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Search for "biological fluids" in Full Text gives 45 result(s) in Beilstein Journal of Nanotechnology.
Dynamics of superparamagnetic nanoparticles in viscous liquids in rotating magnetic fields
Beilstein J. Nanotechnol. 2019, 10, 2294–2303, doi:10.3762/bjnano.10.221
- moments of the particle. However, if nanoparticles remain distributed in biological fluids (blood, serum), the intensity of AMF energy absorption is determined also by the rotation of the nanoparticles as a whole in a viscous liquid [25][26]. Various mathematical approaches are necessary for a theoretical
Microfluidics as tool to prepare size-tunable PLGA nanoparticles with high curcumin encapsulation for efficient mucus penetration
Beilstein J. Nanotechnol. 2019, 10, 2280–2293, doi:10.3762/bjnano.10.220
- our knowledge. Nanoparticle interaction with mucin The stability of NPs within biological fluids is an essential factor with respect to their potential biological effects [53]. This holds especially true for the interaction of the particles with mucus. To estimate this, a mucin solution was chosen as
Serum type and concentration both affect the protein-corona composition of PLGA nanoparticles
Beilstein J. Nanotechnol. 2019, 10, 1002–1015, doi:10.3762/bjnano.10.101
- few NPs have made it to clinical trials or market maturity [2][3]. One possible reason is the limited understanding of the interaction occurring at the interface between NPs and the physiological surrounding [3]. Once in contact with biological fluids, such as blood, proteins adsorb onto the surface
- incubation medium create a saturated NP surface for both biological fluids and a further increase in serum content does not lead to changes in the corona formation. Accordingly, this allowed us to reliably address the aforementioned research topic without considering the variabilities caused by phenomena
Polydopamine-coated Au nanorods for targeted fluorescent cell imaging and photothermal therapy
Beilstein J. Nanotechnol. 2019, 10, 794–803, doi:10.3762/bjnano.10.79
- designed parameters [3][4]. The AuNRs themselves can serve as contrast agents for two-photon [5][6], photoacoustic [7][8][9] and SERS [10][11] imaging, and for plasmonic photothermal therapy (PPT) [12][13]. However, the as-prepared AuNRs demonstrate high toxicity [14][15] and low stability in biological
- fluids because of a cetyltrimethylammonium bromide (CTAB) bilayer on the AuNR surface, which is a necessary agent in the synthesis method [16]. The coating of the nanoparticles with polymeric or inorganic shells and further functionalization with target molecules can help to overcome this drawback
Self-assembly of chiral fluorescent nanoparticles based on water-soluble L-tryptophan derivatives of p-tert-butylthiacalix[4]arene
Beilstein J. Nanotechnol. 2017, 8, 1825–1835, doi:10.3762/bjnano.8.184
- biologically important substrates and for analyzing biological fluids. Tryptophan has a maximum emission in water at 348 nm. The position of the maximum strongly depends on the polarity of the solvent [43]. The emission maximum of the compounds 8 and 9 in water was observed at 415 nm. It was shifted to the red
Recombinant DNA technology and click chemistry: a powerful combination for generating a hybrid elastin-like-statherin hydrogel to control calcium phosphate mineralization
Beilstein J. Nanotechnol. 2017, 8, 772–783, doi:10.3762/bjnano.8.80
- main advantage of recombinant DNA technology is the ability to control the distribution of lysines and the SNA15 bioactive domain along the recombinamer chain. Elastin-like recombinamer hydrogel formation Click reactions are a class of reaction that can often also be performed in biological fluids
A novel electrochemical nanobiosensor for the ultrasensitive and specific detection of femtomolar-level gastric cancer biomarker miRNA-106a
Beilstein J. Nanotechnol. 2016, 7, 2023–2036, doi:10.3762/bjnano.7.193
- ® Premix Ex Taq™ II (TAKARA BIO INC.) according to the protocol of the manufacturer. The obtained Ct values were considered for plotting the standard curve. Spiked sample analysis In order to study the nanobiosensor performance in biological fluids, the serum sample was used instead of hybridization buffer
Surface coating affects behavior of metallic nanoparticles in a biological environment
Beilstein J. Nanotechnol. 2016, 7, 246–262, doi:10.3762/bjnano.7.23
- biological media. The combination of negative charge and high adsorption strength of coating agents proved to be important for achieving good stability of metallic NPs in electrolyte-rich fluids. Most importantly, the presence of proteins provided colloidal stabilization to metallic NPs in biological fluids
- regardless of their chemical composition, surface structure and surface charge. In addition, an assessment of AgNP and SPION behavior in real biological fluids, rat whole blood (WhBl) and blood plasma (BlPl), revealed that the composition of a biological medium is crucial for the colloidal stability and type
- of metallic NP transformation. Our results highlight the importance of physicochemical characterization and stability evaluation of metallic NPs in a variety of biological systems including as many NP properties as possible. Keywords: biological fluids; colloidal stability; maghemite; nanoparticles
Au nanoparticle-based sensor for apomorphine detection in plasma
Beilstein J. Nanotechnol. 2015, 6, 2224–2232, doi:10.3762/bjnano.6.228
- toward small species, such as drug molecules, with respect to a more bulky chemical species, for example, proteins in biological fluids. It is noteworthy that the above-discussed features of the artificially roughened surface in Figure 1A are homogeneous over areas of the order of 5 × 103 nm2, as shown
- interacting with the gold surface. The rapid acquisition time, together with a relatively low laser power, represent promising conditions for a rapid method for the measurement of the concentration of drugs in biological fluids. A spot size of about 1 µm ensures the collection of an average SERS signal from
- transfer to APO detection in biological fluids. Experiments performed on unfiltered blood plasma with different APO concentrations proved the applicability of the proposed method to APO detection for samples of clinical origin. (A, B) SEM micrographs of the surface morphology of a gold substrate deposited
Protein corona – from molecular adsorption to physiological complexity
Beilstein J. Nanotechnol. 2015, 6, 857–873, doi:10.3762/bjnano.6.88
- was suggested that charge distributions on the protein surface, rather than the overall molecular charge, govern the Coulomb interactions between NP and protein [9]. This intriguing idea seems indeed very relevant, considering that the Debye length at the typical ionic strengths of biological fluids
Biological responses to nanoscale particles
Beilstein J. Nanotechnol. 2015, 6, 380–382, doi:10.3762/bjnano.6.37
- conjugates of biomolecules, magnetism, radioactivity, Janus particles and core–shell particles were combined. In particular, the use of fluorescently labeled particles has become one of the preferred tools to track nanoparticles inside cells and tissue. When nanoparticles are exposed to biological fluids
Functionalized polystyrene nanoparticles as a platform for studying bio–nano interactions
Beilstein J. Nanotechnol. 2014, 5, 2403–2412, doi:10.3762/bjnano.5.250
- ]. Polystyrene is biocompatible and is not expected to adversely affect interactions of nanoparticles with biological systems. Specifically surface-modified polystyrene nanoparticles are homogeneous, exhibit a low polydispersity index, and form stable colloids in biological fluids [34]. We have used polystyrene
Effects of surface functionalization on the adsorption of human serum albumin onto nanoparticles – a fluorescence correlation spectroscopy study
Beilstein J. Nanotechnol. 2014, 5, 2036–2047, doi:10.3762/bjnano.5.212
- attention. Considering the wide variety of existing NPs and the complexity of biological fluids, substantial variations in their mutual interactions can be expected. Knowledge of these interactions is, however, indispensible for safe applications of NPs in the field of nanomedicine, for example, as highly
Influence of surface-modified maghemite nanoparticles on in vitro survival of human stem cells
Beilstein J. Nanotechnol. 2014, 5, 1732–1737, doi:10.3762/bjnano.5.183
- stealth particles with reduced opsonization in biological fluids. These properties can be exploited in magnetic resonance imaging and tracking of iron oxide-labeled cells, for the magnetic separation of cells, nucleic acids and proteins and in medicine for treatments by using targeted drug delivery
In vitro and in vivo interactions of selected nanoparticles with rodent serum proteins and their consequences in biokinetics
Beilstein J. Nanotechnol. 2014, 5, 1699–1711, doi:10.3762/bjnano.5.180
- of the NP is determined by the types of proteins covering the NP. In other words, the physicochemical properties of the NP play an essential role during protein binding but the biokinetics is largely determined by the covering proteins and their dynamic exchange in various biological fluids. This
Current state of laser synthesis of metal and alloy nanoparticles as ligand-free reference materials for nano-toxicological assays
Beilstein J. Nanotechnol. 2014, 5, 1523–1541, doi:10.3762/bjnano.5.165
- controlled independently while their uniquely high purity is retained. Hence, this article will highlight totally surfactant-free size control strategies for laser-fabricated nanoparticles and will comment on the stability of these particles in biological fluids. Additionally, laser-based synthesis methods
- particle concentrations. An electrostatically-controlled approach for ligand-free size control of gold nanoparticle is the in situ addition of simple inorganic electrolytes like NaCl or sodium phosphate buffer. These additives are frequently found in most biological fluids and hence are not prone to
- salinities, however, are not realistic for toxicological assays and are solely required during synthesis. Hence, upon exposition to biological fluids like blood, significantly higher ionic strengths (≈200 mM) may be found. This means that upon addition of the nanoparticles to biological fluids, 1/κ is
The protein corona protects against size- and dose-dependent toxicity of amorphous silica nanoparticles
Beilstein J. Nanotechnol. 2014, 5, 1380–1392, doi:10.3762/bjnano.5.151
- major factor (co)determining their biological identity and hence, effects at the nano–bio interface [22]. In biological fluids, proteins bind to the surface of nanoparticles to form a biological coating around the nanoparticle, known as the protein corona. Over the time, this corona evolves and may
Mimicking exposures to acute and lifetime concentrations of inhaled silver nanoparticles by two different in vitro approaches
Beilstein J. Nanotechnol. 2014, 5, 1357–1370, doi:10.3762/bjnano.5.149
- into the environment (such as for instance air or water, experimental media or biological fluids) are subject to a number of processes (such as aggregation and oxidation with the formation of Ag+) that alter their physico-chemical characteristics. These possible processes influence the mode of
Characterization of protein adsorption onto FePt nanoparticles using dual-focus fluorescence correlation spectroscopy
Beilstein J. Nanotechnol. 2011, 2, 374–383, doi:10.3762/bjnano.2.43
- due to their large surface-to-volume ratio [3] and, therefore, NPs may also pose a biological hazard [4][5]. Upon incorporation into the body, NPs become exposed to biological fluids such as lung epithelial lining fluid or blood plasma, which contain a variety of dissolved molecules, especially
Magnetic nanoparticles for biomedical NMR-based diagnostics
Beilstein J. Nanotechnol. 2010, 1, 142–154, doi:10.3762/bjnano.1.17
- these advantages, the sample volume for DMR detection could be reduced by a factor of ~10 (to 1 µL) compared to the previous devices (~10 µL). The microfluidic networks in the DMR system facilitate the handling of biological fluids, the effective mixing of MNPs with small sample volumes, and the
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