Cationic PEGylated polycaprolactone nanoparticles carrying post-operation docetaxel for glioma treatment

• Cem Varan and
• Erem Bilensoy

Beilstein J. Nanotechnol. 2017, 8, 1446–1456, doi:10.3762/bjnano.8.144

• increased the particle size and decreased the cellular uptake of nanoparticles [66]. Cationic core–shell nanoparticles are also quite suitable for the delivery of negatively charged gene and drug to tumor tissue. Wei et al. used cationic core–shell nanoparticles for the active targeted delivery of siRNA on

Uptake of the proteins HTRA1 and HTRA2 by cells mediated by calcium phosphate nanoparticles

• Olga Rotan,
• Katharina N. Severin,
• Simon Pöpsel,
• Alexander Peetsch,
• Melisa Merdanovic,
• Michael Ehrmann and
• Matthias Epple

Beilstein J. Nanotechnol. 2017, 8, 381–393, doi:10.3762/bjnano.8.40

• ]. For instance, nucleic acids like DNA [10][11][12][13], siRNA [14][15][16][17] and µRNA [18] have been successfully introduced to perform transfection, gene silencing, prophylactic and therapeutic vaccination [19][20][21]. All kinds of synthetic molecules and biomolecules can be transported across the

High antiviral effect of TiO₂·PL–DNA nanocomposites targeted to conservative regions of (−)RNA and (+)RNA of influenza A virus in cell culture

• Asya S. Levina,
• Marina N. Repkova,
• Elena V. Bessudnova,
• Ekaterina I. Filippova,
• Natalia A. Mazurkova and
• Valentina F. Zarytova

Beilstein J. Nanotechnol. 2016, 7, 1166–1173, doi:10.3762/bjnano.7.108

• ’-terminal region of (−)RNA [10][11] were the most chosen regions of IAV segment 5 to be affected by oligonucleotides and their analogs. The coding region close to the stop codon was often used as the target for the action of siRNA [9][12][15][16]. The other regions of the NP gene were also studied as

Hematopoietic and mesenchymal stem cells: polymeric nanoparticle uptake and lineage differentiation

• Ivonne Brüstle,
• Thomas Simmet,
• Gerd Ulrich Nienhaus,
• Katharina Landfester and
• Volker Mailänder

Beilstein J. Nanotechnol. 2015, 6, 383–395, doi:10.3762/bjnano.6.38

• granulopoetic markers was significantly suppressed. Discussion Although nanoparticles are proposed as useful drug and DNA or siRNA delivery vehicles (which could potentially change the fate of stem cells), they could also influence the cellular fate of stem cells with a potentially devastating effect. While

Comparative evaluation of the impact on endothelial cells induced by different nanoparticle structures and functionalization

• Lisa Landgraf,
• Ines Müller,
• Peter Ernst,
• Miriam Schäfer,
• Christina Rosman,
• Isabel Schick,
• Oskar Köhler,
• Hartmut Oehring,
• Vladimir V. Breus,
• Thomas Basché,
• Carsten Sönnichsen,
• Wolfgang Tremel and
• Ingrid Hilger

Beilstein J. Nanotechnol. 2015, 6, 300–312, doi:10.3762/bjnano.6.28

• ; Introduction To advance the field of nanomedicine, innovative nanoparticle formulations with suitable properties for diagnostic imaging, therapy (e.g., magnetic hyperthermia), delivery of drugs and siRNA have been developed. Apart from their feasibility for the respective application many of these

Caveolin-1 and CDC42 mediated endocytosis of silica-coated iron oxide nanoparticles in HeLa cells

• Nils Bohmer and
• Andreas Jordan

Beilstein J. Nanotechnol. 2015, 6, 167–176, doi:10.3762/bjnano.6.16

• and surface charge, were tested in HeLa cells as a model cell line. To elucidate, which molecular pathways are involved in their endocytosis, well-known endocytotic mechanisms [26][27][28] were inhibited by specific knockdown of key proteins via siRNA (Figure 1). Experimental Superparamagnetic iron

• Technologies. To achieve the optimal transfection efficiency, two transfection rounds on day 1 and 3 after cell plating were performed. In preliminary experiments the knockdown technique was optimized to cause no cell death by applying different ranges of transfection reagent with different amounts of siRNA

• were transfected according to the protocol “Thermo Scientific DharmaFECT Transfection Reagents - siRNA Transfection Protocol“ and DharmaFECT 1 siRNA Transfection Reagent (Thermo Fisher Scientific, Cat. No. T-2001-01) was used. Information about the siRNA-mix (SMARTpool®) used is shown in Table 3

Anticancer efficacy of a supramolecular complex of a 2-diethylaminoethyl–dextran–MMA graft copolymer and paclitaxel used as an artificial enzyme

• Yasuhiko Onishi,
• Yuki Eshita,
• Rui-Cheng Ji,
• Masayasu Onishi,
• Takashi Kobayashi,
• Masaaki Mizuno,
• Jun Yoshida and
• Naoji Kubota

Beilstein J. Nanotechnol. 2014, 5, 2293–2307, doi:10.3762/bjnano.5.238

• structure is stabilized at less than the critical micelle concentration. It was reported recently that the administration of DDMC as an aerosol [25] and the use of DDMC/p53 in intratumoral gene therapy [26] were effective. DDMC is very suitable for experiments using targeted siRNA or miRNA transfection in

In vitro interaction of colloidal nanoparticles with mammalian cells: What have we learned thus far?

• Moritz Nazarenus,
• Qian Zhang,
• Mahmoud G. Soliman,
• Pablo del Pino,
• Beatriz Pelaz,
• Susana Carregal-Romero,
• Joanna Rejman,
• Barbara Rothen-Rutishauser,
• Martin J. D. Clift,
• Reinhard Zellner,
• G. Ulrich Nienhaus,
• James B. Delehanty,
• Igor L. Medintz and
• Wolfgang J. Parak

Beilstein J. Nanotechnol. 2014, 5, 1477–1490, doi:10.3762/bjnano.5.161

• case of positively charged NPs adsorb to the NPs. As a consequence of counter ion adsorption the local ion concentration around the NPs surface is different from the bulk [54][87][106][107]. ii) Also nucleic acids, such as mRNA or siRNA, which are negatively charged due to their phosphate groups attach

Molecular biology approaches in bioadhesion research

• Marcelo Rodrigues,
• Birgit Lengerer,
• Thomas Ostermann and
• Peter Ladurner

Beilstein J. Nanotechnol. 2014, 5, 983–993, doi:10.3762/bjnano.5.112

• fragments of 21–22 bp in length. These short fragments are known as siRNAs. Each siRNA is unwound into two single stranded components: The passenger strand, which is degraded, and the guide strand which is recruited by the RNAi-induced silencing complex (RISC). When the guide strand fits to a given