Atomic force microscopy as analytical tool to study physico-mechanical properties of intestinal cells

• Christa Schimpel,
• Oliver Werzer,
• Eleonore Fröhlich,
• Gerd Leitinger,
• Markus Absenger-Novak,
• Birgit Teubl,
• Andreas Zimmer and
• Eva Roblegg

Beilstein J. Nanotechnol. 2015, 6, 1457–1466, doi:10.3762/bjnano.6.151

• , differing in lengths as well as diameters. This suggests that the absence of a well-developed brush border in M cells may facilitate the adherence of antigens on the cell surface and, as a consequence, cellular uptake processes [2]. By contrast, the large surface area of intestinal microvilli is more

• properties of cells can be directly linked to cell adhesion, adhesion to the smooth and more elastic surface of M cells is enhanced, thus, facilitating the adherence of antigens and, as a consequence, cellular uptake processes. Experimental Cell cultures Raji B cells were a kind gift from R. Fuchs (Medical

Characterization of nanostructured ZnO thin films deposited through vacuum evaporation

• Jose Alberto Alvarado,
• Arturo Maldonado,
• Héctor Juarez,
• Mauricio Pacio and
• Rene Perez

Beilstein J. Nanotechnol. 2015, 6, 971–975, doi:10.3762/bjnano.6.100

• 1000 °C for 1 h in air), in order to observe the evolution in the formation of these 2D nanostructures. Thin films were deposited using the vacuum metal evaporation system Jeol model JEE-420. The annealing process was done in a conventional oven to reorganize the crystallites and increase the adherence

• of particles that do not have an ordering, and thus the grain boundaries from each nanoparticle are not activated. Therefore, the adherence to the substrate is poor, which is also seen in the transmittance results. Varying the duration for which the films are annealed results in a perceptible

Hematopoietic and mesenchymal stem cells: polymeric nanoparticle uptake and lineage differentiation

• Ivonne Brüstle,
• Thomas Simmet,
• Gerd Ulrich Nienhaus,
• Katharina Landfester and
• Volker Mailänder

Beilstein J. Nanotechnol. 2015, 6, 383–395, doi:10.3762/bjnano.6.38

• (Fluka, Buchs, Switzerland) in a humidified incubator with 5% CO2 at 37 °C. For particle uptake and cell viability experiments, cells were seeded at a density of 10,000 cells/cm2 in 6-well plates (Greiner Bio, Frickenhausen, Germany). After adherence, the cells were incubated with 300 µg/mL nanoparticles

Increasing throughput of AFM-based single cell adhesion measurements through multisubstrate surfaces

• Miao Yu,
• Nico Strohmeyer,
• Jinghe Wang,
• Daniel J. Müller and
• Jonne Helenius

Beilstein J. Nanotechnol. 2015, 6, 157–166, doi:10.3762/bjnano.6.15

• adherence to various ECM proteins, necessitating the investigation of the adhesive properties of the cells. Atomic force microscopy (AFM)-based single-cell force spectroscopy (SCFS) provides a versatile tool to quantify the adhesion of single cells in near-physiological conditions [14][15][16]. In AFM-based

Mammalian cell growth on gold nanoparticle-decorated substrates is influenced by the nanoparticle coating

• Christina Rosman,
• Sebastien Pierrat,
• Marco Tarantola,
• David Schneider,
• Eva Sunnick,
• Andreas Janshoff and
• Carsten Sönnichsen

Beilstein J. Nanotechnol. 2014, 5, 2479–2488, doi:10.3762/bjnano.5.257

• spreading area at day two. This example is chosen as a representative of the average behavior in increase of the surface coverage with individual cells showing a smaller or even larger expansion compared to the presented cell. Adherence The adherence of cells was indicated by the amount of adherent cells

• reduction in adherence by 30% as compared to the untreated control results from the cell growth on the NH2–PEG nanorod-decorated substrate, whereas immobilization of COOH–PEG nanorods does not have an influence. Proliferation The increase in the spreading area of an adherent cell is interpreted as a sign of

• times) [30]. As only the CTAB nanorods display a significant impact on cell adherence and proliferation, we focus on these particles in the micromotion assay. In the assay, CTAB nanorods with mean particle densities from 65 to 2,000 particles/µm² were immobilized on the substrate and a baseline signal

Coating with luminal gut-constituents alters adherence of nanoparticles to intestinal epithelial cells

• Heike Sinnecker,
• Katrin Ramaker and
• Andreas Frey

Beilstein J. Nanotechnol. 2014, 5, 2308–2315, doi:10.3762/bjnano.5.239

• gut-constituents on the adherence of nanoparticles to intestinal epithelial cells. Carboxylated polystyrene particles 20, 100 and 200 nm in size represented our anthropogenic NPs, and differentiated Caco-2 cells served as model for mature enterocytes of the small intestine. Pretreatment with the

• proteins BSA and casein consistently reduced the adherence of all NPs to the cultured enterocytes, while incubation of NPs with meat extract had no obvious effect on particle adherence. In contrast, contact with intestinal fluid appeared to increase the particle-cell interaction of 20 and 100 nm NPs

• . Conclusion: Luminal gut-constituents may both attenuate and augment the adherence of NPs to cell surfaces. These effects appear to be dependent on the particle size as well as on the type of interacting protein. While some proteins will rather passivate particles towards cell attachment, possibly by

Antimicrobial nanospheres thin coatings prepared by advanced pulsed laser technique

• Alina Maria Holban,
• Valentina Grumezescu,
• Alexandru Mihai Grumezescu,
• Bogdan Ştefan Vasile,
• Roxana Truşcă,
• Rodica Cristescu,
• Gabriel Socol and
• Florin Iordache

Beilstein J. Nanotechnol. 2014, 5, 872–880, doi:10.3762/bjnano.5.99

• -CS-Fe3O4@EUG nanospheres diameter sizes range between 20 and 80 nm. These MAPLE-deposited coatings acted as bioactive nanosystems and exhibited a great antimicrobial effect by impairing the adherence and biofilm formation of Staphylococcus aureus (S. aureus) and Pseudomonas aeruginosa (P. aeruginosa

Hole-mask colloidal nanolithography combined with tilted-angle-rotation evaporation: A versatile method for fabrication of low-cost and large-area complex plasmonic nanostructures and metamaterials

• Jun Zhao,
• Bettina Frank,
• Frank Neubrech,
• Chunjie Zhang,
• Paul V. Braun and
• Harald Giessen

Beilstein J. Nanotechnol. 2014, 5, 577–586, doi:10.3762/bjnano.5.68

• hydrophobicity of the PMMA layer. Before drop coating of the polystyrene (PS) spheres, which have negative electric surface charges, we should bring at first a net positive charge to the polymer layer for a better adherence and arrangement. Here we use a poly(diallyldimethylammonium chloride) (PDDA) solution

Exploring the retention properties of CaF₂ nanoparticles as possible additives for dental care application with tapping-mode atomic force microscope in liquid

• Matthias Wasem,
• Joachim Köser,
• Sylvia Hess,
• Enrico Gnecco and
• Ernst Meyer

Beilstein J. Nanotechnol. 2014, 5, 36–43, doi:10.3762/bjnano.5.4

• nanoparticles are candidates for additives in dental care products as they could serve as fluorine-releasing containers preventing caries during a cariogenic acid attack on the teeth. We show that the adherence of the nanoparticles is increased on the enamel substrate compared to mica, independently of the

Forming nanoparticles of water-soluble ionic molecules and embedding them into polymer and glass substrates

• Stella Kiel,
• Olga Grinberg,
• Nina Perkas,
• Jerome Charmet,
• Herbert Kepner and
• Aharon Gedanken

Beilstein J. Nanotechnol. 2012, 3, 267–276, doi:10.3762/bjnano.3.30

• toward the substrate at such a high speed (>500 m/s) that it leads to the strong adherence of the NPs to the surface without the need for any binding agents [11][12]. In the present work we report for the first time on a novel general method for the preparation of water-soluble NPs of ionic salts. The