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Search for "cell adhesion" in Full Text gives 54 result(s) in Beilstein Journal of Nanotechnology.
Cytotoxicity of doxorubicin-conjugated poly[N-(2-hydroxypropyl)methacrylamide]-modified γ-Fe2O3 nanoparticles towards human tumor cells
Beilstein J. Nanotechnol. 2018, 9, 2533–2545, doi:10.3762/bjnano.9.236
- , however, still under study. It can be concluded that the newly designed γ-Fe2O3@P(HPMA-MMAA)-Dox nanoparticles are highly promising for the delivery of cancer medications into tumors, offering enhanced cell adhesion, increased apoptosis, minimal immunogenicity, lipid peroxidation, DNA damage, and reduced
High-throughput micro-nanostructuring by microdroplet inkjet printing
Beilstein J. Nanotechnol. 2018, 9, 2372–2380, doi:10.3762/bjnano.9.222
- BCML can be biofunctionalized such that they serve as biomimetic anchorage sites for cell adhesion molecules, whereby their spacing has been shown to be highly decisive for cell adhesion [14]. Still, the anchorage site spacing required for cell adhesion depends on the chemistry of a particular adhesion
- ligand [15]. It has even been reported that cells can respond to differences in ligand spacing as small as 1 nm across the cell diameter [16]. Therefore, the fabrication of complex patterns of gold nanoparticles is highly relevant in the context of the biomimicry of cell adhesion environments. Whereas
Biomimetic and biodegradable cellulose acetate scaffolds loaded with dexamethasone for bone implants
Beilstein J. Nanotechnol. 2018, 9, 1986–1994, doi:10.3762/bjnano.9.189
- . Cytotoxicity studies were performed by using MTT assay, methylene-blue staining and SEM fixation and showed very good cell adhesion and proliferation, indicating the cytocompatibility of these fibrous scaffolds. Drug-release kinetics was measured for the evaluation of a controllable and sustained release of
Preparation of micro/nanopatterned gelatins crosslinked with genipin for biocompatible dental implants
Beilstein J. Nanotechnol. 2018, 9, 1735–1754, doi:10.3762/bjnano.9.165
- of the surface of biomaterials. Surface topographical patterns significantly affect cell adhesion, spreading, morphology, proliferation, and differentiation [1][2][3][4][5]. Surfaces with specific micro/nanopatterns have been developed in order to reduce platelet response [6], to regulate stem cell
- shapes, using genipin crosslinking, can easily fabricate “sharp” patterns that can control the morphology and vinculin expression of cells grown on these surfaces. This control of cell shape and vinculin expression by patterning is known to be able to control cell adhesion, function, and differentiation
Nanoparticle delivery to metastatic breast cancer cells by nanoengineered mesenchymal stem cells
Beilstein J. Nanotechnol. 2018, 9, 321–332, doi:10.3762/bjnano.9.32
- -negative/QD-positive cells represented cancer cells that have taken up the QDs released from MSCs during 3D co-culture (Figure 7). The proof of principle was additionally confirmed using cancer-cell-associated marker epithelial cell adhesion molecule (EpCAM) in nanoengineered MSC and MCF7 co-culture
Uptake and intracellular accumulation of diamond nanoparticles – a metabolic and cytotoxic study
Beilstein J. Nanotechnol. 2017, 8, 1649–1657, doi:10.3762/bjnano.8.165
- viability and the cell number to decrease by 80–85%. The oxidation of the NDs hindered the decrease, but on day 7, a further decrease was observed. While the O-terminated NDs showed mechanical obstruction of cells by agglomerates preventing cell adhesion, migration and division, the H-terminated detonation
- reactive oxygen species. Alternatively, it could have been caused just by mechanical obstruction of the cell adhesion and division by ND agglomerates, as confirmed by live-cell imaging. A similar effect was also observed in human osteoblast-like MG 63 cells cultured in a medium with multiwalled carbon
- loose serum protein/ND aggregates or in cell debris. The well-spread osteoblasts are homogeneously and confluently distributed. Good cell adhesion and division confirms the viability of the cells with photoluminescent NDs (AR-40). Conclusion A comparison of cell viability with various types of NDs (HPHT
Silicon microgrooves for contact guidance of human aortic endothelial cells
Beilstein J. Nanotechnol. 2017, 8, 675–681, doi:10.3762/bjnano.8.72
- cell adhesion, morphology and proliferation were assessed, by comparing them to flat silicon substrates, used as control condition. Using human aortic endothelial cells, microscopy images demonstrate that the cellular response is different depending on the silicon surface, when it comes to cell
- -shaped features that consist of repeated ridges and grooves pattern. Surfaces with these geometries have been used to demonstrate the influence on cell adhesion, alignment and organization [18][19][20][21]. Differences in cytoskeleton elongation have been demonstrated between cells elongated on these
- , macroporous and micropillars, to study the effect of topography on the behaviour of endothelial cells. Collagen was found to stimulate cell adhesion and promote an enhanced cell attachment [32][33]. Herein, to mimic the elongated endothelium in natural lineal vessels, human aortic endothelial cells (HAECs
Nano- and microstructured materials for in vitro studies of the physiology of vascular cells
Beilstein J. Nanotechnol. 2016, 7, 1620–1641, doi:10.3762/bjnano.7.155
- development of micro- and nanofabrication techniques has permitted the manufacturing of precise surface topographies of materials surfaces. Samples with specific surface features haven been widely used for in vitro cell biology studies either to manipulate cell adhesion and resulting cell responses or to give
- in its surface chemistry since biological cell adhesion via integrins or other adhesion molecules will generally not directly occur to inorganic or organic polymeric materials. Thus, further modification of the surface with adhesive molecules, for example with proteins from the extra cellular matrix
- following section, a brief summary of the most common methods for control of the surface biochemistry and of the mechanical properties of the substrate are given. 1.5 Surface (bio)functionalization The surface (bio)chemistry of a material may regulate cell adhesion, survival, proliferation and
Viability and proliferation of endothelial cells upon exposure to GaN nanoparticles
Beilstein J. Nanotechnol. 2016, 7, 1330–1337, doi:10.3762/bjnano.7.124
- lower cellular mobility. Nevertheless, cellular proliferation does not seem to be affected as cells continue to divide even when a relatively large number of nanoparticles are internalized. Fixing nanoparticles to nonadherent, biocompatible silicone, we showed an enhancement in cell adhesion on surfaces
Advanced atomic force microscopy techniques III
Beilstein J. Nanotechnol. 2016, 7, 1052–1054, doi:10.3762/bjnano.7.98
- -cell force spectroscopy is used by a biophysics group around Jonne Helenius to quantify the contribution of cell adhesion to specific substrates at both the cell and single molecule level [19]. Furthermore, physico-mechanical properties of intestinal cells were elucidated by force curve measurements by
Improved biocompatibility and efficient labeling of neural stem cells with poly(L-lysine)-coated maghemite nanoparticles
Beilstein J. Nanotechnol. 2016, 7, 926–936, doi:10.3762/bjnano.7.84
- electrostatic interaction between negatively charged ions of the cell membrane and the surface of the culture plate. Due to the presence of NH2 groups, which promote cell adhesion, PLL is as well used as a non-viral transfection agent for gene delivery and DNA complexation [20]. Our previous studies showed that
Electrochemical coating of dental implants with anodic porous titania for enhanced osteointegration
Beilstein J. Nanotechnol. 2015, 6, 2183–2192, doi:10.3762/bjnano.6.224
- same time, it has also been observed in in-vitro experiments that too small nanopores can even be detrimental to living cell adhesion, as they may give rise to a kind of hydrophobic and thus antiwetting behavior [27]. In Figure 3b, the EDS analysis confirms the presence of Ti oxide on the surface. In
Atomic force microscopy as analytical tool to study physico-mechanical properties of intestinal cells
Beilstein J. Nanotechnol. 2015, 6, 1457–1466, doi:10.3762/bjnano.6.151
- available at the cell edge. The elasticity of M cells was 1.7-fold higher compared to Caco-2 cells and increased significantly from the cell periphery to the nuclear region. Since elasticity can be directly linked to cell adhesion, M cells showed higher adhesion forces than Caco-2 cells. The combination of
- can be directly linked to cell adhesion, since indentation also determines the number of adhesive bonds which are formed between cells and a surface. Hence, a smaller indentation and a consequent reduced contact area leads to a decrease in cellular adhesion [54]. Thus, we zoomed into the region, where
- interactions) [56][57][58]. However, the cell adhesion molecule α5β1 integrin exhibits a different distribution pattern in M cells compared to enterocytes. Enterocytes display integrin only on basolateral and lateral surfaces, whereas M cells express α5β1 integrin on the apical membrane [41]. It is known that
Polymer blend lithography for metal films: large-area patterning with over 1 billion holes/inch2
Beilstein J. Nanotechnol. 2015, 6, 1205–1211, doi:10.3762/bjnano.6.123
- hierarchical micro–nano structures for applications such as cell-adhesion studies [36][37]. Metals like Cr, Au or Cu are good etching resists. Therefore the metal masks could be used to amplify the topographic contrast by anisotropic etching into the substrate with techniques such as reactive ion etching [38
- be used for various applications, e.g., in cell adhesion studies, for the immobilization of biomaterials, for plasmonics such as optical filters or as resist layers for anisotropic reactive ion etching. The wavelength-selective optical transmission of our perforated films due to the localized surface
Self-assembled anchor layers/polysaccharide coatings on titanium surfaces: a study of functionalization and stability
Beilstein J. Nanotechnol. 2015, 6, 617–631, doi:10.3762/bjnano.6.63
- titanium surfaces. Bio-related titanium surface modifications based on polysaccharides and synthetic polymers have been performed by physisorption and electrostatic interactions. In comparison with polylactide coatings, physisorbed alginate coatings are capable of exhibiting pronounced cell adhesion [17
- PDA units can establish a wide range of non-covalent bonds through π stacking, hydrogen bonding, and van der Waals- and hydrophobic-interactions. PDA films have been used as the anchor layers of non-fouling polymer brushes [32][33][34], substrates for cell adhesion [35][36] and as platforms for
- controlled cell adhesion [37]. The presence of amine groups in PDA has been used for functionalization with moieties for photo-induced grafting reactions [38][39]. In this work, we study the immobilization of three compounds to the titanium surface: bisphosphonate neridronate, APTES and PDA. The neridronate
A surface acoustic wave-driven micropump for particle uptake investigation under physiological flow conditions in very small volumes
Beilstein J. Nanotechnol. 2015, 6, 414–419, doi:10.3762/bjnano.6.41
- of this effect in the area of microfluidics and life science have been developed so far (see reviews [11][12]). In the past, our lab already introduced SAW devices for quantifying cell association of targeted particles [13] and cell adhesion on implant materials [14]. One of the advantages of SAW
Oxygen-plasma-modified biomimetic nanofibrous scaffolds for enhanced compatibility of cardiovascular implants
Beilstein J. Nanotechnol. 2015, 6, 254–262, doi:10.3762/bjnano.6.24
- surface hydrophilicity by forming oxygen-containing groups at the surface and thus to improve cell adhesion and proliferation. The conditions of the plasma modification were properly adjusted in order to induce the desirable chemical surface changes while maintaining surface integrity and morphology. The
- , which is more apparent in the case of the treated samples indicates the growth and proliferation of the cells in their new microenvironment. Cell adhesion and proliferation According to Figure 4c, fibroblasts seemed to be securely attached and spread on the surface, regardless of the surface treatments
- is clearly observed, covering partially the plasma-treated surface. The enhanced cell adhesion and proliferation observed in the modified systems is attributed to plasma-induced chemical alterations of the surface and is in line with the results obtained from the physicochemical characterization of
Mechanical properties of MDCK II cells exposed to gold nanorods
Beilstein J. Nanotechnol. 2015, 6, 223–231, doi:10.3762/bjnano.6.21
- signaling, for both CTAB spheres and rods, we found within 24 h after treatment a reduction of mitochondrial activity (by MTS or LDH) as well as the activation of reactive oxygen species [13][25]. Cellular mechanics plays an important role in many biological processes comprising cell adhesion, migration
Increasing throughput of AFM-based single cell adhesion measurements through multisubstrate surfaces
Beilstein J. Nanotechnol. 2015, 6, 157–166, doi:10.3762/bjnano.6.15
- cells regulate adhesion by expressing and regulating a diverse array of cell adhesion molecules on their cell surfaces. Since different cell types express distinct sets of cell adhesion molecules, substrate-specific adhesion is cell type- and condition-dependent. Single-cell force spectroscopy is used
- to quantify the contribution of cell adhesion molecules to adhesion of cells to specific substrates at both the cell and single molecule level. However, the low throughput of single-cell adhesion experiments greatly limits the number of substrates that can be examined. In order to overcome this
- limitation, segmented polydimethylsiloxane (PDMS) masks were developed, allowing the measurement of cell adhesion to multiple substrates. To verify the utility of the masks, the adhesion of four different cell lines, HeLa (Kyoto), prostate cancer (PC), mouse kidney fibroblast and MDCK, to three extracellular
Mammalian cell growth on gold nanoparticle-decorated substrates is influenced by the nanoparticle coating
Beilstein J. Nanotechnol. 2014, 5, 2479–2488, doi:10.3762/bjnano.5.257
- biocompatible polymer exhibiting one of two different end groups, resulting in a neutral or negative surface charge of the particle. Upon observation of cell growth for three days by live cell imaging using optical dark field microscopy, it was found that all particles supported cell adhesion while no directed
- cell migration and no significant particle internalization occurred. Concerning cell adhesion and spreading as compared to cell growth on bare substrates after 3 days of incubation, a reduction by 45% and 95%, respectively, for the surfactant particle coating was observed, whereas the amino-terminated
- . Live cell imaging was performed over the course of an incubation time of three days using optical dark field microscopy in order to evaluate the cell adhesion and spreading by the cell morphology. We observe an influence of the particle coating on the growth behavior with respect to the cytotoxic
Carbon-based smart nanomaterials in biomedicine and neuroengineering
Beilstein J. Nanotechnol. 2014, 5, 1849–1863, doi:10.3762/bjnano.5.196
- of consisting of a continuous rough surface, i.e., the CNTs film, which was demonstrated to improve cell adhesion. Patch-clamp electrophysiology: One of the first studies reporting on the electrical activity of in vitro neuronal networks coupled to MWCNT-substrates was that of Lovat and co-workers
- because its nanostructure and its chemical stability render it a good candidate for favouring cell adhesion. In one of the first studies, Li and co-workers [138] studied the biocompatibility of graphene and the contingent changes in the expression of the protein GAP43, which is associated with the growth
- been studied by Park et al. [144]; the results showed not only an improved differentiation but also an enhanced cell adhesion and neurites formation compared to control conditions. Moreover, the expression of laminin-related receptors and of genes involved in the calcium signalling pathway was up
Biocompatibility of cerium dioxide and silicon dioxide nanoparticles with endothelial cells
Beilstein J. Nanotechnol. 2014, 5, 1795–1807, doi:10.3762/bjnano.5.190
- investigated HUVEC populations presented an endothelial phenotype up to the highest investigated passage number as can be seen in Figure 1. All passages showed mainly CD31+ (platelet endothelial cell adhesion molecule 1 (PECAM-1)) and von Willebrand factor (vWF) positive cells (endothelial cells, Figure 1a
- (platelet endothelial cell adhesion molecule 1 (PECAM-1)). b) Proportion of CD90+ cells [%]. For purity examinations, CD90 as fibroblast cell type specific marker was determined. The investigations were performed on up to 9 passages and on HUVEC from two different suppliers (PromoCell GmbH and provitro GmbH
Near-field photochemical and radiation-induced chemical fabrication of nanopatterns of a self-assembled silane monolayer
Beilstein J. Nanotechnol. 2014, 5, 1441–1449, doi:10.3762/bjnano.5.156
- templates are found in catalysis [9], biochemical surface engineering [10], cell adhesion [11], and biomineralization [12]. It has been shown previously that chemically nanostructured surfaces can be used as platforms for the graphoepitaxial growth of block-copolymer nanostructures [13] and to control the
Influence of the PDMS substrate stiffness on the adhesion of Acanthamoeba castellanii
Beilstein J. Nanotechnol. 2014, 5, 1393–1398, doi:10.3762/bjnano.5.152
- cell adhesion area of A. castellanii trophozoites on polydimethylsiloxane (PDMS) substrates with different Young’s moduli (4 kPa, 29 kPa, and 128 kPa), we find significant differences in cell adhesion area as a function of substrate stiffness. In particular, the cell adhesion area of A. castellanii
- in the human body are the eye and the brain, i.e., very soft environments. Thus, our study provides first hints towards the relevance of mechanical aspects for the pathogenicity of eukaryotic parasites. Keywords: acanthamoeba; cell adhesion; elastic substrates; mechanosensing; silicones
- stiffness plays a decisive role for controlling cell adhesion on soft substrates, but also the specific mechanical anchorage of adhesion molecules [11]. The mechanosensory function of cells is supposed to be closely linked to the mechanisms of active force generation in cells. Analyzing cellular traction
Model systems for studying cell adhesion and biomimetic actin networks
Beilstein J. Nanotechnol. 2014, 5, 1193–1202, doi:10.3762/bjnano.5.131
- 10.3762/bjnano.5.131 Abstract Many cellular processes, such as migration, proliferation, wound healing and tumor progression are based on cell adhesion. Amongst different cell adhesion molecules, the integrin receptors play a very significant role. Over the past decades the function and signalling of
- be incorporated into lipid vesicles, too. We here review the mechanisms of integrin-mediated cell adhesion and recent advances in the field of minimal cells towards synthetic adhesion. We focus on reconstituting integrins into lipid structures for mimicking cell adhesion and on the incorporation of
- actin networks and talin into model cells. Keywords: actin network; cell adhesion; giant unilamellar vesicle; integrin; lipid bilayer; synthetic cell; protein reconstitution; talin; Review Introduction Since Hooke first described a biological cell in 1665 tremendous progress has been made in
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