Beilstein J. Org. Chem.2024,20, 2242–2253, doi:10.3762/bjoc.20.192
tested additives.
Keywords: cell-free protein synthesis; cGAS; Escherichiacolicell-freeextract; sfGFP; TX-TL; Introduction
In addition to other applications such as biomanufacturing or biosensing, cell-free protein synthesis (CFPS) of enzymes has established itself as a tool for rapid screening of
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Graphical Abstract
Figure 1:
CFPS of sfGFP with different technical additives at various concentrations. Experiments with 2% PEG...