Mechanical properties of MDCK II cells exposed to gold nanorods

• Anna Pietuch,
• Bastian Rouven Brückner,
• David Schneider,
• Marco Tarantola,
• Christina Rosman,
• Carsten Sönnichsen and
• Andreas Janshoff

Beilstein J. Nanotechnol. 2015, 6, 223–231, doi:10.3762/bjnano.6.21

• ), respectively. The following day, excess PEG in solution was removed by centrifugation of the suspension and PEGylation was confirmed by gel electrophoresis [10][13][25]. Concentration of particles is given as concentration of gold in μg/mL or particle number per mL. Fluorescence and dark field imaging

The fate of a designed protein corona on nanoparticles in vitro and in vivo

• Denise Bargheer,
• Julius Nielsen,
• Gabriella Gébel,
• Markus Heine,
• Sunhild C. Salmen,
• Roland Stauber,
• Horst Weller,
• Joerg Heeren and
• Peter Nielsen

Beilstein J. Nanotechnol. 2015, 6, 36–46, doi:10.3762/bjnano.6.5

• surface was modified by PEGylation with mono- or bifunctional poly(ethylene oxide)amines (PEG). Using 125I-labeled test proteins (transferrin, albumin), the binding and exchange of corona proteins was studied first in vitro. Incubation with 125I-transferrin showed that with increasing grade of PEGylation

• reported to play significant roles [1][2][3][4][5][6][7]. A small size, neutral or negative zeta potential, and extended PEGylation of the surface material are correlated with increased circulation time in blood after intravenous (i.v.) injection [4][6]. One important implication is that NP upon contact

• proteins was diminished with the grade of PEGylation with no observable differences between adsorbed and covalently bound transferrin. After addition of a 3 fold excess of unlabeled bovine albumin, the same procedure of ultrafiltration and SEC-FPLC was performed (Figure 2). Again, a leakage of the adsorbed

Mammalian cell growth on gold nanoparticle-decorated substrates is influenced by the nanoparticle coating

• Christina Rosman,
• Sebastien Pierrat,
• Marco Tarantola,
• David Schneider,
• Eva Sunnick,
• Andreas Janshoff and
• Carsten Sönnichsen

Beilstein J. Nanotechnol. 2014, 5, 2479–2488, doi:10.3762/bjnano.5.257

• –PEG particles) or 75% COOH–PEG–SH and 25% CH3O–PEG–SH (COOH–PEG particles), respectively. The next day, excess PEG was removed by centrifugation. The success of the PEGylation was tested by gel electrophoresis, which also reveals the surface charge of the particles (Supporting Information of [20

The surface properties of nanoparticles determine the agglomeration state and the size of the particles under physiological conditions

• Christoph Bantz,
• Olga Koshkina,
• Thomas Lang,
• Hans-Joachim Galla,
• C. James Kirkpatrick,
• Roland H. Stauber and
• Michael Maskos

Beilstein J. Nanotechnol. 2014, 5, 1774–1786, doi:10.3762/bjnano.5.188

• verified under physiological conditions for the PEGylated samples, as described below. After PEGylation, the zeta potential drops from +30 mV to ca. +15 mV. The explanation for this observation is that the additional PEG layer leads to a shielding of the underlying positive charges. Characterization under

PEGylated versus non-PEGylated magnetic nanoparticles as camptothecin delivery system

• Paula M. Castillo,
• Mario de la Mata,
• Maria F. Casula,
• José A. Sánchez-Alcázar and
• Ana P. Zaderenko

Beilstein J. Nanotechnol. 2014, 5, 1312–1319, doi:10.3762/bjnano.5.144

• strategy depicted in Scheme 1. In a typical PEGylation procedure the surface of USM nanoparticles was first coated with succinic acid, in order to allow for the subsequent covalent linkage of bis(3-aminopropyl)-terminated poly(ethylene glycol) through carbodiimide chemistry. It is known that the use of

Sensing surface PEGylation with microcantilevers

• Natalija Backmann,
• Natascha Kappeler,
• Thomas Braun,
• François Huber,
• Hans-Peter Lang,
• Christoph Gerber and
• Roderick Y. H. Lim

Beilstein J. Nanotechnol. 2010, 1, 3–13, doi:10.3762/bjnano.1.2

• biotechnology on account of its good solubility in water and low toxicity. Polypeptide drugs and nanoparticles designed for drug delivery exhibit enhanced biocompatibility and proteolytic resistance (e.g., “stealth” particles) when modified with PEG (i.e., PEGylation) [1][2]. PEG-functionalized surfaces are

• conformation of a surface-grafted PEG layer, and how this might influence its interfacial properties may prove beneficial towards optimizing the PEGylation process. Although techniques such as X-ray photoelectron spectroscopy (XPS) [6][7] and ellipsometry [8] have been proven powerful in terms of studying

• surface PEGylation, and (2) conformational changes in the PEG layer over a timescale of tens of minutes in situ. Specifically, thiol-terminated PEG (mPEG–SH, 20 kDa) chains have been covalently tethered onto Au-coated microcantilever surfaces by the “grafting to” approach. When switching between good