Cell-free protein synthesis with technical additives – expanding the parameter space of in vitro gene expression

• Tabea Bartsch,
• Stephan Lütz and
• Katrin Rosenthal

Beilstein J. Org. Chem. 2024, 20, 2242–2253, doi:10.3762/bjoc.20.192

• tested additives. Keywords: cell-free protein synthesis; cGAS; Escherichia coli cell-free extract; sfGFP; TX-TL; Introduction In addition to other applications such as biomanufacturing or biosensing, cell-free protein synthesis (CFPS) of enzymes has established itself as a tool for rapid screening of

• the production of non-ribosomal peptide synthetases with a molecular weight higher than 100 kDa [57]. However, these enzymes are often of great interest for specific applications in biomanufacturing. Cyclic GMP-AMP synthase (cGAS) is one of these enzymes. cGAS and its biocatalytic product 2’3’-cyclic

• GMP-AMP (cGAMP) are part of the innate immune response in higher eukaryotes [58]. cGAMP is therefore a promising candidate for pharmaceutical applications [59]. The successful synthesis of the fusion protein of truncated human cGAS and superfolder GFP (thscGAS-sfGFP) using the in-house E. coli-based