Mismatch detection in DNA monolayers by atomic force microscopy and electrochemical impedance spectroscopy

• Maryse D. Nkoua Ngavouka,
• Pietro Capaldo,
• Elena Ambrosetti,
• Giacinto Scoles,
• Loredana Casalis and
• Pietro Parisse

Beilstein J. Nanotechnol. 2016, 7, 220–227, doi:10.3762/bjnano.7.20

• , the AFM tip is scanned at high load (approx. 100 nN) over the TOEG6 SAM, operating in a buffer solution (10 mM Tris-HCl, 1 mM EDTA, (hereafter TE), 1 M NaCl, pH 7.1) containing 5 μM thiolated ssDNA oligonucleotides. The applied load is sufficient to displace the TOEG6 molecules from the gold surface

• strategies based on the use of ssDNA monolayers tethered to gold substrates, for the detection of mismatches in DNA oligonucleotides. Both the strategies are label-free and are sensitive enough to detect point mutations. In Table 2 we report a comparison between the performance of our two approaches (nano

Single-molecule mechanics of protein-labelled DNA handles

• Vivek S. Jadhav,
• Dorothea Brüggemann,
• Florian Wruck and
• Martin Hegner

Beilstein J. Nanotechnol. 2016, 7, 138–148, doi:10.3762/bjnano.7.16

• reaction at 4 °C with T4 DNA ligase (Roche, Lifescience, Switzerland). Excess oligonucleotides were cleaned off by ethanol precipitation using sodium acetate as counter ions. Modified λ-DNA was resuspended in 10 mM HEPES, 1 mM EDTA buffer, pH 7.2. The biotinylated end was modified with a 100× concentration

Silica-coated upconversion lanthanide nanoparticles: The effect of crystal design on morphology, structure and optical properties

• Uliana Kostiv,
• Miroslav Šlouf,
• Hana Macková,
• Alexander Zhigunov,
• Hana Engstová,
• Katarína Smolková,
• Petr Ježek and
• Daniel Horák

Beilstein J. Nanotechnol. 2015, 6, 2290–2299, doi:10.3762/bjnano.6.235

• particles to be considered as probes of target proteins, oligonucleotides and other biomolecules in cells and tissues. DLS experiments showed that the average hydrodynamic particle size in water was large (Dh = 163–265 nm), suggesting the formation of particle aggregates. DLS provides the z-average of the

DNA–melamine hybrid molecules: from self-assembly to nanostructures

• Rina Kumari,
• Shib Shankar Banerjee,
• Anil K. Bhowmick and
• Prolay Das

Beilstein J. Nanotechnol. 2015, 6, 1432–1438, doi:10.3762/bjnano.6.148

• of using DNA–organic hybrid structures over DNA-only based nanostructures, there are challenges regarding their solubility as well as reactivity issues. In most cases, a DNA synthesizer based on phosphoramidite chemistry is used to introduce chemical modifications in the oligonucleotides [13][14][15

• Information File 1, Table S1) using a zero linker approach. The terminal phosphomonoester groups of the oligomers, which is more nucleophilic for activation than the internucleotidic phosphodiesters of oligonucleotides, were activated by EDC in the presence of imidazole as a catalyst in aqueous solution. The

• a zero linker approach with terminal phosphate as a reacting functionality, the methodology is inexpensive [27][40], does not require multiple synthetic steps and modifies the oligonucleotides with reactive functionalities. One noteworthy point about this EDC–imidazole-mediated cross coupling

Influence of gold, silver and gold–silver alloy nanoparticles on germ cell function and embryo development

• Ulrike Taylor,
• Daniela Tiedemann,
• Christoph Rehbock,
• Wilfried A. Kues,
• Stephan Barcikowski and
• Detlef Rath

Beilstein J. Nanotechnol. 2015, 6, 651–664, doi:10.3762/bjnano.6.66

• to spermatozoa, which seemed to be driven by the surface modifications. While ligand-free AuNP and gold nanoparticles conjugated to single stranded oligonucleotides (AuNP–ssO) at least attached to the sperm membrane, BSA-coating of nanoparticles prevented even such an attachment (Figure 3). The

Current state of laser synthesis of metal and alloy nanoparticles as ligand-free reference materials for nano-toxicological assays

• Christoph Rehbock,
• Jurij Jakobi,
• Lisa Gamrad,
• Selina van der Meer,
• Daniela Tiedemann,
• Ulrike Taylor,
• Wilfried Kues,
• Detlef Rath and
• Stephan Barcikowski

Beilstein J. Nanotechnol. 2014, 5, 1523–1541, doi:10.3762/bjnano.5.165

• oligonucleotides [52][86], peptides [87] and proteins [68], species native to biological systems and hence omnipresent in toxicity assays. However, in situ conjugation always entails the risk that nanoconjugates are destroyed by post irradiation [88][89][90], leading to pyrolysis products with unpredictable side

A sonochemical approach to the direct surface functionalization of superparamagnetic iron oxide nanoparticles with (3-aminopropyl)triethoxysilane

• Bashiru Kayode Sodipo and
• Azlan Abdul Aziz

Beilstein J. Nanotechnol. 2014, 5, 1472–1476, doi:10.3762/bjnano.5.160

• peptides, antibodies, oligonucleotides or polymers [7][8][9][10][11][12][13]. As illustrated in Table 1, through a conventional method APTES can be grafted onto SPION by stirring, heating or combination of both heating and stirring. The combined heating and stirring process demonstrated to be the fastest

Towards multiple readout application of plasmonic arrays

• Dana Cialla,
• Karina Weber,
• René Böhme,
• Uwe Hübner,
• Henrik Schneidewind,
• Matthias Zeisberger,
• Roland Mattheis,
• Robert Möller and
• Jürgen Popp

Beilstein J. Nanotechnol. 2011, 2, 501–508, doi:10.3762/bjnano.2.54

• array define its optical properties. In order to illustrate potential applications of these plasmonic arrays for bioanalytics, the arrays were tested for DNA detection. Therefore, capture oligonucleotides were immobilized on the chemically modified quartz surface between the gold nanorhombs. The

Magnetic nanoparticles for biomedical NMR-based diagnostics

• Huilin Shao,
• Tae-Jong Yoon,
• Monty Liong,
• Ralph Weissleder and
• Hakho Lee

Beilstein J. Nanotechnol. 2010, 1, 142–154, doi:10.3762/bjnano.1.17

• assays can take two forms. For small molecular targets with sizes less than or comparable to that of the MNPs, MRSw assays can be used effectively for their detection and quantification. Small molecular analytes, such as drugs, metabolites, oligonucleotides, and proteins, can cross-link MNPs to promote