccharide derivatives can be followed by elementary

• residual solvent signals. ATR–IR spectra were recorded by using a Bruker Tensor 27 attenuated total reflectance infrared (ATR–IR) spectrometer. Elementary analysis (EA) was performed on a Thermoquest EA 1112 analyser. The data given is always the average of two measurements. Gas–liquid chromatography (GLC

Mutational analysis of a phenazine biosynthetic gene cluster in Streptomyces anulatus 9663

• Orwah Saleh,
• Katrin Flinspach,
• Lucia Westrich,
• Andreas Kulik,
• Bertolt Gust,
• Hans-Peter Fiedler and
• Lutz Heide

Beilstein J. Org. Chem. 2012, 8, 501–513, doi:10.3762/bjoc.8.57

• concentrated in vacuo to an aqueous residue, adjusted to pH 4.0 by using HCl and extracted with ethyl acetate. The ethyl acetate extract was first treated with petrol ether. After evaporation, the extract residue was fractioned by using a liquid chromatography system with a Sephadex LH20 column (2.5 × 90 cm

Conserved and species-specific oxylipin pathways in the wound-activated chemical defense of the noninvasive red alga Gracilaria chilensis and the invasive Gracilaria vermiculophylla

• Martin Rempt,
• Florian Weinberger,
• Katharina Grosser and
• Georg Pohnert

Beilstein J. Org. Chem. 2012, 8, 283–289, doi:10.3762/bjoc.8.30

• measurements were performed on an UPLC–MS system equipped with a 2996 PDA detector and a Q-tof micro ESIMS (Waters, Manchester, UK). For separation of the analytes, a BEH C18 column (2.1 × 50 mm, particle size 1.7 µm) was used. LC on a semipreparative column was carried out by using a LC-8A liquid

• chromatography system from Shimadzu (Duisburg, Germany) equipped with a SPD-10AV UV–vis detector. A Licro Chart® 250-10 Purosphere® RP-18 endcapped column (particle size 5µm) supplied by Merck (Darmstadt, Germany) was used for separation. HPLC separation on an analytical scale was performed on the same LC system

Marilones A–C, phthalides from the sponge-derived fungus Stachylidium sp.

• Celso Almeida,
• Stefan Kehraus,
• Miguel Prudêncio and
• Gabriele M. König

Beilstein J. Org. Chem. 2011, 7, 1636–1642, doi:10.3762/bjoc.7.192

• , 70–230 mesh) was used for vacuum liquid chromatography (VLC). Columns were wet-packed under vacuum with petroleum ether (PE). Before applying the sample solution, the columns were equilibrated with the first designated eluent. Standard columns for crude extract fractionation had dimensions of 13 × 4

Asymmetric Au-catalyzed cycloisomerization of 1,6-enynes: An entry to bicyclo[4.1.0]heptene

• Alexandre Pradal,
• Chung-Meng Chao,
• Patrick Y. Toullec and
• Véronique Michelet

Beilstein J. Org. Chem. 2011, 7, 1021–1029, doi:10.3762/bjoc.7.116

• were recorded on a Bruker AV 300 instrument. All signals were expressed as ppm (δ) and internally referenced to residual proton solvent signals. Coupling constants (J) are reported in Hz and refer to apparent peak multiplicities. Enantiomeric excesses were determined by high pressure liquid

• chromatography analyses (HPLC) on Waters instruments (Waters 486 detector, 717 autosampler equipped with Daicel Chiralcel OD-H, OJ and Chiralpak IA, AD, λ = 215 nm). Optical rotation measurements were conducted on a Perkin–Elmer 241 polarimeter at 589 nm. Enynes 5 [71], 2a [72], 2b–e [46], 1a [73], 1b [74], 1c,d

Continuous flow hydrogenation using polysilane-supported palladium/alumina hybrid catalysts

• Hidekazu Oyamada,
• Takeshi Naito and
• Shū Kobayashi

Beilstein J. Org. Chem. 2011, 7, 735–739, doi:10.3762/bjoc.7.83

• continuous flow reactor and an image of the top of the column are shown in Figure 1. A high performance liquid chromatography (HPLC) pump was used to feed the substrate into the central hole in the top of the column, which was filled with the Pd/(PSi–Al2O3) catalyst. Hydrogen gas was introduced into the six

Towards racemizable chiral organogelators

• Jian Bin Lin,
• Debarshi Dasgupta,
• Seda Cantekin and
• Albertus P. H. J. Schenning

Beilstein J. Org. Chem. 2010, 6, 960–965, doi:10.3762/bjoc.6.107

• hydrogen-bond interactions between the peptide head groups of neighbouring organogelator molecules. The enantiomerically pure organogelator can be racemized by the base DBU (1,8-diazabicyclo[5.4.0]undec-7-ene) as was evident from chiral high-performance liquid chromatography analysis. Keywords: chirality

• -didodecyloxybenzaldehyde [19] led to compound 3 in 50% yield (Scheme 1). Both R-3 and S-3 were purified by recrystallization and fully characterized. Chiral high-performance liquid chromatography (HPLC) showed an ee of more than 99% for the enantiomers. Interestingly, 1H NMR spectroscopy in chloroform revealed that the

Preparation and NMR spectra of four isomeric diformyl[2.2]paracyclophanes (cyclophanes 66)

• Ina Dix,
• Henning Hopf,
• Thota B. N. Satyanarayana and
• Ludger Ernst

Beilstein J. Org. Chem. 2010, 6, 932–937, doi:10.3762/bjoc.6.104

• beginning to attract the attention of other research groups [6][7][10][11][12][13]. Results and Discussion Separation of the diformyl[2.2]paracyclophanes 4 We have separated the isomer mixture 4 by different methods. The easiest way is by middle pressure liquid chromatography (MPLC), which readily affords