Shape, membrane morphology, and morphodynamic response of metabolically active human mitochondria revealed by scanning ion conductance microscopy

Eric Lieberwirth, Anja Schaeper, Regina Lange, Ingo Barke, Simone Baltrusch and Sylvia Speller
Beilstein J. Nanotechnol. 2025, 16, 951–967. https://doi.org/10.3762/bjnano.16.73

Supporting Information

Further information to mitochondrial morphodynamics and the measurement setup; Supporting information provides further mitochondrial topographies and difference images, and detailed information about the measurement setup and microspheres. The following sections are included: S1: SICM measurements of metabolically active and fixed isolated mitochondria; S2: Bimodal distribution of the diameter of metabolically active mitochondria; S3: Calculated difference images of a MG-63 osteoblast, metabolically active, isolated mitochondria, and a 3 μm microsphere, as well as further calculations to visualise the edge memory effect; S4: Additional observations at metabolically active mitochondria; S5: Connection of ζ-potential and membrane fluctuations; S6: SEM measurements of the polystyrene microspheres; S7: Details of the imaging dishes and SICM setup; S8: SEM measurements and other details of the nanopipettes; S9: Further information about the hopping mode at our SICM setup.

Supporting Information File 1: Additional experimental data.
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Cite the Following Article

Shape, membrane morphology, and morphodynamic response of metabolically active human mitochondria revealed by scanning ion conductance microscopy
Eric Lieberwirth, Anja Schaeper, Regina Lange, Ingo Barke, Simone Baltrusch and Sylvia Speller
Beilstein J. Nanotechnol. 2025, 16, 951–967. https://doi.org/10.3762/bjnano.16.73

How to Cite

Lieberwirth, E.; Schaeper, A.; Lange, R.; Barke, I.; Baltrusch, S.; Speller, S. Beilstein J. Nanotechnol. 2025, 16, 951–967. doi:10.3762/bjnano.16.73

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