Beilstein J. Org. Chem.2013,9, 966–973, doi:10.3762/bjoc.9.111
, clickligation of a fluorescent dye, and gel electrophoresis revealed specific labeling of a single 24 kDa band that could be blocked with an active competitor. Future work will focus on identifying the labeled protein(s).
Keywords: CCG-1423; clickligation; photoaffinity labeling; Rho pathway
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Graphical Abstract
Figure 1:
Structures of lead Rho/MKL1/SRF inhibitor 1 and conformationally restricted analogue 2.
Beilstein J. Org. Chem.2010,6, No. 13, doi:10.3762/bjoc.6.13
present in biopolymers and react selectively with each other in aqueous solutions.
The “click” ligation can avoid the time-consuming synthesis of phosphoramidites as DNA building blocks which is especially important for brightly emitting fluorophores that are not compatible with the acidic, oxidative or
basic conditions of automated DNA phosphoramidite chemistry and/or DNA workup. We recently presented the postsynthetic incorporation of Nile Blue and a coumarin dye as representatives of base-labile fluorophores by the “click” ligation strategy [17]. Several other fluorophores (spanning the whole
-threoninol [38] and (S)-[17] and (R)-3-amino-1,2-propanediol linker of this study (to the right).
Synthesis of the R-configured DNA building block 3 and postsynthetic clickligation of the Nile Blue-modified single strands (ss) DNA1 and DNA2 that form the double strands (ds) DNA1Y and DNA2Y with the
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Graphical Abstract
Scheme 1:
Chirality of C-3 of natural 2′-deoxyribofuranosides (left) in comparison with the acyclic D-threoni...