Imaging the intracellular degradation of biodegradable polymer nanoparticles

• Anne-Kathrin Barthel,
• Martin Dass,
• Melanie Dröge,
• Jens-Michael Cramer,
• Daniela Baumann,
• Markus Urban,
• Katharina Landfester,
• Volker Mailänder and
• Ingo Lieberwirth

Beilstein J. Nanotechnol. 2014, 5, 1905–1917, doi:10.3762/bjnano.5.201

• of smaller particles (40 nm) is lower than for larger ones (200 nm). However, the degradation was observed only 180 min after incubation into the cells. In general, the quantification of nanoparticle uptake and cell loading can be classified into sample-conserving and sample-destructive techniques

Biocompatibility of cerium dioxide and silicon dioxide nanoparticles with endothelial cells

• Claudia Strobel,
• Martin Förster and
• Ingrid Hilger

Beilstein J. Nanotechnol. 2014, 5, 1795–1807, doi:10.3762/bjnano.5.190

• cells. The intracellular ROS generation was not only dependent on nanoparticle size, but also on cell type due to potential differences in nanoparticle uptake and retention rates (CeO2 nanoparticles). With consideration of the expected nanoparticle concentrations in endothelial cells in vivo, the impact

Different endocytotic uptake mechanisms for nanoparticles in epithelial cells and macrophages

• Dagmar A. Kuhn,
• Dimitri Vanhecke,
• Benjamin Michen,
• Fabian Blank,
• Peter Gehr,
• Alke Petri-Fink and
• Barbara Rothen-Rutishauser

Beilstein J. Nanotechnol. 2014, 5, 1625–1636, doi:10.3762/bjnano.5.174

• mechanisms, that is, phagocytosis, macropinocytosis, clathrin- and caveolin-mediated endocytosis, were investigated using a mouse macrophage (J774A.1) and a human alveolar epithelial type II cell line (A549). In order to deduce the involved pathway in nanoparticle uptake, selected inhibitors specific for one

Precise quantification of silica and ceria nanoparticle uptake revealed by 3D fluorescence microscopy

• Adriano A. Torrano and
• Christoph Bräuchle

Beilstein J. Nanotechnol. 2014, 5, 1616–1624, doi:10.3762/bjnano.5.173

• to be accurate by independent stimulated emission depletion (STED) microscopy, a super-resolution technique [28][29]. Although developed for the absolute quantification of the nanoparticle uptake by cells, this method was made flexible to allow for the quantification in absolute and also in relative

• . In this section, we want to present in detail how Particle_in_Cell-3D was used to study the cell type-dependent uptake of 310 nm silica nanoparticles into human vascular endothelial cells (HUVEC) and cancer cells derived from the cervix carcinoma (HeLa). The nanoparticle uptake by single cells was

• intensity showed a Gaussian distribution with a mean value of 48090 pixel intensities per nanoparticle for silica particles in the cell medium for HeLa cells and 49430 pixel intensities per nanoparticle for silica particles in the cell medium for HUVEC cells. Quantification of silica-nanoparticle uptake In

The cell-type specific uptake of polymer-coated or micelle-embedded QDs and SPIOs does not provoke an acute pro-inflammatory response in the liver

• Markus Heine,
• Alexander Bartelt,
• Oliver T. Bruns,
• Denise Bargheer,
• Artur Giemsa,
• Barbara Freund,
• Ludger Scheja,
• Christian Waurisch,
• Alexander Eychmüller,
• Rudolph Reimer,
• Horst Weller,
• Peter Nielsen and
• Joerg Heeren

Beilstein J. Nanotechnol. 2014, 5, 1432–1440, doi:10.3762/bjnano.5.155

• : hepatocytes; inflammation; Kupffer cells; liver sinusoidal endothelial cells; nanoparticle toxicity; nanoparticle uptake; quantum dots; superparamagnetic iron-oxide nanocrystals; Introduction The superior optical properties of QDs compared to organic dyes render them promising candidates for the demands of

• haemolysis or nanoparticle uptake [13][14]. In most studies so far, complex surface modification was carried out to achieve water-solubility of hydrophobic QDs or SPIOs [15][16]. Another way to make nanocrystals hydrophilic is the embedding of QDs or SPIOs into the core of lipid micelles [17][18][19]. After

The protein corona protects against size- and dose-dependent toxicity of amorphous silica nanoparticles

• Dominic Docter,
• Christoph Bantz,
• Dana Westmeier,
• Hajo J. Galla,
• Qiangbin Wang,
• James C. Kirkpatrick,
• Peter Nielsen,
• Michael Maskos and
• Roland H. Stauber

Beilstein J. Nanotechnol. 2014, 5, 1380–1392, doi:10.3762/bjnano.5.151

• protein corona on the cellular uptake Nanoparticle uptake is an important determinant for nanopathology [22][34][35]. To investigate the effect of the protein corona on cellular uptake mechanisms we analyzed the uptake of two fluorescently labeled ASPs with comparable physico-chemical characteristics