Cell-free protein synthesis with technical additives – expanding the parameter space of in vitro gene expression

• Tabea Bartsch,
• Stephan Lütz and
• Katrin Rosenthal

Beilstein J. Org. Chem. 2024, 20, 2242–2253, doi:10.3762/bjoc.20.192

• influence on the synthesis performance of CFPS. Polymers, DES, and organic solvents were considered to modify the fluid properties. Polymers and deep eutectic solvents (DES) as additives in CFPS Polymers (PEG, methylcellulose (MC), and carboxymethylcellulose (CMC)) and DES (choline chloride/urea, betaine

• carboxymethylcellulose (CMC) cover a wide range of viscosities including that of the cytoplasm up to a very viscous solution. The concentrations of methylcellulose and carboxymethylcellulose are limited to 2% as the viscosity would have become too high. Simultaneously, polymers contribute to the concentration of

• translation. While transcription is stable up to concentrations of 10% PEG-8000, translation is already inhibited at a concentration of 1% [48]. When the viscosities are increased with methylcellulose or carboxymethylcellulose, a less negative influence on the sfGFP synthesis is observed. This indicates that

Cage-like microstructures via sequential Ugi reactions in aqueous emulsions

• Rita S. Alqubelat,
• Yaroslava A. Menzorova and
• Maxim A. Mironov

Beilstein J. Org. Chem. 2024, 20, 2078–2083, doi:10.3762/bjoc.20.179

• . At the first stage, submicron colloidal particles based on carboxymethylcellulose and chitosan with a domain structure were obtained in an aqueous suspension. In the second stage, the Ugi reaction was carried out on the surface of the Pickering emulsions with toluene. Removal of toluene and

• redissolution in water resulted in colloidosomes with large holes on the surface. Varying the cross-link density during the Ugi reaction made it possible to obtain structures with different hole sizes. Keywords: carboxymethylcellulose; chitosan; colloidosomes; Pickering emulsions; Ugi reaction; Introduction

• droplets. Fixing this structure with a cross-linker opened the way to the one-step synthesis of colloidosomes with large pores on the surface. Results and Discussion Previously, we have obtained stable colloidal particles by ionic gelation of carboxymethylcellulose (CMC) and low-molecular-weight chitosan

A complete series of 6-deoxy-monosubstituted tetraalkylammonium derivatives of α-, β-, and γ-cyclodextrin with 1, 2, and 3 permanent positive charges

• Martin Popr,
• Simona Hybelbauerová and
• Jindřich Jindřich

Beilstein J. Org. Chem. 2014, 10, 1390–1396, doi:10.3762/bjoc.10.142

• decomposition of DMF or by the formylation side reactions. The authors recovered pure 4 after lengthy chromatographic separation on a carboxymethylcellulose column, involving the collection of 150 fractions. We decided to optimize the reaction conditions with the goal of improving the yield and avoiding the