New variochelins from soil-isolated Variovorax sp. H002

• Jabal Rahmat Haedar,
• Aya Yoshimura and
• Toshiyuki Wakimoto

Beilstein J. Org. Chem. 2024, 20, 692–700, doi:10.3762/bjoc.20.63

• filter, and then injected into an LC–MS system, Cosmosil 5C18-MS-II (2 mm ID × 100 mm, Nacalai Tesque) (oven 40 °C; flowrate 0.2 mL/min) using a gradient of MeCN/H2O (1:9 to 6:4) containing 0.1% formic acid for 45 minutes. The standards were processed in the same manner. Whole genome analysis of

Chemical and biosynthetic potential of Penicillium shentong XL-F41

• Ran Zou,
• Xin Li,
• Xiaochen Chen,
• Yue-Wei Guo and
• Baofu Xu

Beilstein J. Org. Chem. 2024, 20, 597–606, doi:10.3762/bjoc.20.52

• activity against Candia albicans. Moreover, genome sequencing of Penicillium shentong XL-F41 uncovered abundant silent biosynthetic gene clusters, indicating the need for future efforts to activate these clusters and unlock the full chemical potential of the fungus. Keywords: genome analysis; indole

Intermediates and shunt products of massiliachelin biosynthesis in Massilia sp. NR 4-1

• Till Steinmetz,
• Blaise Kimbadi Lombe and
• Markus Nett

Beilstein J. Org. Chem. 2023, 19, 909–917, doi:10.3762/bjoc.19.69

• order to scavenge iron from the environment. An example is the thiazoline-containing natural product massiliachelin, which is produced by Massilia sp. NR 4-1 under iron-deficient conditions. Based on experimental evidence and genome analysis, it was suspected that this bacterium synthesizes further iron

Navigating and expanding the roadmap of natural product genome mining tools

• Friederike Biermann,
• Sebastian L. Wenski and
• Eric J. N. Helfrich

Beilstein J. Org. Chem. 2022, 18, 1656–1671, doi:10.3762/bjoc.18.178

• trained on a set of known precursor genes to detect RiPP precursor genes semi-independently of their subclass. Subsequently, a pan-genome analysis is performed to identify the corresponding BGCs with the putative RiPP precursor genes as seeds. Putative NP BGCs are identified that are organized in operon

A new glance at the chemosphere of macroalgal–bacterial interactions: In situ profiling of metabolites in symbiosis by mass spectrometry

• Marine Vallet,
• Filip Kaftan,
• Veit Grabe,
• Fatemeh Ghaderiardakani,
• Simona Fenizia,
• Aleš Svatoš,
• Georg Pohnert and
• Thomas Wichard

Beilstein J. Org. Chem. 2021, 17, 1313–1322, doi:10.3762/bjoc.17.91

• fixative and stain. All the experiments with glass slides were performed in biological triplicates. Genome analysis To identify the putative biosynthetic gene cluster (ect gene cluster) in U. mutabilis [34], the algal genome was searched for the gene ectoine hydroxylase (ectD) and also for a specialised

Lectins of Mycobacterium tuberculosis – rarely studied proteins

• Katharina Kolbe,
• Sri Kumar Veleti,
• Norbert Reiling and
• Thisbe K. Lindhorst

Beilstein J. Org. Chem. 2019, 15, 1–15, doi:10.3762/bjoc.15.1

• species [12]. The presence of mycobacterial lectins was further supported by Abhinav et al. using in silico genome analysis. A bioinformatics homology-based search of lectin-encoding gene regions in 30 fully or partially sequenced mycobacterial genomes identified 94 potential glycan-binding proteins. The

• ]. Interestingly, anti-HBHA antibodies have been detected in the sera of TB patients [82]. Thus, a humoral immune response to HBHA might also be connected to a reduced dissemination of Mtb from human lungs. Apart from the potential lectins predicted by in silico genome analysis, a C-type lectin-like carbohydrate

• . Interestingly, one of the T4P-associated genes is Rv3659, previously identified by in silico genome analysis as coding for a potential mycobacterial lectin (see above) [78]. Although the related protein is most likely involved in pili assembly, it is not inconceivable that T4P have carbohydrate-binding

Natural products from microbes associated with insects

• Christine Beemelmanns,
• Huijuan Guo,
• Maja Rischer and
• Michael Poulsen

Beilstein J. Org. Chem. 2016, 12, 314–327, doi:10.3762/bjoc.12.34

• citrus psyllid and the β-proteobacterium ”Candidatus Profftella armatura” [54][55]. A genome analysis of Profftella, which resides in a symbiotic organ called the bacteriome, revealed that 15% of the drastically reduced genome encoded horizontally acquired genes for the biosynthesis of the polyketide