Influence of the PDMS substrate stiffness on the adhesion of Acanthamoeba castellanii

• Sören B. Gutekunst,
• Carsten Grabosch,
• Alexander Kovalev,
• Stanislav N. Gorb and
• Christine Selhuber-Unkel

Beilstein J. Nanotechnol. 2014, 5, 1393–1398, doi:10.3762/bjnano.5.152

• parameters such as cell proliferation, as the doubling time of acanthamoeba in axenic culture is on the timescale of days [38]. As acanthamoeba can also be grown in suspension [19], their proliferation might not be strongly influenced by the presence of any substrate. In a recent study, we had investigated

Mimicking exposures to acute and lifetime concentrations of inhaled silver nanoparticles by two different in vitro approaches

• Fabian Herzog,
• Kateryna Loza,
• Sandor Balog,
• Martin J. D. Clift,
• Matthias Epple,
• Peter Gehr,
• Alke Petri-Fink and
• Barbara Rothen-Rutishauser

Beilstein J. Nanotechnol. 2014, 5, 1357–1370, doi:10.3762/bjnano.5.149

• . Others found dissolution and thus a subsequent release of Ag ions from PVP-coated Ag NPs that were synthesized similar to the particles used in the current study [64]. Other studies using the same PVP-capped Ag NPs as we used, showed a similar aggregation pattern as we found [46]. Furthermore, cell

• proliferation and migration (chemotaxis) both decreased, and the release of cytokines was affected. Increased IL-8 and decreased IL-6 and vascular endothelial growth factor (VEGF) levels were detected at high Ag NP concentrations [65]. These studies however, were obtained with human mesenchymal stem cells

PEGylated versus non-PEGylated magnetic nanoparticles as camptothecin delivery system

• Paula M. Castillo,
• Mario de la Mata,
• Maria F. Casula,
• José A. Sánchez-Alcázar and
• Ana P. Zaderenko

Beilstein J. Nanotechnol. 2014, 5, 1312–1319, doi:10.3762/bjnano.5.144

• ; cancer therapy; iron oxide superparamagnetic nanoparticles; polyethylene glycol; Introduction Camptothecin (CPT) is a quinoline based alkaloid, which exhibits a potent cytotoxic activity against a broad spectrum of tumours [1][2][3]. While most antineoplastic agents inhibit cancer cell proliferation by

Antimicrobial nanospheres thin coatings prepared by advanced pulsed laser technique

• Alina Maria Holban,
• Valentina Grumezescu,
• Alexandru Mihai Grumezescu,
• Bogdan Ştefan Vasile,
• Roxana Truşcă,
• Rodica Cristescu,
• Gabriel Socol and
• Florin Iordache

Beilstein J. Nanotechnol. 2014, 5, 872–880, doi:10.3762/bjnano.5.99

• (Sigma Aldrich, St. Louis, MO, USA). For cell proliferation and viability CellTiter96 Non-Radioactive Cell Proliferation Assay, (Promega, Madison, USA) was used. Endothelial cells were seeded in a 96-well plate at a density of 5 × 103 cells/well in DMEM medium, supplemented with 10% FBS, and incubated

• with nanospheres coated with eugenol for 72 h. The controls were represented by endothelial cells grown under the same culture conditions, but on bare substrates. Following the guidelines of the manufacturer the cell proliferation assay was performed in triplicates at different time intervals. Briefly

Nanoglasses: a new kind of noncrystalline materials

• Herbert Gleiter

Beilstein J. Nanotechnol. 2013, 4, 517–533, doi:10.3762/bjnano.4.61

• microstructure of nanoglasses on the bioactivity, hierarchically structured layers of Ti34Zr14Cu22Pd30 metallic nanoglass were created by magnetron sputtering. The cell proliferation on the surfaces of these materials was studied by seeding ten thousand osteoblasts on the free surface of the Ti34Zr14Cu22Pd30

• higher than that on the surface of the corresponding melt-spun ribbon. Moreover, it was about five-fold and about ten-times higher than the cell densities on surfaces of the MGR and MGS ribbons, respectively. This high level of cell proliferation does not seem to be caused primarily by the surface

• 16% total strain. Right: Atomic shear strain in a Cu36Zr64 nanoglass of 10 nm grain diameter at 8% and 16% total strain. The symbols show the position of Cu- and Zr-rich grains, respectively. Reproduced with permission from [52]. Cell proliferation at the surface of a melt-spun ribbon and at the

Magnetic-Fe/Fe₃O₄-nanoparticle-bound SN38 as carboxylesterase-cleavable prodrug for the delivery to tumors within monocytes/macrophages

• Hongwang Wang,
• Tej B. Shrestha,
• Matthew T. Basel,
• Raj K. Dani,
• Gwi-Moon Seo,
• Sivasai Balivada,
• Marla M. Pyle,
• Heidy Prock,
• Olga B. Koper,
• Prem S. Thapa,
• David Moore,
• Ping Li,
• Viktor Chikan,
• Deryl L. Troyer and
• Stefan H. Bossmann

Beilstein J. Nanotechnol. 2012, 3, 444–455, doi:10.3762/bjnano.3.51

• . Different concentrations of nanoparticles were taken up by double-stable Mo/Ma cells over 24 h; the nanoparticle-concentration ranged from 0 to 320 μg/mL MNP-SN38 in fresh medium. After 24 h, the inhibition of cell proliferation was measured by using the MTT assay (Figure 4). We found only 20% of inhibition

• of cell proliferation at 160 μg/mL. Our aim is the loading of high payloads onto each delivery cell without causing a high level of necrosis or apoptosis of the delivery cells. Even a loading of 320 μg/mL of nanoparticles in the medium inhibits only 50% of the cell proliferation. We are also

• of nanoparticles contained 0.427 mg of iron, indicating that this amount of iron would be high enough for alternating magnetic field hyperthermia in combination with chemotherapy [54]. The MTT assay indicated that 8 pg of iron can be easily loaded in each cell (20% inhibition of cell proliferation