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Search for "cellular uptake" in Full Text gives 129 result(s) in Beilstein Journal of Nanotechnology.
Targeting strategies for improving the efficacy of nanomedicine in oncology
Beilstein J. Nanotechnol. 2019, 10, 168–181, doi:10.3762/bjnano.10.16
- cellular targeting moieties on the nanocarrier surface. Through tissular targeting the nanocarrier would be directed to the diseased cells improving its accumulation. Once there, the presence of the cellular targeting moieties would enhance the cellular uptake into the tumoral cells. In several types of
Surface plasmon resonance enhancement of photoluminescence intensity and bioimaging application of gold nanorod@CdSe/ZnS quantum dots
Beilstein J. Nanotechnol. 2019, 10, 22–31, doi:10.3762/bjnano.10.3
- @CdSe/ZnS can be used in cell imaging studies, after conjugation with FA, we performed a MCF-7 breast cancer cell targeting imaging study, and the results as shown in Figure 8. Robust cellular uptake could be obtained from the CdSe/ZnS@FA and GNR@CdSe/ZnS@FA treated samples. The obvious luminescence and
The nanoscaled metal-organic framework ICR-2 as a carrier of porphyrins for photodynamic therapy
Beilstein J. Nanotechnol. 2018, 9, 2960–2967, doi:10.3762/bjnano.9.275
- substituent at the porphyrin phosphinate groups. Thus, phosphinatophenylporphyrin with phenyl substituents has the strongest photodynamic efficacy due to the most efficient cellular uptake. Keywords: metal-organic framework; phosphinic acid based MOF; photodynamic therapy; porphyrin; singlet oxygen
- the proximity of iron atoms constituting the ICR-2 structure. It is worth noting that the porphyrin loading does not affect the fluorescence quantum yields. Photobiological properties Cellular uptake and intracellular localization HeLa cells were treated with the nanoparticles in Eagle's Minimum
- Essential Medium (EMEM) without foetal bovine serum to avoid modification of the particle surface properties by nonspecific binding of serum albumin. The cellular uptake of the nanoparticles was quantified by flow-cytometry analysis of porphyrin fluorescence associated with the cells. Figure 6A shows the
Cytotoxicity of doxorubicin-conjugated poly[N-(2-hydroxypropyl)methacrylamide]-modified γ-Fe2O3 nanoparticles towards human tumor cells
Beilstein J. Nanotechnol. 2018, 9, 2533–2545, doi:10.3762/bjnano.9.236
- undifferentiated cells, mesenchymal stem cells were utilized. Cellular uptake of agents was studied by fluorescence microscopy and induction of cell death was visualized by live/dead assay. Dox-conjugated γ-Fe2O3@P(HPMA-MMAA) particles showed enhanced cytotoxicity in drug-sensitive and drug-resistant tumor cells
- attached to the cells. The cellular uptake was measured by fluorescence microscopy with a Keyence Biorevo BZ-9000 instrument (Osaka, Japan) equipped with filters for Texas Red (EX 560/40, DM 585, BA 630/75) at 20× magnification. Dox and its complexes were visible as red fluorescing dots. Live/dead assay
- their enhanced accumulation inside the cells. The particles are able to penetrate cells by the selected type of endocytosis mechanism: phagocytosis, pinocytosis, or receptor mediated endocytosis [25]. In order to check this hypothesis, cellular uptake of γ-Fe2O3@P(HPMA-MMAA)-Dox nanoparticles was
Enhanced antineoplastic/therapeutic efficacy using 5-fluorouracil-loaded calcium phosphate nanoparticles
Beilstein J. Nanotechnol. 2018, 9, 2499–2515, doi:10.3762/bjnano.9.233
- with 5-FU and resulted in enhanced cellular uptake and efficacy [18]. Furthermore, a chitosan/gold nanocomposite was employed as a load carrier for 5-FU with an encapsulation efficiency of 96% [19]. Interestingly, monomeric self-assembled nucleoside nanoparticles (SNNPs) loaded with 5-FU were shown to
- agglomeration and enhanced colloidal stability [29]. Since the particle stability mainly depends on the electrical charge of the surface, properties such as cellular uptake, rate of drug release, and blood retention time are directly correlated with the zeta potential value. Furthermore, the zeta potential
- showed a concentration-dependent toxicity on the cells irrespective of the cell lines used. In NIH 3T3 cells, considerably low toxicity was found (Figure 4A) with respect to cancer cells. The increased metabolism in cancer cells might be the reason for the enhanced cellular uptake [39] of the particles
Review on nanoparticles and nanostructured materials: history, sources, toxicity and regulations
Beilstein J. Nanotechnol. 2018, 9, 1050–1074, doi:10.3762/bjnano.9.98
Green synthesis of fluorescent carbon dots from spices for in vitro imaging and tumour cell growth inhibition
Beilstein J. Nanotechnol. 2018, 9, 530–544, doi:10.3762/bjnano.9.51
- observed between the absolute value of the zeta potential and the cellular uptake observed in both LN-229 and HK-2 cells. XRD patterns of the four synthesized C-dots have been also studied, and the obtained results are given in Figure 5. The cinnamon, red chilli, turmeric and black pepper C-dots
Involvement of two uptake mechanisms of gold and iron oxide nanoparticles in a co-exposure scenario using mouse macrophages
Beilstein J. Nanotechnol. 2017, 8, 2396–2409, doi:10.3762/bjnano.8.239
- treated with endocytotic inhibitors recovered after co-exposure, which additionally hinted that two uptake mechanisms are involved. Cross-talk between uptake pathways is relevant for toxicological studies: Co-exposure acts as an uptake accelerant. If the goal is to maximize the cellular uptake, e.g., for
- the effects of exposure to each individual NP [26]. Moreover, little is known about how co-exposure of different NPs affects cellular uptake (e.g., whether it enhances or even inhibits selective NP uptake), which is –particularly in nanomedicine– highly relevant to the understanding of their behaviour
- in a complex colloidal system, such as the vascular system. The aim of this work was to study the combined effect of two model NPs on cellular uptake, that is gold (AuNPs) and iron oxide NPs (FeOxNPs) stabilized with the same polymer shell and incorporated fluorophores. The NPs can be distinguished
Carbon nano-onions as fluorescent on/off modulated nanoprobes for diagnostics
Beilstein J. Nanotechnol. 2017, 8, 1878–1888, doi:10.3762/bjnano.8.188
- development of a nanosensor which can be “turned on” in an acidic environment. Remarkably, the fluo-CNOs maintained the switching properties upon cell internalization, as they were “switched-on” in response to acidic pH. In vitro experiments on HeLa cells showed excellent cellular uptake and low toxicity of
- fluo-CNOs were successfully internalized by cells and were distributed throughout the cytoplasm. Remarkably the cellular uptake of the fluo-CNOs was clearly observed soon after the incubation, as shown by the presence of CNOs inside the cell after 2 h (Figure 8A). After 12 and 48 h of incubation
- 1 h, pH 4.5; Scale bars = 20 μm. Cellular uptake and localization of fluo-CNOs in HeLa cells in acidic conditions (PBS, pH 4.5) observed by confocal fluorescence microscopy after incubation for 2 h (A), 12 h (B) and 48 h (C), respectively. Scale bars = 20 μm. Three-dimensional reconstruction by
Development of polycationic amphiphilic cyclodextrin nanoparticles for anticancer drug delivery
Beilstein J. Nanotechnol. 2017, 8, 1457–1468, doi:10.3762/bjnano.8.145
- particle size of nanoparticulate drug delivery systems play a direct and important role on cellular uptake, systemic circulation, toxicity and stability of nanoparticles [37][38]. It was reported that nanoparticles smaller than 200 nm can escape recognition by the mononuclear phagocytic system (MPS) [39
- negatively charged molecules (e.g., sialic acid, cholesterol, phospholipid) on cell membrane easier than anionic nanoparticles [26][52]. In addition, the surface charge of nanoparticles play an important role on cellular uptake and subcellular localization [53][54]. Another reason for the cell viability
Cationic PEGylated polycaprolactone nanoparticles carrying post-operation docetaxel for glioma treatment
Beilstein J. Nanotechnol. 2017, 8, 1446–1456, doi:10.3762/bjnano.8.144
- agents to the tumor site, while avoiding possible side effects. The development of novel drug delivery systems with reduced side effects is an important breakthrough and nanoparticles are promising in this field as they enable localized drug delivery to target sites and enhanced cellular uptake
- –shell PCL nanoparticles to tumor targeting with docetaxel on a glioma model is very rare. Recently, active-targeted docetaxel-loaded PEG/PCL nanoparticles were prepared successfully for glioblastoma therapy by Gao et al. Cellular uptake and tumor spheroid uptake studies on U87 human glioma cells show
- obtained by dual drug targeting. Besides that, the magnetic and ligand targeting resulted in elevated cellular uptake of nanocapsules in glioma treatment [26]. Yang et al. successfully obtained targeted and traceable core–shell nanoparticles for carmustine (BCNU) delivery. These systems prolonged the half
Carbon nanomaterials sensitize prostate cancer cells to docetaxel and mitomycin C via induction of apoptosis and inhibition of proliferation
Beilstein J. Nanotechnol. 2017, 8, 1307–1317, doi:10.3762/bjnano.8.132
- S1c,d) [28]. Consistently, the reduction of the chemotherapeutic dosage was more distinct in combinatory treatments with CNFs independent of the chemotherapeutic used. A combined application with carbon nanomaterials would also result in an improved cellular uptake of the active drug component as
Nano-engineered skin mesenchymal stem cells: potential vehicles for tumour-targeted quantum-dot delivery
Beilstein J. Nanotechnol. 2017, 8, 1218–1230, doi:10.3762/bjnano.8.123
- optimal conditions for cellular uptake experiments [39]. Given the lack of standardized NP uptake conditions in MSCs, we adjusted the protocol for QD uptake in human skin MSCs. The results showed that a 6 h incubation with 8 or 16 nM QDs is optimal for QD accumulation in more than 95% of the MSC
- QD uptake in skin MSCs. The composition of the protein corona could either enhance or decrease the cellular uptake of polystyrene-based NPs, depending on nanoparticle functionalization [47]. We have showed that NP uptake in skin MSCs is an active process and does not occur passively. For the
Dispersion of single-wall carbon nanotubes with supramolecular Congo red – properties of the complexes and mechanism of the interaction
Beilstein J. Nanotechnol. 2017, 8, 636–648, doi:10.3762/bjnano.8.68
- length influences CNT toxicity and cellular uptake [22][23]. Surfactants commonly used for dispersion of CNTs include SDS, CTAB, Triton X-100 or sodium cholate [24][25][26]. A less known approach is based on the interaction of CNTs with a bis-azo dye – Congo red (CR) [27]. This original procedure was
Uptake of the proteins HTRA1 and HTRA2 by cells mediated by calcium phosphate nanoparticles
Beilstein J. Nanotechnol. 2017, 8, 381–393, doi:10.3762/bjnano.8.40
- electron microscopy. Various cell types (HeLa, MG-63, THP-1, and hMSC) were incubated with fluorescently labelled proteins alone or with protein-loaded cationic and anionic nanoparticles. The cellular uptake was followed by light and fluorescence microscopy, confocal laser scanning microscopy (CLSM), and
- cellular uptake, calcium phosphate nanoparticles are dissolved in the acidified lysosomes and finally excreted in ionic form [28]. The high temperature requirement A (HtrA) family of serine proteases belongs to the core set of proteases found in cells and is widely conserved in single and multicellular
- nm, emission: 460 nm) channels. Confocal laser scanning microscopy (CLSM) was performed on a Leica SP5 confocal inverse CLSM. The cells were stained with DAPI (nucleus) and Cell mask™ (cell membrane) to indicate the cellular uptake of the nanoparticles. To elucidate the uptake mechanism, several
Facile fabrication of luminescent organic dots by thermolysis of citric acid in urea melt, and their use for cell staining and polyelectrolyte microcapsule labelling
Beilstein J. Nanotechnol. 2016, 7, 1905–1917, doi:10.3762/bjnano.7.182
- . Monitoring of cellular uptake of LbL-microcapsules decorated with O-dots Murine macrophages readily take up microcapsules decorated with O-dots. By using different sets of filters, it is possible to register the multicoloured fluorescence of phagocytized microcapsules in vitro (Figure 7). Thus, this property
- , Russia). Cultures were incubated at 37 °C in air containing 5% CO2. Cells growing exponentially were harvested by a brief incubation with 0.25% trypsin–ethylenediaminetetraacetic acid (EDTA) solution (Gibco). The cellular uptake of microcapsules was studied using RAW 264.7 murine macrophage-like cell
On the pathway of cellular uptake: new insight into the interaction between the cell membrane and very small nanoparticles
Beilstein J. Nanotechnol. 2016, 7, 1296–1311, doi:10.3762/bjnano.7.121
Straightforward and robust synthesis of monodisperse surface-functionalized gold nanoclusters
Beilstein J. Nanotechnol. 2016, 7, 1278–1283, doi:10.3762/bjnano.7.118
- synthesis without dialysis. Glc-NCs were not cytotoxic at any concentration tested (Figure 3B), whereas THPC-NCs were toxic at 100 µM (Figure S14, Supporting Information File 1), indicating that Glc-NCs are suitable for biological experiments even without purification. Cellular uptake of the nanoclusters
- lectin ConA (4). Cell viability of Glc-NCs A) purified and B) without purification incubated for one day with L929 cells. The Glc-NCs were not toxic at any of the concentrations studied. C) Cellular uptake of Glc-NCs when incubated with L929 cells for one day. Gold concentration taken up by the cells was
Improved biocompatibility and efficient labeling of neural stem cells with poly(L-lysine)-coated maghemite nanoparticles
Beilstein J. Nanotechnol. 2016, 7, 926–936, doi:10.3762/bjnano.7.84
- , 16206 Prague 6, Czech Republic 10.3762/bjnano.7.84 Abstract Background: Cell tracking is a powerful tool to understand cellular migration, dynamics, homing and function of stem cell transplants. Nanoparticles represent possible stem cell tracers, but they differ in cellular uptake and side effects
- their cellular uptake, the mechanism of internalization, cytotoxicity, viability and proliferation of neural stem cells, and compared them to the commercially available dextran-coated nanomag®-D-spio nanoparticles. Results: Light microscopy of Prussian blue staining revealed a concentration-dependent
- of neural stem cells. Cytochalasine D blocked the cellular uptake of nanoparticles indicating an actin-dependent process, such as macropinocytosis, to be the internalization mechanism for both nanoparticle types. Finally, immunocytochemistry analysis of neural stem cells after treatment with poly(L
Tight junction between endothelial cells: the interaction between nanoparticles and blood vessels
Beilstein J. Nanotechnol. 2016, 7, 675–684, doi:10.3762/bjnano.7.60
- stress found that without shear stress, the cellular uptake/association of both PDA-coated liposomes (LPDA) and LPDA-PEG for hepatocytes were quite similar, while myoblasts preferred to internalize/associate with LPDA. However, under shear stress, hepatocytes showed its preference to LPDA after 30 min
Comparison of the interactions of daunorubicin in a free form and attached to single-walled carbon nanotubes with model lipid membranes
Beilstein J. Nanotechnol. 2016, 7, 524–532, doi:10.3762/bjnano.7.46
- mechanisms proposed to explain the cellular uptake of CNTs including the passive diffusion in a non-invasive manner (tiny nanoneedle mechanism) [18]. Carbon nanotubes have been successfully used to transport different types of anticancer agents including camptothecin, doxorubicin and daunorubicin [19]. The
Surface coating affects behavior of metallic nanoparticles in a biological environment
Beilstein J. Nanotechnol. 2016, 7, 246–262, doi:10.3762/bjnano.7.23
- media like dissolution, adsorption, binding, and aggregation, all influencing biological impacts by affecting reactive oxygen species generation, cellular uptake and NP biodistribution [15][16][17][18]. Metallic NPs usually aggregate in media with high electrolyte content that correspond to biological
Atomic force microscopy as analytical tool to study physico-mechanical properties of intestinal cells
Beilstein J. Nanotechnol. 2015, 6, 1457–1466, doi:10.3762/bjnano.6.151
- , differing in lengths as well as diameters. This suggests that the absence of a well-developed brush border in M cells may facilitate the adherence of antigens on the cell surface and, as a consequence, cellular uptake processes [2]. By contrast, the large surface area of intestinal microvilli is more
- fill remaining data gaps on the effects of these parameters on cell mechanics/kinetics and, as a consequence, on cellular uptake processes (e.g., nanoparticulate systems/antigens). Conclusion The current study shows that cytoskeletal structures and the content of F-actin filaments strongly impact
- properties of cells can be directly linked to cell adhesion, adhesion to the smooth and more elastic surface of M cells is enhanced, thus, facilitating the adherence of antigens and, as a consequence, cellular uptake processes. Experimental Cell cultures Raji B cells were a kind gift from R. Fuchs (Medical
PLGA nanoparticles as a platform for vitamin D-based cancer therapy
Beilstein J. Nanotechnol. 2015, 6, 1306–1318, doi:10.3762/bjnano.6.135
- calcitriol remained stable at release conditions throughout the experiment period. Cellular uptake of PLGA NPs and calcitriol-induced morphological changes The internalization of fluorescent C6–calcitriol–PLGA NPs by S2-013, hTERT-HPNE and A549 cells was evaluated by confocal microscopy. Counterstaining of
Protein corona – from molecular adsorption to physiological complexity
Beilstein J. Nanotechnol. 2015, 6, 857–873, doi:10.3762/bjnano.6.88
- composition and the latest findings that help to shed light on temporal evolution of the full serum corona for the first time. Finally, we discuss the most recent advances regarding the molecular-scale mechanistic role of the protein corona in cellular uptake of NPs. Keywords: agglomeration; corona
- charge [4][9] are critical factors determining the formation and nature of the protein corona. The composition and molecular properties of the protein corona have been shown to be influential factors for the cellular uptake of NPs [7][10][11][12] but direct links between structure and effect remain to be
- biological environment. The formation of the biomolecular corona around the NPs modulated their behavior: cells of the blood system seemed to be protected against NP-induced (patho)biological processes by the presence of the corona and also cellular uptake could be promoted [10]. Effect of the corona
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