Improved deconvolution of natural products’ protein targets using diagnostic ions from chemical proteomics linkers

• Andreas Wiest and
• Pavel Kielkowski

Beilstein J. Org. Chem. 2024, 20, 2323–2341, doi:10.3762/bjoc.20.199

• discuss the properties and applications of different chemical proteomics linkers with special focus on their fragmentation releasing diagnostic ions and how these may improve the confidence in identified active compound–peptide conjugates. The application of advanced search options improves the

• identification rates and may help to identify otherwise difficult to find interactions between active compounds and proteins, which may result from unperturbed conditions, and thus are of high physiological relevance. Keywords: chemical proteomics; diagnostic ions; mass spectrometry; target identification

• fragmentation performance, the combination of HCD and ETD can be used in a single MS measurement. In such methods ETD is only triggered by probe-specific diagnostic ions released by the faster HCD fragmentation with the aim to fragment the same parent ion again with ETD to obtain a better fragmentation spectrum

Identification, synthesis and mass spectrometry of a macrolide from the African reed frog Hyperolius cinnamomeoventris

• Markus Menke,
• Pardha Saradhi Peram,
• Iris Starnberger,
• Walter Hödl,
• Gregory F.M. Jongsma,
• David C. Blackburn,
• Mark-Oliver Rödel,
• Miguel Vences and
• Stefan Schulz

Beilstein J. Org. Chem. 2016, 12, 2731–2738, doi:10.3762/bjoc.12.269

• spectrometry. Proposed mass spectrometric fragmentation of macrolides 1 and 2 leading to diagnostic ions of the ion series CnH2n−4O2 (Cn+4H2n+4O2 in the figure), indicating the position of the double bond in the ring. Synthesis of (9Z,13R)-tetradec-9-en-13-olide (2). Synthesis of (5Z,13R)-tetradec-5-en-13