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Search for "protein corona" in Full Text gives 57 result(s) in Beilstein Journal of Nanotechnology.
Using gold nanoparticles to detect single-nucleotide polymorphisms: toward liquid biopsy
Beilstein J. Nanotechnol. 2020, 11, 263–284, doi:10.3762/bjnano.11.20
- . Specifically, the recently proposed protein corona sensor arrays in which the composition of protein corona reflects the presence of a given cancer enabled new venues in detecting diseases directly from a blood sample [140]. Moreover, the simultaneous detection of genetic mutations and disease-specific
- ]. Several limitations need to be addressed as well. One of the known issues in colloidal biosensing is the spontaneous formation of a protein corona on the surface of the particles in physiological media [146], inhibiting the interaction of the biomarkers with the colloid, thereby altering the sensitivity
Rational design of block copolymer self-assemblies in photodynamic therapy
Beilstein J. Nanotechnol. 2020, 11, 180–212, doi:10.3762/bjnano.11.15
Bombesin receptor-targeted liposomes for enhanced delivery to lung cancer cells
Beilstein J. Nanotechnol. 2019, 10, 2553–2562, doi:10.3762/bjnano.10.246
- polydisperse proteins of different sizes [27]. Indeed, the surface properties of various nanoparticles have been shown to change dramatically in the presence of plasma or serum [28] with the establishment of an adsorbed protein corona around the nanoparticle. It is now widely accepted that the particle protein
- corona presents a new biomolecular interface that underpins the dynamic interactions of nanosystems and their biological targets. The presentation of a high affinity GRPR antagonist peptide on the liposomal surface is expected to maintain liposomal cell-binding affinity by virtue of its high affinity for
Engineered superparamagnetic iron oxide nanoparticles (SPIONs) for dual-modality imaging of intracranial glioblastoma via EGFRvIII targeting
Beilstein J. Nanotechnol. 2019, 10, 1860–1872, doi:10.3762/bjnano.10.181
- proteins, resulting in the formation of protein corona [48][49]. To minimize the adverse effects of the presence of the protein corona in vivo, the surface coating using PEG can endow the NPs with so-called “stealth” properties to reduce the adsorption of high molecular weight proteins, allowing them to
Serum type and concentration both affect the protein-corona composition of PLGA nanoparticles
Beilstein J. Nanotechnol. 2019, 10, 1002–1015, doi:10.3762/bjnano.10.101
- /bjnano.10.101 Abstract Background: When nanoparticles (NPs) are applied into a biological fluid, such as blood, proteins bind rapidly to their surface forming a so-called “protein corona”. These proteins are strongly attached to the NP surface and confers them a new biological identity that is crucial
- for the biological response in terms of body biodistribution, cellular uptake, and toxicity. The corona is dynamic in nature and it is well known that the composition varies in dependence of the physicochemical properties of the NPs. In the present study we investigated the protein corona that forms
- assay, zeta potential measurements, and liquid chromatography–mass spectrometry/mass spectrometry (LC–MS/MS). Additionally, the time-dependent cell interaction of PLGA NPs in the absence or presence of a preformed protein corona was assessed by in vitro incubation experiments with the human liver cancer
Engineering of oriented carbon nanotubes in composite materials
Beilstein J. Nanotechnol. 2018, 9, 415–435, doi:10.3762/bjnano.9.41
- the CNT diameter and length are critical parameters in protein corona formation and biocompatibility [11][12]. In another research investigating the effect of aligning CNTs in composite material structures, a remarkable improvement in the electrical properties of CNT composites was observed, as
Methionine-mediated synthesis of magnetic nanoparticles and functionalization with gold quantum dots for theranostic applications
Beilstein J. Nanotechnol. 2017, 8, 1734–1741, doi:10.3762/bjnano.8.174
- have a dramatic effect on the formation of the surface protein corona in the bloodstream that affects CoFe2O4@Met–Au NPs passive targeting and uptake into tumor cells. The elaborated functionalization of magnetic NPs with gold QDs represents a promising multi-task platform for linking magnetic NPs with
Bright fluorescent silica-nanoparticle probes for high-resolution STED and confocal microscopy
Beilstein J. Nanotechnol. 2017, 8, 1283–1296, doi:10.3762/bjnano.8.130
- amounts of salts, amino acids and proteins. In aqueous media, electrostatic repulsion contributes to particle stabilisation. In presence of salts at neutral pH values, this type of stabilisation might be ineffective. In contrast, the formation of a protein corona at the interface between particle surface
Evaluation of quantum dot conjugated antibodies for immunofluorescent labelling of cellular targets
Beilstein J. Nanotechnol. 2017, 8, 1238–1249, doi:10.3762/bjnano.8.125
- between Qdots and functional antibodies, the overall surface charge of Qdot-Ab conjugates, and whether there is a presence of a protein corona. The labelling ratio of Qdots to antibodies present within commercially available Qdot-Ab conjugates has been previously evaluated, where the non-specific
- protein adsorption and thus the formation of a protein corona [38]. Qdots with a zwitterionic surface have a zeta potential of near zero, are resistant to non-specific binding onto cells, and have a high colloidal stability [39]. Commercially available Qdot-Abs evaluated in this report were used in fixed
Nano-engineered skin mesenchymal stem cells: potential vehicles for tumour-targeted quantum-dot delivery
Beilstein J. Nanotechnol. 2017, 8, 1218–1230, doi:10.3762/bjnano.8.123
- , which could be a desirable event in targeted tumour therapy. The optimal uptake conditions for NPs could depend on the particle size, surface modifications, protein corona, and recipient cell line. Previous studies have suggested the incubation of NIH3T3 mouse fibroblasts with 16 nM QDs for 6 h as the
- varies depending on the cell and particle type. One of the factors affecting uptake is the protein corona that forms around NPs in serum-containing medium. Protein aggregates decrease gold NP uptake depending on size and cell type [41]. In the present study, we analysed the QD uptake pathways under both
- QDs possessing a protein corona are differently recognized by NIH3T3 cells and internalized by different pathways [23], consistent with the data from the present study. Interestingly, MSCs showed more effective internalization of QDs under serum-free conditions, as the protein corona interferes with
Uptake of the proteins HTRA1 and HTRA2 by cells mediated by calcium phosphate nanoparticles
Beilstein J. Nanotechnol. 2017, 8, 381–393, doi:10.3762/bjnano.8.40
- dispersion in biological media containing proteins (like RPMI/10% FCS) is much better due to the formation of a protein corona [48][49]. The concentration of the labelled proteins was determined by UV–vis spectroscopy (Table 2). Table 3 gives the quantification data of the functionalized nanoparticles, where
- also influence their uptake [64][65][66][67]. Krais et al. have shown that serum proteins were necessary for cancer cells to take up folic acid-conjugated iron oxide nanoparticles [68]. The nature of the protein corona on the protein-loaded calcium phosphate nanoparticles after immersion in cell
On the pathway of cellular uptake: new insight into the interaction between the cell membrane and very small nanoparticles
Beilstein J. Nanotechnol. 2016, 7, 1296–1311, doi:10.3762/bjnano.7.121
- dependent on particle size and the serum content in the culture medium [11][12]. In serum-free conditions the uptake of SiNPs has been determined to be much higher than in the presence of fetal calf serum (FCS), which is attributed to the fact that SiNPs build up a protein corona and tend to agglomerate in
Surface coating affects behavior of metallic nanoparticles in a biological environment
Beilstein J. Nanotechnol. 2016, 7, 246–262, doi:10.3762/bjnano.7.23
- may serve as desirable barriers preventing NPs from agglomeration in biomedical applications [18]. Moreover, when NPs enter a biological fluid, electrostatic, dispersive, and covalent interactions cause proteins to adsorb on NP surfaces, leading to the formation of a dynamic protein corona [30][36][37
- uptake and toxicity of metallic NPs [41][42], whereas differences in surface coatings influence cytotoxicity and surface charge [43]. However, it is still unclear how different surface coatings affect the interaction of NPs with biological environments and the formation of the protein corona. Because
- evaluation of metallic NPs in biological environments. Effect of albumin on the dispersibility of AgNPs and SPIONs in biological media When suspended in biological fluids, NPs rapidly interact with proteins that form a dynamical layer all over the NP surface, known as a protein corona (PC) [36][37][39
The eNanoMapper database for nanomaterial safety information
Beilstein J. Nanotechnol. 2015, 6, 1609–1634, doi:10.3762/bjnano.6.165
- templates (e.g., Protein Corona CSV files or ModNanoTox Excel files), and custom formats, provided by partners (e.g., the NanoWiki RDF dump [27]). ISA-Tab [15] files are converted by compressing the chain of protocols into a single entry, yet retaining all the protocol parameters and recording the material
- allowing parsing of the entire ModNanoTox complex organisation into the internal eNanoMapper data model. Protein Corona The demonstration data set, extracted from [28], focuses on the biological identity of ENMs. The authors used the composition of the protein corona “fingerprint” to predict the cell
- . Among the factors in play in protein corona, biological interaction was chosen to be represented by cell association because of its relevance to biodistribution, inflammatory response potential, and in vivo toxicity. The eNanoMapper prototype described in this paper is able to capture this protein
Protein corona – from molecular adsorption to physiological complexity
Beilstein J. Nanotechnol. 2015, 6, 857–873, doi:10.3762/bjnano.6.88
- Hospital of Mainz, Langenbeckstrasse 1, 55101 Mainz, Germany 10.3762/bjnano.6.88 Abstract In biological environments, nanoparticles are enshrouded by a layer of biomolecules, predominantly proteins, mediating its subsequent interactions with cells. Detecting this protein corona, understanding its
- formation with regards to nanoparticle (NP) and protein properties, and elucidating its biological implications were central aims of bio-related nano-research throughout the past years. Here, we discuss the mechanistic parameters that are involved in the protein corona formation and the consequences of this
- involvement of Coulomb-type interactions between NPs and charged patches on the protein surface. Moreover, we discuss novel aspects related to the complexity of the protein corona forming under physiological conditions in full serum. Specifically, we address the relation between particle size and corona
Biological responses to nanoscale particles
Beilstein J. Nanotechnol. 2015, 6, 380–382, doi:10.3762/bjnano.6.37
- , their surface-active components (i.e., proteins) will adhere to the nanoparticles and form a so-called “protein corona”. As a result, biological systems never come into contact with bare nanoparticles, but rather with their protein-coated analogues. Since this discovery, a large variety of experimental
- preferred as a function of shape, size, and most importantly, surface functionalization and protein corona, could thus far not be established. The SPP1313 research network resulted from an open national call after an intensive evaluation of proposals by an international group of experts. It operated for a
Overview about the localization of nanoparticles in tissue and cellular context by different imaging techniques
Beilstein J. Nanotechnol. 2015, 6, 263–280, doi:10.3762/bjnano.6.25
- their toxicity is of prime interest in the safety evaluation of NP. Importantly, for a precise understanding of the biological and toxicological effects of complex NP it is important to understand which part of the NP induces the observed effects, e.g., the inorganic core, the ligand shell, the protein
- corona or even the drug or label inside or associated with the particle. Therefore, more sophisticated imaging methods are needed that allow one to distinguish different parts of a NP within tissues or cells. Furthermore, the expertise of specially trained toxicological pathologists is essential in any
The distribution and degradation of radiolabeled superparamagnetic iron oxide nanoparticles and quantum dots in mice
Beilstein J. Nanotechnol. 2015, 6, 111–123, doi:10.3762/bjnano.6.11
- to play important roles in endocytosing processes and are known to be among the most prominent cell types which take up protein-corona-covered nanoparticles [22][40]. The confocal images of the cryosections exhibit the cell distribution within the liver and confirm this hypothesis. Internalized Qdots
The fate of a designed protein corona on nanoparticles in vitro and in vivo
Beilstein J. Nanotechnol. 2015, 6, 36–46, doi:10.3762/bjnano.6.5
- back into the plasma and into tissue. This study confirms that adsorbed transferrin from a preformed protein corona is efficiently taken up by cells. It is also highlighted that a radiolabelling technique described in this study may be of value to investigate the role of protein corona formation in
- vivo for the respective nanoparticle uptake. Keywords: albumin; 59Fe; 125I; organ uptake; protein corona; SPIOs; transferrin; Introduction Nanoparticles (NPs) have unique capabilities to interact with cells and organs which mark them as attractive working material in nanobioscience and nanomedicine
- with biological matrices such as the blood, are immediately coated by a layer of proteins, resulting in a so called „protein corona“ [8][9][10][11][12][13][14]. The protein corona modifies and shields the surface of the xenobiotic particles and may subsequently influence or even determine their
Nanoparticle interactions with live cells: Quantitative fluorescence microscopy of nanoparticle size effects
Beilstein J. Nanotechnol. 2014, 5, 2388–2397, doi:10.3762/bjnano.5.248
- machinery. An interesting aspect in this process is that, in the biological milieu, the NPs typically adsorb dissolved biomolecules, so that they are enshrouded by a so-called ‘protein corona’ [6][7][8]. NPs interact with cells via this layer of biomolecules, at least during the initial encounter, so that
- [24][25][26][27]. Because of the inevitable protein corona formation in any biological environment, one has also to be aware that experiments on cultured cells may yield results different from in vivo studies. Over the past few years, we have used spinning disk confocal fluorescence microscopy to
- cells and the bare NP surfaces rather than NPs carrying a protein corona of unknown composition. By means of inhibitory drugs that specifically interfere with the one or the other endocytosis pathway, the endocytosis pathways involved in the uptake of small and large NPs have been revealed. Finally
Inorganic Janus particles for biomedical applications
Beilstein J. Nanotechnol. 2014, 5, 2346–2362, doi:10.3762/bjnano.5.244
- activity, the magnetic properties, as well as their biocompatibility and interaction with human blood serum. Keywords: bioimaging (CT; MRI; Multi-photon); hetero-nanoparticles; Janus particles; protein corona; synthesis; Introduction In the recent years, there has been an increasing interest in design
- nanoparticles, while the composition of the protein corona is responsible for the fate of the nanoparticles in the organism. Up to now, the formation and the composition of the protein corona is far from being completely understood, but the importance is obvious for any further application of nanoparticles in
- theranostics [113]. The analysis of the protein corona of nanoparticles shows that the binding profiles do not reflect the relative protein concentrations of the plasma. Recently, Tenzer et al. showed that there is no direct correlation of the surface charge and the isoelectric point of proteins enriched in
Coating with luminal gut-constituents alters adherence of nanoparticles to intestinal epithelial cells
Beilstein J. Nanotechnol. 2014, 5, 2308–2315, doi:10.3762/bjnano.5.239
- conceivable increase in particle size, the modification of the particle surface with a protein corona will influence the interaction of NPs with the intestinal mucosal defense system - a multifactorial barrier against mucosal intruders, composed of, e.g., enzymes, soluble surrogate receptors, immunoglobulins
- particle adherence to the cells. The potential consequences of the presence of proteins in biological systems on the behaviour of NPs in that environment are gaining increasing interest. Indeed, the formation of a protein corona around NPs was reviewed in several articles in the last years [22][23][24
- ], and possible implications for the particle–cell interactions are considered. For example, it was shown that human serum albumin (HSA), BSA and fetal bovine serum can build a protein corona around NPs whereby the particles are stabilized against agglomeration and the colloid stability of the particle
Effects of surface functionalization on the adsorption of human serum albumin onto nanoparticles – a fluorescence correlation spectroscopy study
Beilstein J. Nanotechnol. 2014, 5, 2036–2047, doi:10.3762/bjnano.5.212
- , independent of their surface charge. The differences in the thickness of the protein corona were rationalized in terms of the different orientations in which HSA adsorbs onto the NPs. The midpoints of the binding transition, which quantifies the affinity of HSA toward the NP, were observed to differ by almost
- four orders of magnitude. These variations can be understood in terms of specific Coulombic interactions between the proteins and the NP surfaces. Keywords: fluorescence correlation spectroscopy; human serum albumin; nanoparticles; protein corona; quantum dots; Introduction In recent years, both
- contact with extracellular fluids such as blood plasma or lung epithelial lining fluid, which contain a huge variety of dissolved biomolecules including lipids and proteins. These can adsorb onto the NP surface and completely enshroud the NP, forming the so-called “protein corona” [11][12][13][14][15
PVP-coated, negatively charged silver nanoparticles: A multi-center study of their physicochemical characteristics, cell culture and in vivo experiments
Beilstein J. Nanotechnol. 2014, 5, 1944–1965, doi:10.3762/bjnano.5.205
- biological media (i.e., in the presence of proteins) the surface of silver nanoparticles is rapidly coated by a protein corona that influences their physicochemical and biological properties including cellular uptake. Silver nanoparticles are taken up by cell-type specific endocytosis pathways as
- down the release of the toxic silver ions. The formation of nanoscopic silver chloride may also be responsible for the cytotoxicity of silver [44]. The protein corona around silver nanoparticles It is now well accepted that nanoparticles acquire a protein corona after contact with biological media [45
- adsorption onto nanoparticle surfaces is a critical step towards understanding the formation of the protein corona at the full complexity of the physiological situation [45][54][55][56][57][58]. We have investigated the formation of a protein corona of serum albumin around silver nanoparticles [56
The surface properties of nanoparticles determine the agglomeration state and the size of the particles under physiological conditions
Beilstein J. Nanotechnol. 2014, 5, 1774–1786, doi:10.3762/bjnano.5.188
- presence of proteins [11] because NPs tend to form a protein corona [12][13] that is considered to mediate cellular responses [14] and uptake pathways [15][16]. Depending on the nature of colloidal stabilization, the formation of a protein corona and/or even the conditions of physiological salinity can
- the protein corona. Nevertheless, the use of this data for size determination would result in arbitrary results due to the fact that the surface properties are altered by protein adsorption. Additionally, a broad fraction was detected eluting at later elution volumes. Since in AF-FFF a sample is
- values are given in brackets since a loss of material due to the filtration process is likely (especially for the quaternized samples). Although the addition of serum proteins and the subsequent formation of a protein corona leads to an increase in colloidal stability of the electrostatically stabilized
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