Methyltransferases from RiPP pathways: shaping the landscape of natural product chemistry

• Maria-Paula Schröder,
• Isabel P.-M. Pfeiffer and
• Silja Mordhorst

Beilstein J. Org. Chem. 2024, 20, 1652–1670, doi:10.3762/bjoc.20.147

• process of biosynthesis, the quinaldic moiety is eventually integrated into the precursor peptide TsrA. Besides its unique methylation reaction, TsrM has a high turnover rate and shows a great substrate ambiguity [128]. TsrM was crystallised, revealing its structure, and enabling the localisation of bound

An improved synthesis of a fluorophosphonate–polyethylene glycol–biotin probe and its use against competitive substrates

• Hao Xu,
• Hairat Sabit,
• Gordon L. Amidon and
• H. D. Hollis Showalter

Beilstein J. Org. Chem. 2013, 9, 89–96, doi:10.3762/bjoc.9.12

• these competition studies demonstrate a new application of FP-based probes seldom explored before. Keywords: biotin; fluorophosphonate; high turnover rate; reversible substrate; Introduction One of the goals of chemical biology is to develop small–molecule- and biomolecule-based probes to interrogate

• by the Cravatt group [21]. Kinetic study of FP probe labelling activity An incubation-time control study is the key toward successfully monitoring the competition between FP probe 1 and a reversible substrate with a high turnover rate. Hence, over a long incubation time, the substrate will be

• human tissues, is shown to be active in the hydrolysis of both enalapril and oseltamivir [23][24]. Each is hydrolyzed at a high turnover rate. In a control run, pCES (± preheated) was treated with 4 μM FP–PEG–biotin 1 (Figure 4A, lanes 1 and 2). Then in subsequent runs, non-preheated samples of enzyme