Model systems for studying cell adhesion and biomimetic actin networks

• Dorothea Brüggemann,
• Johannes P. Frohnmayer and
• Joachim P. Spatz

Beilstein J. Nanotechnol. 2014, 5, 1193–1202, doi:10.3762/bjnano.5.131

• cytoskeletal assembly and membrane targeting. Further research on the influence of talin on lipid membranes was carried out by Takiguchi and co-workers, who studied the effects of adding talin to liposome solutions. They discovered a membrane-breaking function of talin: Lipid membranes were found to open

• , talin can also be a useful tool for controlled manipulation of liposome morphology, which can play an important role in the development of synthetic cells. Since the early 2000’s, research on talin reconstituted in lipid bilayers has not been pursued anymore although many fundamental results on cellular

• ) Nature Publishing Group.) Confocal fluorescence micrographs of giant actin-filled liposomes. Lipid membranes are labelled with rhodamine (red); actin is labelled with AlexaFluor 488 (green). Insets indicate the nature of the actin-membrane interaction. (A) The actin filament solution inside a liposome

Nanoscopic surfactant behavior of the porin MspA in aqueous media

• Ayomi S. Perera,
• Hongwang Wang,
• Tej B. Shrestha,
• Deryl L. Troyer and
• Stefan H. Bossmann

Beilstein J. Nanotechnol. 2013, 4, 278–284, doi:10.3762/bjnano.4.30

• ; hydrophobic interaction; liposome-type cluster; protein cluster; supramolecular; temperature influence; zeta potential; Introduction The homo-octameric porin MspA from Mycobacterium smegmatis is one of the most stable proteins known to date [1]. Due to its size and unique structure [2], its resistance to

• microscopy (TEM). The TEM were prepared by immersing carbon-coated 200-mesh copper grids in aqueous liposome-containing solutions, followed by counter-staining by 2% aqueous uranyl acetate solution, and overnight drying in a desiccator. The dried grids were analyzed by using a HRTEM FEI Tecnai F20 XT Field

Magnetic-Fe/Fe₃O₄-nanoparticle-bound SN38 as carboxylesterase-cleavable prodrug for the delivery to tumors within monocytes/macrophages

• Hongwang Wang,
• Tej B. Shrestha,
• Matthew T. Basel,
• Raj K. Dani,
• Gwi-Moon Seo,
• Sivasai Balivada,
• Marla M. Pyle,
• Heidy Prock,
• Olga B. Koper,
• Prem S. Thapa,
• David Moore,
• Ping Li,
• Viktor Chikan,
• Deryl L. Troyer and
• Stefan H. Bossmann

Beilstein J. Nanotechnol. 2012, 3, 444–455, doi:10.3762/bjnano.3.51

• tissue have been observed [22]. Liposome encapsulation of SN38 (LE-SN38) enhances the solubility of SN38 and provides protection from rapid drug degradation. Increased cytotoxicity against various tumor cell lines and better therapeutic efficacy in xenograft mouse models, as compared to CPT-11, have been

Microfluidic anodization of aluminum films for the fabrication of nanoporous lipid bilayer support structures

• Jaydeep Bhattacharya,
• Alexandre Kisner,
• Andreas Offenhäusser and
• Bernhard Wolfrum

Beilstein J. Nanotechnol. 2011, 2, 104–109, doi:10.3762/bjnano.2.12

• setup for performing biological experiments without the need to transfer the brittle nanoporous material. We demonstrate this technique by using the same microfluidic system for membrane fabrication and subsequent liposome fusion onto the nanoporous support structure. The resulting bilayer formation is