Cardiomyocyte uptake mechanism of a hydroxyapatite nanoparticle mediated gene delivery system

• Hiroaki Komuro,
• Masahiro Yamazoe,
• Kosuke Nozaki,
• Akiko Nagai and
• Tetsuo Sasano

Beilstein J. Nanotechnol. 2020, 11, 1685–1692, doi:10.3762/bjnano.11.150

• regarding safety has attracted significant attention [4][5]. The calcium phosphate (CaP) co-precipitation method has been extensively used for gene delivery due to its excellent biocompatibility and simple preparation [6]. CaP is commonly considered as one of the most important inorganic materials for

• , this method may provide a solution to the current problem of using calcium phosphate. The cellular uptake performance is important for a successful vector-mediated gene transfection. In the cellular uptake process, the internalization pathway is an essential factor to prevent the fate of lysosomal

• the presence than in the absence of HAp (Figure 5). Extracellular calcium ions are proposed to act as a stimulant for triggering macropinocytosis in macrophages and neurons [25][36]. The extracellular calcium-sensing receptor (CaSR) is expressed in cardiomyocytes as well as in various other cells [37

PTCDA adsorption on CaF₂ thin films

• Philipp Rahe

Beilstein J. Nanotechnol. 2020, 11, 1615–1622, doi:10.3762/bjnano.11.144

• on CaF2(111) of nearly flat-lying PTCDA molecules with two oxygen atoms displaced towards calcium surface ions. This geometry is in agreement with the experimental observations. Keywords: calcium difluoride; decoupling; insulating thin film; 3,4,9,10-perylene tetracarboxylic dianhydride (PTCDA

• flat-lying geometry is found from geometry-optimisation calculations of a single PTCDA molecule on a CaF2 slab using DFT, whereby an interaction between two carbonyl oxygen atoms and two surface calcium ions leads to a slight deformation of the PTCDA molecule. Methods Sample preparation and STM

• that two of the four carbonyl oxygen atoms are displaced out of the PTCDA plane towards a surface calcium ion. An attraction between the molecular oxygen and surface calcium atoms is suggested from an oxygen–calcium distance of about 2.8 Å. Note that this distance is larger than 2.4 Å recently found

Protruding hydrogen atoms as markers for the molecular orientation of a metallocene

• Linda Laflör,
• Michael Reichling and
• Philipp Rahe

Beilstein J. Nanotechnol. 2020, 11, 1432–1438, doi:10.3762/bjnano.11.127

• of the ferrocene moiety, herein on CaF2(111) surfaces, by using the protruding hydrogen atoms as markers. Keywords: calcium fluoride (CaF2); ferrocene; functionalised tips; high-resolution imaging; non-contact atomic force microscopy; Introduction It is still a challenge to determine the precise

Transient coating of γ-Fe₂O₃ nanoparticles with glutamate for its delivery to and removal from brain nerve terminals

• Konstantin Paliienko,
• Artem Pastukhov,
• Michal Babič,
• Daniel Horák,
• Olga Vasylchenko and
• Tatiana Borisova

Beilstein J. Nanotechnol. 2020, 11, 1381–1393, doi:10.3762/bjnano.11.122

• [39]. The neuronal release of glutamate modulates the blood–brain barrier function, through activation of N-methyl-ᴅ-aspartate (NMDA) receptors [40]. Glutamate increased intracellular calcium levels in endothelial cells and levels of nitrogen oxide (NO) around microvessels. These results can be

• considered in support of a mechanism of glutamate-induced activation of NMDA receptors in endothelial cells, which leads to calcium signaling and downstream NO production to promote blood–brain barrier permeability [38]. Thus, it may be expected that glutamate-conjugated γ-Fe2O3 nanoparticles can more easily

Applications of superparamagnetic iron oxide nanoparticles in drug and therapeutic delivery, and biotechnological advancements

• Maria Suciu,
• Corina M. Ionescu,
• Alexandra Ciorita,
• Septimiu C. Tripon,
• Dragos Nica,
• Hani Al-Salami and
• Lucian Barbu-Tudoran

Beilstein J. Nanotechnol. 2020, 11, 1092–1109, doi:10.3762/bjnano.11.94

Plant growth regulation by seed coating with films of alginate and auxin-intercalated layered double hydroxides

• Vander A. de Castro,
• Valber G. O. Duarte,
• Danúbia A. C. Nobre,
• Geraldo H. Silva,
• Vera R. L. Constantino,
• Frederico G. Pinto,
• Willian R. Macedo and
• Jairo Tronto

Beilstein J. Nanotechnol. 2020, 11, 1082–1091, doi:10.3762/bjnano.11.93

• All reagents used in this work were of analytical purity: Zn(NO3)2·6H2O (96.0%) and Al(NO3)3·9H2O (98.5%) were provided by Dinâmica; 1-naphthaleneacetic acid (C12H10O2, 97.0%), acetonitrile (C2H3N, 99.8%), sodium alginate (NaC6H7O6, >99.0%) and calcium nitrate (Ca(NO3)2, (99.0%) were supplied by Vetec

A 3D-polyphenylalanine network inside porous alumina: Synthesis and characterization of an inorganic–organic composite membrane

• Jonathan Stott and
• Jörg J. Schneider

Beilstein J. Nanotechnol. 2020, 11, 938–951, doi:10.3762/bjnano.11.78

• over calcium hydride and sodium/benzophenone, respectively. For recrystallization n-pentane was dried by distillation over sodium/potassium eutectic alloy. SEM measurements were performed with an XL30 FEG (Philips) system. Contact angles and NIR-spectroscopic analysis were performed with DSA30 (Krüss

Three-dimensional solvation structure of ethanol on carbonate minerals

• Hagen Söngen,
• Ygor Morais Jaques,
• Peter Spijker,
• Christoph Marutschke,
• Stefanie Klassen,
• Ilka Hermes,
• Ralf Bechstein,
• Lidija Zivanovic,
• John Tracey,
• Adam S. Foster and
• Angelika Kühnle

Beilstein J. Nanotechnol. 2020, 11, 891–898, doi:10.3762/bjnano.11.74

• bind towards calcite with their hydroxy group placed in between a surface calcium ion and a surface carbonate: The oxygen of the hydroxy group binds towards a calcium ion, while the hydrogen of the hydroxy group binds towards a carbonate group, which is similar to the bonding configuration of an

• between a calcium and a carbonate ion. This is in accordance with the previous MD simulations [14][15][16][17], which have shown that ethanol arranges in a ordered first layer above calcite, where one ethanol molecule binds towards one CaCO3 unit. The oxygen is closer to the cation (Ca/Mg) and the

• ), hydroxy–oxygen (red) and hydroxy–hydrogen (blue) at the (a) calcite–ethanol and (c) magnesite–ethanol interface. The graphs were created by drawing a semi-transparent dot for each atom at each time step of the simulation. The surfaces are represented by the carbonate groups (grey) and calcium (yellow) and

Multilayer capsules made of weak polyelectrolytes: a review on the preparation, functionalization and applications in drug delivery

• Varsha Sharma and
• Anandhakumar Sundaramurthy

Beilstein J. Nanotechnol. 2020, 11, 508–532, doi:10.3762/bjnano.11.41

• size and dissolve under mild conditions at pH < 3 or by using ethylenediaminetetraacetic acid (EDTA) without affecting the PE multilayer shell. They do not form any complexes with PEs and provided intact hollow capsules after core dissolution [30]. Cores such as calcium, cadmium and manganese carbonate

• capsules from high osmotic pressure and for encapsulation of macromolecules [32]. Calcium carbonate (CaCO3) cores have been widely used with many combinations of weak PEs to fabricate hollow capsules as in case of PAH/PMA capsules, Figure 2e [29]. More recently, hybrid CaCO3 templates built in with other

Facile biogenic fabrication of hydroxyapatite nanorods using cuttlefish bone and their bactericidal and biocompatibility study

• Satheeshkumar Balu,
• Manisha Vidyavathy Sundaradoss,
• Swetha Andra and
• Jaison Jeevanandam

Beilstein J. Nanotechnol. 2020, 11, 285–295, doi:10.3762/bjnano.11.21

• Department of Chemical Engineering, Curtin University, Miri, Sarawak 98009, Malaysia 10.3762/bjnano.11.21 Abstract Cuttlefish bones are an inexpensive source of calcium carbonate, which are produced in large amounts by the marine food industry, leading to environmental contamination and waste. The

• nontoxicity, worldwide availability and low production cost of cuttlefish bone products makes them an excellent calcium carbonate precursor for the fabrication of hydroxyapatite. In the present study, a novel oil-bath-mediated precipitation method was introduced for the synthesis of hydroxyapatite (Hap

• ; cuttlefish bone; hard tissue treatment; hydroxyapatite; nanorods; Introduction Generally, the hard tissue of humans and animals, such as bone and teeth, are composed of natural hydroxyapatite (Hap), which is a bioactive ceramic material with high calcium phosphate concentration whereby the material can

Rational design of block copolymer self-assemblies in photodynamic therapy

• Maxime Demazeau,
• Laure Gibot,
• Anne-Françoise Mingotaud,
• Patricia Vicendo,
• Clément Roux and
• Barbara Lonetti

Beilstein J. Nanotechnol. 2020, 11, 180–212, doi:10.3762/bjnano.11.15

Advanced hybrid nanomaterials

• Andreas Taubert,
• Fabrice Leroux,
• Pierre Rabu and
• Verónica de Zea Bermudez

Beilstein J. Nanotechnol. 2019, 10, 2563–2567, doi:10.3762/bjnano.10.247

• nanostructuration and related issues. Nanofillers Nanocomposites remain a vast playground for research into new hybrid systems. A common approach for polymer fillers presented in this thematic issue is their use as organo-modified layered double hydroxides (LDHs) or new layered calcium phenylphosphonates, as well

• hydrophobicity and lipophilicity. Exfoliated nanosheets of layered calcium phenylphosphonate assisted by solvent were used in “Layered calcium phenylphosphonate: a hybrid material for a new generation of nanofillers” to promote the mechanical properties and improve the barrier effect for applications such as

Bombesin receptor-targeted liposomes for enhanced delivery to lung cancer cells

• Mohammad J. Akbar,
• Pâmela C. Lukasewicz Ferreira,
• Melania Giorgetti,
• Leanne Stokes and
• Christopher J. Morris

Beilstein J. Nanotechnol. 2019, 10, 2553–2562, doi:10.3762/bjnano.10.246

• , Tyr4-Bn. A dose-dependent increase in intracellular calcium release was observed for NCI-H345 but not the GRPR deficient SCLC line, NCI-H82 (Figure 1a,b), as previously reported [17][18]. The physiological role of GRPR includes the stimulation of a mitogenic response after receptor internalisation [19

• ). Sources of other exceptional items are mentioned in the text. Calcium release assay NCI-H345 and NCI-H82 cells were loaded with 2 µM Fura-2AM (Thermo Fisher, UK) in Hank’s Balanced Salt Solution (HBSS) loading buffer containing sulfinpyrazone at 37 °C for 40–60 minutes. Following loading, the buffer was

Synthesis of highly active ETS-10-based titanosilicate for heterogeneously catalyzed transesterification of triglycerides

• Muhammad A. Zaheer,
• David Poppitz,
• Khavar Feyzullayeva,
• Marianne Wenzel,
• Jörg Matysik,
• Radomir Ljupkovic,
• Aleksandra Zarubica,
• Alexander A. Karavaev,
• Andreas Pöppl,
• Roger Gläser and
• Muslim Dvoyashkin

Beilstein J. Nanotechnol. 2019, 10, 2039–2061, doi:10.3762/bjnano.10.200

• removal [13]. Amongst the prospective solid catalysts designed for transesterification reactions, such as calcium [14] and other metal oxides [15], metal–organic frameworks (MOFs) [10], silica-supported catalysts [16], biochar [17] and other biomass-derived catalysts [18], zeolites and molecular sieves

• outperform them in terms of stability and ability for regeneration. Experimental Materials and chemicals used in the preparation of CaO- and ETS-10-based catalysts Calcium oxide of technical grade (Centrochem Co., Serbia) was used as a precursor in the preparation of CaO-based catalysts. For the preparation

Long-term entrapment and temperature-controlled-release of SF₆ gas in metal–organic frameworks (MOFs)

• Hana Bunzen,
• Andreas Kalytta-Mewes,
• Leo van Wüllen and
• Dirk Volkmer

Beilstein J. Nanotechnol. 2019, 10, 1851–1859, doi:10.3762/bjnano.10.180

• to obtain kinetic parameters during a mass loss [30][31]. Until now it has been mainly used to study organic polymers (e.g., poly(ethylene) and poly(styrene)) and simple inorganic compounds (e.g., calcium carbonate and calcium oxalate) [31]. Here we used the method to estimate the activation energy

Tailoring the stability/aggregation of one-dimensional TiO₂(B)/titanate nanowires using surfactants

• Atiđa Selmani,
• Johannes Lützenkirchen,
• Kristina Kučanda,
• Dario Dabić,
• Engelbert Redel,
• Ida Delač Marion,
• Damir Kralj,
• Darija Domazet Jurašin and
• Maja Dutour Sikirić

Beilstein J. Nanotechnol. 2019, 10, 1024–1037, doi:10.3762/bjnano.10.103

• different solution and interfacial behavior compared to corresponding conventional monomeric surfactants [32][33][34]. The interaction of gemini surfactants with solid (nano)surfaces such as clay [35], calcium phosphate [36], silica [37][38][39][40], TiO2 [41], ZnO [42] and carbon NTs [43] have been

Ultrathin hydrophobic films based on the metal organic framework UiO-66-COOH(Zr)

• Miguel A. Andrés,
• Clemence Sicard,
• Christian Serre,
• Olivier Roubeau and
• Ignacio Gascón

Beilstein J. Nanotechnol. 2019, 10, 654–665, doi:10.3762/bjnano.10.65

• OPD/MOF ultrathin films have been fabricated onto glass, calcium fluoride, quartz crystal microbalance (QCM), Si(100) substrates and mica and characterized using scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), atomic force microscopy (AFM

• inspection. IR spectra were obtained using a Perking Elmer Spectrum 100 spectrometer operated in transmission mode for films and in attenuated total reflectance (ATR) mode for powder samples. The films were deposited onto calcium fluoride windows. Water contact angle measurements were performed on different

Targeting strategies for improving the efficacy of nanomedicine in oncology

• Gonzalo Villaverde and
• Alejandro Baeza

Beilstein J. Nanotechnol. 2019, 10, 168–181, doi:10.3762/bjnano.10.16

• the control of the intracellular calcium concentration or the removing of the oxidative species, which could damage the cell. Therefore, the specific delivery of toxic species to these key organelles compromises the function of the entire cell causing its destruction. Yoong et al. have decorated the

Characterization and influence of hydroxyapatite nanopowders on living cells

• Przemyslaw Oberbek,
• Tomasz Bolek,
• Adrian Chlanda,
• Seishiro Hirano,
• Sylwia Kusnieruk,
• Julia Rogowska-Tylman,
• Ganna Nechyporenko,
• Viktor Zinchenko,
• Wojciech Swieszkowski and
• Tomasz Puzyn

Beilstein J. Nanotechnol. 2018, 9, 3079–3094, doi:10.3762/bjnano.9.286

• (OH)2) is a calcium phosphate, structurally and chemically similar to the mineral phase of human bone and teeth. Due to its high biocompatibility and bioactivity, it has been successfully applied in the manufacturing of cosmetics and hygiene products, as well as in bone-tissue engineering and

• regenerative medicine. The use of nanosized hydroxyapatites in biomedical applications is constantly growing due to their good mechanical properties and enhanced efficiency of gene transfection in drug delivery. Calcium phosphates are sensitive to the preparation conditions [11][12][13][14][15]. They can be

• ], with and without the admixture of fluorine (CaHAP300 and CaHFAP300, respectively) and with subsequent filter drying. Hydroxyapatites from the Laboratory of Nanostructures and from the Bogatsky Institute were synthesized with a calcium deficiency. The Ca/P atomic ratio of the obtained HAp samples is

Co-intercalated layered double hydroxides as thermal and photo-oxidation stabilizers for polypropylene

• Qian Zhang,
• Qiyu Gu,
• Fabrice Leroux,
• Pinggui Tang,
• Dianqing Li and
• Yongjun Feng

Beilstein J. Nanotechnol. 2018, 9, 2980–2988, doi:10.3762/bjnano.9.277

• Auvergne, Institut de Chimie de Clermont-Ferrand ICCF, UMR-CNRS 6296, F 63171 Aubière, France 10.3762/bjnano.9.277 Abstract An elegant and efficient approach consisting in the co-intercalation of stabilizing molecular anions is described here. The thermal stabilizer calcium diethyl bis[[[3,5-bis(1,1

Hybrid Au@alendronate nanoparticles as dual chemo-photothermal agent for combined cancer treatment

• Anouchka Plan Sangnier,
• Romain Aufaure,
• Laurence Motte,
• Claire Wilhelm,
• Erwann Guenin and
• Yoann Lalatonne

Beilstein J. Nanotechnol. 2018, 9, 2947–2952, doi:10.3762/bjnano.9.273

• phosphonate groups. The BP affinity for calcium is improved by adding a hydroxy (–OH) group, for instance in HMBP (hydroxylmethylene bisphosphonate), allowing for a tridentate coordination to Ca2+ ions (Supporting Information File 1, Figure S4) and leading to a high affinity to bone (hydroxyapatite (Ca10(PO4

Layered calcium phenylphosphonate: a hybrid material for a new generation of nanofillers

• Kateřina Kopecká,
• Ludvík Beneš,
• Klára Melánová,
• Vítězslav Zima,
• Petr Knotek and
• Kateřina Zetková

Beilstein J. Nanotechnol. 2018, 9, 2906–2915, doi:10.3762/bjnano.9.269

• /bjnano.9.269 Abstract The use of nanosheets of layered calcium phenylphosphonate as a filler in a polymeric matrix was investigated. Layered calcium phenylphosphonate (CaPhP), with chemical formula CaC6H5PO3∙2H2O, is a hybrid organic–inorganic material that exhibits a hydrophobic character due to the

• organophosphonates have been prepared. Well-known and well-studied are the organophosphonates of zirconium because of their good stability; however, it is also possible to prepare layered structures with divalent metals such as calcium, strontium or barium [6][7][8][9]. Although the properties of these compounds

• forces. The main advantage is that a ready-to-use dispersion of nanoplatelets is obtained, so the step of dispersing dry nanoparticles in a polymer matrix is avoided, which is usually challenging, and thus simplifies the preparation of the polymer composites. In this work, layered calcium

Enhanced antineoplastic/therapeutic efficacy using 5-fluorouracil-loaded calcium phosphate nanoparticles

• Shanid Mohiyuddin,
• Saba Naqvi and
• Gopinath Packirisamy

Beilstein J. Nanotechnol. 2018, 9, 2499–2515, doi:10.3762/bjnano.9.233

• , retention, and low toxicity, as well as surface engineering with targeting moieties, can be used as a tool in enhancing the therapeutic efficacy of current approaches. Inorganic calcium phosphate nanoparticles have the potential to increase the therapeutic potential of antiproliferative drugs due to their

• excellent loading efficiency, biodegradable nature and controlled-release behaviour. Herein, we report a novel system of 5-fluorouracil (5-FU)-loaded calcium phosphate nanoparticles (CaP@5-FU NPs) synthesized via a reverse micelle method. The formation of monodispersed, spherical, crystalline nanoparticles

• upon CaP@5-FU NP treatment. Likewise, the cell cycle analysis was performed to confirm the enhanced apoptotic induction. Our study concludes that the calcium phosphate nanocarriers system, i.e. CaP@5-FU NPs, has higher antineoplastic potential as compared to 5-FU alone and can be used as an improved

Nanocellulose: Recent advances and its prospects in environmental remediation

• Katrina Pui Yee Shak,
• Yean Ling Pang and
• Shee Keat Mah

Beilstein J. Nanotechnol. 2018, 9, 2479–2498, doi:10.3762/bjnano.9.232

• nanocellulose for example, is desired due to their good performance in the removal of organic pollutants. Hokkanen et al. [88] reported that calcium hydroxyapatite-microfibrillated cellulose adsorbent was effective for the removal of chromium (Cr(VI)) from aqueous solution. They found that calcium

The role of adatoms in chloride-activated colloidal silver nanoparticles for surface-enhanced Raman scattering enhancement

• Nicolae Leopold,
• Andrei Stefancu,
• Krisztian Herman,
• István Sz. Tódor,
• Stefania D. Iancu,
• Vlad Moisoiu and
• Loredana F. Leopold

Beilstein J. Nanotechnol. 2018, 9, 2236–2247, doi:10.3762/bjnano.9.208

• ), hydrogen peroxide (10% w/w solution, AppliChem), sodium citrate dihydrate (99%, Sigma-Aldrich), hydroxylamine hydrochloride (99%, Sigma-Aldrich), magnesium sulfate (99%, Sigma-Aldrich), calcium nitrate tetrahydrate (99%, Sigma-Aldrich), potassium iodide (99%, Sigma-Aldrich), crystal violet chloride (Reag