Functional fusion of living systems with synthetic electrode interfaces

• Oskar Staufer,
• Sebastian Weber,
• C. Peter Bengtson,
• Hilmar Bading,
• Joachim P. Spatz and
• Amin Rustom

Beilstein J. Nanotechnol. 2016, 7, 296–301, doi:10.3762/bjnano.7.27

• diagnostics utilizing biomolecular machineries “perfected” during billion years of evolution. To date, hardware–wetware interfaces that sample or modulate bioelectric potentials, such as neuroprostheses or implantable energy harvesters, are mostly based on microelectrodes brought into the closest possible

Mismatch detection in DNA monolayers by atomic force microscopy and electrochemical impedance spectroscopy

• Maryse D. Nkoua Ngavouka,
• Pietro Capaldo,
• Elena Ambrosetti,
• Giacinto Scoles,
• Loredana Casalis and
• Pietro Parisse

Beilstein J. Nanotechnol. 2016, 7, 220–227, doi:10.3762/bjnano.7.20

• diagnostics of SNPs. Moreover a comparative analysis between the two techniques allows for a deep understanding of hybridization processes in the presence of single and multiple mismatches. Experimental Fabrication and measurement processes of AFM-based assays Gold-coated substrates were immersed in 300 μM of

• . Due to the relatively simple geometry, the device could be easily further miniaturized and integrated in multiplexed arrays through microfluidic systems, allowing for point-of-care diagnostics. Our results demonstrated that nano-mechanical and EIS strategies are state of the art for the detection of

Single-molecule mechanics of protein-labelled DNA handles

• Vivek S. Jadhav,
• Dorothea Brüggemann,
• Florian Wruck and
• Martin Hegner

Beilstein J. Nanotechnol. 2016, 7, 138–148, doi:10.3762/bjnano.7.16

• -digoxigenin antibodies (200 µg/mL in PBS; Roche Diagnostics, Rotkreuz, Switzerland) were incubated for 1 h at room temperature and washed once by pelleting in 0.05 M sodium tetraborate, pH 8.2. The anti-DIG antibodies were then cross-linked to the protein G beads using 10 mg/mL DMP crosslinker (Pierce/Fisher

Application of biclustering of gene expression data and gene set enrichment analysis methods to identify potentially disease causing nanomaterials

• Andrew Williams and
• Sabina Halappanavar

Beilstein J. Nanotechnol. 2015, 6, 2438–2448, doi:10.3762/bjnano.6.252

• applications [43]. Current applications of NMs include therapeutic applications (e.g., nanomedicine, drug delivery, diagnostics), agriculture, manufacturing, electronics, cosmetics, textiles, and environmental remediation and protection. Although NMs are synthesized from their corresponding, known, bulk

Optimized design of a nanostructured SPCE-based multipurpose biosensing platform formed by ferrocene-tethered electrochemically-deposited cauliflower-shaped gold nanoparticles

• Wicem Argoubi,
• Maroua Saadaoui,
• Sami Ben Aoun and
• Noureddine Raouafi

Beilstein J. Nanotechnol. 2015, 6, 1840–1852, doi:10.3762/bjnano.6.187

• ]. Rapid diagnostics in war zones, remote areas or on-field monitoring of warfare agents, explosives, pesticides and herbicides are few among many applications of such a technology. The elaboration of sensors to satisfy these needs is highly regarded [4][5]. Screen-printed electrodes on polymeric

Experiences in supporting the structured collection of cancer nanotechnology data using caNanoLab

• Stephanie A. Morris,
• Sharon Gaheen,
• Michal Lijowski,
• Mervi Heiskanen and
• Juli Klemm

Beilstein J. Nanotechnol. 2015, 6, 1580–1593, doi:10.3762/bjnano.6.161

• review, comparison, and re-use, with the ultimate goal of accelerating the translation of nanotechnology-based cancer therapeutics, diagnostics, and imaging agents to the clinic. In this paper, we will discuss challenges associated with developing a nanomaterial database and recognized needs for

• burden of cancer is clear, calling for earlier detection and treatment modalities to alleviate this problem. Standard cancer therapeutics are often characterized by poor water solubility and rapid degradation leading to narrow therapeutic windows and doses limited by toxicity [3]. In turn, diagnostics

• are often hindered at the level of sensitivity, and time between testing and diagnosis. Opportunities for the potential to improve current cancer therapeutics and diagnostics are sorely needed. Nanotechnology provides tremendous opportunities in applications to medicine to make improvements in both

DNA–melamine hybrid molecules: from self-assembly to nanostructures

• Rina Kumari,
• Shib Shankar Banerjee,
• Anil K. Bhowmick and
• Prolay Das

Beilstein J. Nanotechnol. 2015, 6, 1432–1438, doi:10.3762/bjnano.6.148

• molecules can result in unique DNA-based nanostructures for application in molecular and cellular biophysics, as biomimetic systems, in energy transfer and photonics, and in diagnostics and therapeutics [18][19][20][21]. Moreover, as a bottom-up technique, such a methodology can contribute to molecular

• , and others [41][42][43][44]. The large increase in stability and melting temperature of the short duplexes could find a number of applications in biotechnology, such as in more sensitive DNA detection and diagnostics [30]. The self-assembled DNA–organic molecule hybrid forms a linear nanoassembly that

Pulmonary surfactant augments cytotoxicity of silica nanoparticles: Studies on an in vitro air–blood barrier model

• Jennifer Y. Kasper,
• Lisa Feiden,
• Maria I. Hermanns,
• Christoph Bantz,
• Michael Maskos,
• Ronald E. Unger and
• C. James Kirkpatrick

Beilstein J. Nanotechnol. 2015, 6, 517–528, doi:10.3762/bjnano.6.54

• day at a dilution of 1:3 and 1:6 until passage 50 and 35, respectively. Monocultures (MC) in experimental procedures: Prior to seeding cells, the 96-well plates (TPP, Switzerland) or 8 well µ-slides (ibidi) were coated with 50/300 µL fibronectin for 1 h at 37 °C (5 µg/mL, Roche Diagnostics, Mannheim

Synergic combination of the sol–gel method with dip coating for plasmonic devices

• Cristiana Figus,
• Maddalena Patrini,
• Francesco Floris,
• Lucia Fornasari,
• Paola Pellacani,
• Gerardo Marchesini,
• Andrea Valsesia,
• Flavia Artizzu,
• Daniela Marongiu,
• Michele Saba,
• Franco Marabelli,
• Andrea Mura,
• Giovanni Bongiovanni and
• Francesco Quochi

Beilstein J. Nanotechnol. 2015, 6, 500–507, doi:10.3762/bjnano.6.52

• conical poly(methyl methacrylate) (PMMA) pillars [32][33][34]. Such a plasmonic platform has been shown to work efficiently as an SPR-sensitive surface for biosensing applications in medical diagnostics [35]. A sketch of the pillar profile along a normal cross section is provided in Figure 4, where the

Tunable light filtering by a Bragg mirror/heavily doped semiconducting nanocrystal composite

• Ilka Kriegel and
• Francesco Scotognella

Beilstein J. Nanotechnol. 2015, 6, 193–200, doi:10.3762/bjnano.6.18

• very interesting for point-of-care diagnostics [1][2]. Very interesting strategies to fabricate colour filters, without auto-fluorescence, are proposed in the literature [3]. However, in many applications, such as in microfluidic devices, where the detection of more than one analyte is often necessary

Synthesis of boron nitride nanotubes and their applications

• Saban Kalay,
• Zehra Yilmaz,
• Ozlem Sen,
• Melis Emanet,
• Emine Kazanc and
• Mustafa Çulha

Beilstein J. Nanotechnol. 2015, 6, 84–102, doi:10.3762/bjnano.6.9

• combined with the properties of other nanomaterials to construct novel sensor devices for humidity, carbon dioxide detection, and clinical diagnostics. A highly sensitive humidity sensor using BNNTs and silver nanoparticles (AgNPs) for the rapid detection of humidity was fabricated [90]. Figure 8 shows the

• suggested that the Ni-encapsulated BNNTs could be used in clinical diagnostics and bioimaging applications due to their TRPL properties [91]. Hydrogen storage in BNNTs Hydrogen is considered to be an exceptional energy source material since it produces clean energy in high yield. Although there are several

Proinflammatory and cytotoxic response to nanoparticles in precision-cut lung slices

• Stephanie Hirn,
• Nadine Haberl,
• Kateryna Loza,
• Matthias Epple,
• Wolfgang G. Kreyling,
• Barbara Rothen-Rutishauser,
• Markus Rehberg and
• Fritz Krombach

Beilstein J. Nanotechnol. 2014, 5, 2440–2449, doi:10.3762/bjnano.5.253

• ], viability was determined as ratio of nuclei of dead cells per volume of live cells (spots (diameter ≤ 4 µm)/volume (105 µm3). WST-1 reduction in PCLS culture medium The WST-1 assay (Roche Diagnostics, Mannheim, Germany) was used for spectrometric quantification of cellular viability. After removing cell

Interaction of dermatologically relevant nanoparticles with skin cells and skin

• Annika Vogt,
• Fiorenza Rancan,
• Sebastian Ahlberg,
• Berouz Nazemi,
• Chun Sik Choe,
• Maxim E. Darvin,
• Sabrina Hadam,
• Ulrike Blume-Peytavi,
• Kateryna Loza,
• Jörg Diendorf,
• Matthias Epple,
• Christina Graf,
• Eckart Rühl,
• Martina C. Meinke and
• Jürgen Lademann

Beilstein J. Nanotechnol. 2014, 5, 2363–2373, doi:10.3762/bjnano.5.245

• microscopic measurements were performed by using the skin composition analyzer (River Diagnostics, Model 3510, Rotterdam, The Netherlands). The fingerprint region (400–2000 cm−1) excited by near-infrared laser radiation (785 nm, 25 mW on the skin) was used for sample analyses. Raman spectra were recorded from

Inorganic Janus particles for biomedical applications

• Isabel Schick,
• Steffen Lorenz,
• Dominik Gehrig,
• Stefan Tenzer,
• Wiebke Storck,
• Karl Fischer,
• Dennis Strand,
• Frédéric Laquai and
• Wolfgang Tremel

Beilstein J. Nanotechnol. 2014, 5, 2346–2362, doi:10.3762/bjnano.5.244

• precursors were mixed simultaneously. Janus particles as multimodal contrast agents Recent developments in the field of nanoparticles for biomedical applications have increased the interest in multifunctional nanoparticles for theranostics, a combination of therapy and diagnostics, which was realized with

• resonance. Additionally, the gold nanoparticles exhibit a strong X-ray absorption, which can be used to increase the contrast in CT diagnostics [90], as shown by the combination of gold nanoparticles with iron oxide to create multifunctional hetero-nanoparticles for simultaneous MRI and CT imaging [66][91

Coating with luminal gut-constituents alters adherence of nanoparticles to intestinal epithelial cells

• Heike Sinnecker,
• Katrin Ramaker and
• Andreas Frey

Beilstein J. Nanotechnol. 2014, 5, 2308–2315, doi:10.3762/bjnano.5.239

• Heike Sinnecker Katrin Ramaker Andreas Frey Division of Mucosal Immunology & Diagnostics, Priority Program Asthma & Allergy, Research Center Borstel, Leibniz Center for Medicine and Biosciences, Parkallee 22, 23845 Borstel, Germany, Member of the German Center for Lung Research 10.3762/bjnano

Nanoencapsulation of ultra-small superparamagnetic particles of iron oxide into human serum albumin nanoparticles

• Matthias G. Wacker,
• Mahmut Altinok,
• Stephan Urfels and
• Johann Bauer

Beilstein J. Nanotechnol. 2014, 5, 2259–2266, doi:10.3762/bjnano.5.235

• with the surface modification and nanoencapsulation of USPIO into an albumin matrix by using ethanolic desolvation. Particles of narrow size distribution and with a defined particle structure have been achieved. Keywords: diagnostics; HSA; nanoencapsulation; nanoparticles; USPIO; Introduction Over

The gut wall provides an effective barrier against nanoparticle uptake

• Heike Sinnecker,
• Thorsten Krause,
• Sabine Koelling,
• Ingmar Lautenschläger and
• Andreas Frey

Beilstein J. Nanotechnol. 2014, 5, 2092–2101, doi:10.3762/bjnano.5.218

• Heike Sinnecker Thorsten Krause Sabine Koelling Ingmar Lautenschlager Andreas Frey Division of Mucosal Immunology & Diagnostics, Priority Program Asthma & Allergy, Research Center Borstel, Leibniz Center for Medicine and Biosciences, Parkallee 22, Borstel, 23845, Germany, Airway Research Center

Influence of surface-modified maghemite nanoparticles on in vitro survival of human stem cells

• Michal Babič,
• Daniel Horák,
• Lyubov L. Lukash,
• Tetiana A. Ruban,
• Yurii N. Kolomiets,
• Svitlana P. Shpylova and
• Oksana A. Grypych

Beilstein J. Nanotechnol. 2014, 5, 1732–1737, doi:10.3762/bjnano.5.183

• labeling of cells in order to track them both in diagnostics and therapeutics [1][2]. For example, mesenchymal [3], neural [4], and bone marrow [5] stem cells, as well as other cells are widely labeled by surface-coated iron oxide nanoparticles. Other applications of nanoparticles involve the delivery of

• cells. Surface modifications are already well described as the particles are used in many applications, such as magnetic contrast agents, separations, diagnostics, drug delivery, and hyperthermia [13][14][15][16][17]. In terms of coating, many low- and high-molecular-weight compounds were proposed, e.g

Donor–acceptor graphene-based hybrid materials facilitating photo-induced electron-transfer reactions

• Anastasios Stergiou,
• Georgia Pagona and
• Nikos Tagmatarchis

Beilstein J. Nanotechnol. 2014, 5, 1580–1589, doi:10.3762/bjnano.5.170

• , although graphene-based biomaterials are out of the scope of this mini-review, GO has been covalently functionalized with peptides, antibodies and other biomolecules for applications in diagnostics, novel therapeutic approaches and near infrared (NIR) photo-thermal therapies [53]. A representative work

Protein-coated pH-responsive gold nanoparticles: Microwave-assisted synthesis and surface charge-dependent anticancer activity

• Dickson Joseph,
• Nisha Tyagi,
• Christian Geckeler and
• Kurt E.Geckeler

Beilstein J. Nanotechnol. 2014, 5, 1452–1462, doi:10.3762/bjnano.5.158

• cell viability for cancerous cells in comparison to fibroblasts. Hence, increasing evidence from these studies is pointing that by controlling the concentrations of the AuNPs, anticancer agents can be formulated. These synthesized AuNPs show promise for use in biomedical applications for diagnostics

Cytotoxic and proinflammatory effects of PVP-coated silver nanoparticles after intratracheal instillation in rats

• Nadine Haberl,
• Stephanie Hirn,
• Alexander Wenk,
• Jörg Diendorf,
• Matthias Epple,
• Blair D. Johnston,
• Fritz Krombach,
• Wolfgang G. Kreyling and
• Carsten Schleh

Beilstein J. Nanotechnol. 2013, 4, 933–940, doi:10.3762/bjnano.4.105

• nm with the Cytotoxicity Detection KitPLUS (LDH; Roche Diagnostics, Mannheim, Germany). Both assays were performed according to the manufacturers’ instructions. Quantification of TNF-α and CINC-1 levels in BALF by ELISA TNF-α and cytokine-induced neutrophil chemoattractant-1 (CINC-1) were measured in

Porous polymer coatings as substrates for the formation of high-fidelity micropatterns by quill-like pens

• Michael Hirtz,
• Marcus Lyon,
• Wenqian Feng,
• Andrea E. Holmes,
• Harald Fuchs and
• Pavel A. Levkin

Beilstein J. Nanotechnol. 2013, 4, 377–384, doi:10.3762/bjnano.4.44

• cell analysis) and medical applications in diagnostics such as the detection of pathogens or antibodies. Nitrocellulose films and nylon membranes are widely used as carriers for microarrays, usually with spot sizes in the range of 100 to 500 µm, easily accessible for current inkjet and spotting

Effect of large mechanical stress on the magnetic properties of embedded Fe nanoparticles

• Srinivasa Saranu,
• Sören Selve,
• Ute Kaiser,
• Luyang Han,
• Ulf Wiedwald,
• Paul Ziemann and
• Ulrich Herr

Beilstein J. Nanotechnol. 2011, 2, 268–275, doi:10.3762/bjnano.2.31

• sensors. In this context, it should be noted that magnetic nanoparticles also have applications in other fields, such as medical treatment, diagnostics and imaging [9]. A precise control of the magnetic anisotropy energy is most important for the design of future magnetic data storage media. The total

Structural and magnetic properties of ternary Fe_1–xMn_xPt nanoalloys from first principles

• Markus E. Gruner and
• Peter Entel

Beilstein J. Nanotechnol. 2011, 2, 162–172, doi:10.3762/bjnano.2.20

• technological fields, such as biomedical diagnostics or therapy, catalysis or even mechanical actuation [1][2][3][4][5][6][7][8][9] and have become the focus of much research. Another application, widely discussed in recent years, is in the field of ultra-high density magnetic recording. Here, an exponential

Magnetic nanoparticles for biomedical NMR-based diagnostics

• Huilin Shao,
• Tae-Jong Yoon,
• Monty Liong,
• Ralph Weissleder and
• Hakho Lee

Beilstein J. Nanotechnol. 2010, 1, 142–154, doi:10.3762/bjnano.1.17

• enabled parallel and sensitive measurements to be made from small volume samples. Thus, the DMR technology is a highly attractive platform for portable, low-cost, and efficient biomolecular detection within a biomedical setting. Keywords: biosensor; diagnostics; magnetic nanoparticle; microfluidics

• removed excess unbound MNPs but also retained the BCG bacteria; thus was effective for both concentrating scant bacteria and removing background signal. In comparison to standard TB diagnostics, which involve time-consuming culture and acid-fast bacilli (AFB) smear microscopy, the DMR diagnostic

• ensure detection sensitivity of this assay mode. (Reproduced with permission from [13][14]. Copyright 2002, 2008 Nature Publishing Group.) Higher r2-relaxivity MNPs developed to improve detection sensitivity of in vitro diagnostics. (a) Transmission electron micrograph (TEM) images of manganese-doped