The cell-type specific uptake of polymer-coated or micelle-embedded QDs and SPIOs does not provoke an acute pro-inflammatory response in the liver

• Markus Heine,
• Alexander Bartelt,
• Oliver T. Bruns,
• Denise Bargheer,
• Artur Giemsa,
• Barbara Freund,
• Ludger Scheja,
• Christian Waurisch,
• Alexander Eychmüller,
• Rudolph Reimer,
• Horst Weller,
• Peter Nielsen and
• Joerg Heeren

Beilstein J. Nanotechnol. 2014, 5, 1432–1440, doi:10.3762/bjnano.5.155

• type mice, we show that 30 min after injection polymer-coated nanocrystals are primarily taken up by liver sinusoidal endothelial cells. In contrast, by using wild type, Ldlr-/- as well as Apoe-/- mice we show that nanocrystals embedded within lipid micelles are internalized by Kupffer cells and, in a

• pro-inflammatory pathways. In conclusion, internalized nanocrystals at least in mouse liver cells, namely endothelial cells, Kupffer cells and hepatocytes are at least not acutely associated with potential adverse side effects, underlining their potential for biomedical applications. Keywords

• : hepatocytes; inflammation; Kupffer cells; liver sinusoidal endothelial cells; nanoparticle toxicity; nanoparticle uptake; quantum dots; superparamagnetic iron-oxide nanocrystals; Introduction The superior optical properties of QDs compared to organic dyes render them promising candidates for the demands of

Antimicrobial nanospheres thin coatings prepared by advanced pulsed laser technique

• Alina Maria Holban,
• Valentina Grumezescu,
• Alexandru Mihai Grumezescu,
• Bogdan Ştefan Vasile,
• Roxana Truşcă,
• Rodica Cristescu,
• Gabriel Socol and
• Florin Iordache

Beilstein J. Nanotechnol. 2014, 5, 872–880, doi:10.3762/bjnano.5.99

• ) bacteria strains. Moreover, the obtained nano-coatings showed a good biocompatibility and facilitated the normal development of human endothelial cells. These nanosystems may be used as efficient alternatives in treating and preventing bacterial infections. Keywords: antimicrobial; chitosan; magnetite

• secondary electron beams with energies of 30 keV on samples covered with a thin gold layer. Cell viability Human endothelial cells (EAhy926 cell line, ATCC, USA) were grown in Dulbecco's Modified Eagle Medium (DMEM) culture medium containing 10% Fetal Bovine Serum (FBS), and 1% penicillin and neomycin

• (Sigma Aldrich, St. Louis, MO, USA). For cell proliferation and viability CellTiter96 Non-Radioactive Cell Proliferation Assay, (Promega, Madison, USA) was used. Endothelial cells were seeded in a 96-well plate at a density of 5 × 103 cells/well in DMEM medium, supplemented with 10% FBS, and incubated

Manipulation of isolated brain nerve terminals by an external magnetic field using D-mannose-coated γ-Fe₂O₃ nano-sized particles and assessment of their effects on glutamate transport

• Tatiana Borisova,
• Natalia Krisanova,
• Arsenii Borуsov,
• Roman Sivko,
• Ludmila Ostapchenko,
• Michal Babic and
• Daniel Horak

Beilstein J. Nanotechnol. 2014, 5, 778–788, doi:10.3762/bjnano.5.90

• cells possess receptors for D-mannose on their membranes, that is, MMR on dendritic cells subsets, macrophages, lymphatic and hepatic endothelium, Endo 180 on subsets of endothelial cells, DC-SIGNR on hepatic and lymphatic endothelial cells as well as serum contains mannose binding lectines (MBL) [10

• nanoparticles to penetrate the blood–brain barrier increased significantly in the presence of an external magnetic force. Therefore, particles can be transported through the blood–brain barrier and taken up by astrocytes, while they do not affect the viability of the endothelial cells [26]. On the cellular