Overview about the localization of nanoparticles in tissue and cellular context by different imaging techniques

• Anja Ostrowski,
• Daniel Nordmeyer,
• Alexander Boreham,
• Cornelia Holzhausen,
• Lars Mundhenk,
• Christina Graf,
• Martina C. Meinke,
• Annika Vogt,
• Sabrina Hadam,
• Jürgen Lademann,
• Eckart Rühl,
• Ulrike Alexiev and
• Achim D. Gruber

Beilstein J. Nanotechnol. 2015, 6, 263–280, doi:10.3762/bjnano.6.25

• multidisciplinary team involved in the development, characterization, and risk assessment of novel NP [20]. For this purpose, professional training and standardized certification of toxicological pathologists are firmly established, for example, by the European or American Colleges of Veterinary Pathologists [21

• structured illumination, Raman microspectroscopy and X-ray microscopy. Review Light microscopy Light microscopic examination has become the gold standard of pathologic evaluation and risk assessment of drugs and nanoparticles during the past 50 years. This technology uses sections of 3 to 8 µm thickness from

• used for the concomitant localization of NP in cells [183]. Conclusion A toxicopathological evaluation of tissues is indispensable for any risk assessment and hazard identification of NP [20]. Each of the microscopic techniques reviewed here has its advantages and limitations, as summarized in Table 1

Functionalized polystyrene nanoparticles as a platform for studying bio–nano interactions

• Cornelia Loos,
• Tatiana Syrovets,
• Anna Musyanovych,
• Volker Mailänder,
• Katharina Landfester,
• G. Ulrich Nienhaus and
• Thomas Simmet

Beilstein J. Nanotechnol. 2014, 5, 2403–2412, doi:10.3762/bjnano.5.250

• Harvard Center for Risk Assessment concluded that there is no reason for concern from exposure to polystyrene materials used in food-contact applications [16]. Due to its inertness and biocompatibility, polystyrene together with polycarbonate is widely used for the production of biomedical devices and

PVP-coated, negatively charged silver nanoparticles: A multi-center study of their physicochemical characteristics, cell culture and in vivo experiments

• Sebastian Ahlberg,
• Alexandra Antonopulos,
• Jörg Diendorf,
• Ralf Dringen,
• Matthias Epple,
• Rebekka Flöck,
• Wolfgang Goedecke,
• Christina Graf,
• Nadine Haberl,
• Jens Helmlinger,
• Fabian Herzog,
• Frederike Heuer,
• Stephanie Hirn,
• Christian Johannes,
• Stefanie Kittler,
• Manfred Köller,
• Katrin Korn,
• Wolfgang G. Kreyling,
• Fritz Krombach,
• Jürgen Lademann,
• Kateryna Loza,
• Eva M. Luther,
• Marcelina Malissek,
• Martina C. Meinke,
• Daniel Nordmeyer,
• Anne Pailliart,
• Jörg Raabe,
• Fiorenza Rancan,
• Barbara Rothen-Rutishauser,
• Eckart Rühl,
• Carsten Schleh,
• Andreas Seibel,
• Christina Sengstock,
• Lennart Treuel,
• Annika Vogt,
• Katrin Weber and
• Reinhard Zellner

Beilstein J. Nanotechnol. 2014, 5, 1944–1965, doi:10.3762/bjnano.5.205

Precise quantification of silica and ceria nanoparticle uptake revealed by 3D fluorescence microscopy

• Adriano A. Torrano and
• Christoph Bräuchle

Beilstein J. Nanotechnol. 2014, 5, 1616–1624, doi:10.3762/bjnano.5.173

• medical treatment, and also for a correct risk assessment of nanoparticles. In both cases, knowledge regarding the kinetics of particle internalization gives the dose as a function of the time and allows for the investigation of a variety of parameters on that might influence the uptake behavior. Typical

• nanoparticles, possibly influenced by cell division. Particle_in_ell-3D can be applied to investigate the dose-dependent effects for the risk assessment of nanoparticles. Additionally, this method can be used to study which factors are determinant for the successful attachment, internalization and cargo release

Current state of laser synthesis of metal and alloy nanoparticles as ligand-free reference materials for nano-toxicological assays

• Christoph Rehbock,
• Jurij Jakobi,
• Lisa Gamrad,
• Selina van der Meer,
• Daniela Tiedemann,
• Ulrike Taylor,
• Wilfried Kues,
• Detlef Rath and
• Stephan Barcikowski

Beilstein J. Nanotechnol. 2014, 5, 1523–1541, doi:10.3762/bjnano.5.165

• [162], Ag and Au [163] nanoparticles in in vivo trials on rats. Details related to all the above mentioned studies are summarized in Table 2. Conclusion Risk assessment in medical implant approval requires the quantification of unintended effects related to wear debris on the nanoscale. Hence, suitable

Mimicking exposures to acute and lifetime concentrations of inhaled silver nanoparticles by two different in vitro approaches

• Fabian Herzog,
• Kateryna Loza,
• Sandor Balog,
• Martin J. D. Clift,
• Matthias Epple,
• Peter Gehr,
• Alke Petri-Fink and
• Barbara Rothen-Rutishauser

Beilstein J. Nanotechnol. 2014, 5, 1357–1370, doi:10.3762/bjnano.5.149

• , an expression of SOD1 and HMOX1 was not induced. Discussion There is an urgent need for realistic exposure scenarios for in vitro toxicity testing of nanomaterials. We considered in this study the three main important points for inhalation risk assessment studies: realistic NP application simulating