The effect of surface charge on nonspecific uptake and cytotoxicity of CdSe/ZnS core/shell quantum dots

• Vladimir V. Breus,
• Anna Pietuch,
• Marco Tarantola,
• Thomas Basché and
• Andreas Janshoff

Beilstein J. Nanotechnol. 2015, 6, 281–292, doi:10.3762/bjnano.6.26

• biotinylated QDs [30], or to observe the movement of single, streptavidin-coated QDs along microtubules [31]. Results and Discussion ECIS and the MTS assay were used to evaluate the viability of MDCKII cells exposed to CdSe/ZnS QDs functionalized with positively-charged CA ligands, negatively-charged DHLA- or

Functionalization of α-synuclein fibrils

• Simona Povilonienė,
• Vida Časaitė,
• Virginijus Bukauskas,
• Arūnas Šetkus,
• Juozas Staniulis and
• Rolandas Meškys

Beilstein J. Nanotechnol. 2015, 6, 124–133, doi:10.3762/bjnano.6.12

• attach to one fibril, when two neutravidin molecules that are present on the surface of gold nanoparticle bind to biotin moieties on the same fibril. The application of the biotin–streptavidin (avidin) interaction, which is known to be one of the strongest interactions in nature, offers a broad range of

• possibilities with respect to functionalization of fibrils, since many streptavidin-conjugated components (enzymes and metal nanoparticles) are already available. Depending on the final aim, two strategies for functionalization may be used since the modifications can be performed before or after fibrillation

Coating with luminal gut-constituents alters adherence of nanoparticles to intestinal epithelial cells

• Heike Sinnecker,
• Katrin Ramaker and
• Andreas Frey

Beilstein J. Nanotechnol. 2014, 5, 2308–2315, doi:10.3762/bjnano.5.239

• . The biotinylated lectin RCA I was used in a concentration of 20 µg/mL in 0.2% gelatin/PBS to label the cells over night at 4 °C. Afterwards the cells were incubated with Cy5-conjugated streptavidin (GE Healthcare, Brand Amersham, Pittsburgh, USA), 5 µg/mL in 0.2% gelatin/PBS, for 90 min at rt. Finally

Model systems for studying cell adhesion and biomimetic actin networks

• Dorothea Brüggemann,
• Johannes P. Frohnmayer and
• Joachim P. Spatz

Beilstein J. Nanotechnol. 2014, 5, 1193–1202, doi:10.3762/bjnano.5.131

• membrane by biotin-streptavidin linkages. This anchorage resulted in the formation of a cortex-like actin structure within the GUVs. However, the GUVs in this study were contaminated by agarose, which adhered to the lipid membrane. A very similar approach for growing GUVs with embedded biomolecules was

• with an inert membrane (containing PEG-lipids) is homogeneous and displays a depletion zone underneath the membrane. (B) Liposomes, which contain biotinylated lipids, encapsulate networks of biotinylated actin filaments that are coupled to the membrane via biotin–streptavidin bonds. The scale bars

The morphology of silver nanoparticles prepared by enzyme-induced reduction

• Henrik Schneidewind,
• Thomas Schüler,
• Katharina K. Strelau,
• Karina Weber,
• Dana Cialla,
• Marco Diegel,
• Roland Mattheis,
• Andreas Berger,
• Robert Möller and
• Jürgen Popp

Beilstein J. Nanotechnol. 2012, 3, 404–414, doi:10.3762/bjnano.3.47

• streptavidin, a streptavidin horseradish peroxidase conjugate complex is bound via the linker molecule to the surface. In order to remove the remaining unbound peroxidase complex, the samples were washed six times in PBST buffer for 2 min each after 1 h incubation of the surface with the peroxidase complex