Investigating organic multilayers by spectroscopic ellipsometry: specific and non-specific interactions of polyhistidine with NTA self-assembled monolayers

• Ilaria Solano,
• Pietro Parisse,
• Ornella Cavalleri,
• Federico Gramazio,
• Loredana Casalis and
• Maurizio Canepa

Beilstein J. Nanotechnol. 2016, 7, 544–553, doi:10.3762/bjnano.7.48

• with oligohistidine peptides has been largely implemented over the years for the affinity purification of recombinant proteins [4][5][6], and for the development of bioanalytical surfaces [7][8][9][10][11][12]. To this purpose, self-assembled monolayers (SAMs) terminated with the nitriloacetic acid

Rigid multipodal platforms for metal surfaces

• Michal Valášek,
• Marcin Lindner and
• Marcel Mayor

Beilstein J. Nanotechnol. 2016, 7, 374–405, doi:10.3762/bjnano.7.34

• reported the controlled growth of α-helical peptides on a gold surface modified by this thiol-linking agent. Whitesell, Fox and co-workers used tris(3-sulfanylpropyl)methylamine derivative 1 as an effective linkage for binding surface probes (fluorescent or redox-active) that can be activated by light or

Hemolysin coregulated protein 1 as a molecular gluing unit for the assembly of nanoparticle hybrid structures

• Tuan Anh Pham,
• Andreas Schreiber,
• Elena V. Sturm (née Rosseeva),
• Stefan Schiller and
• Helmut Cölfen

Beilstein J. Nanotechnol. 2016, 7, 351–363, doi:10.3762/bjnano.7.32

• , Habsburgerstrasse 49, D-79104 Freiburg, Germany 10.3762/bjnano.7.32 Abstract Hybrid nanoparticle (NP) structures containing organic building units such as polymers, peptides, DNA and proteins have great potential in biosensor and electronic applications. The nearly free modification of the polymer chain, the

• particular, a template-free approach is more difficult to achieve since specific interactions in terms of chemical and spatial interplay have to be ensured. The controlled assembly of NPs using organic compounds such as polymers [3][4][5][6][7], peptides [8][9] and DNA [10][11][12][13][14] demonstrate great

Time-dependent growth of crystalline Au⁰-nanoparticles in cyanobacteria as self-reproducing bioreactors: 2. Anabaena cylindrica

• Liz M. Rösken,
• Felix Cappel,
• Susanne Körsten,
• Christian B. Fischer,
• Andreas Schönleber,
• Sander van Smaalen,
• Stefan Geimer,
• Christian Beresko,
• Georg Ankerhold and
• Stefan Wehner

Beilstein J. Nanotechnol. 2016, 7, 312–327, doi:10.3762/bjnano.7.30

• [23]. For further use the nanoparticles have to be separated from all the unavoidable molecules present in living organisms, like the biological capping and reducing ligands, e.g., enzymes, chlorophylls, peptides or polyphenols [10][24]. This task is typically quite challenging. Reduction of the toxic

3D solid supported inter-polyelectrolyte complexes obtained by the alternate deposition of poly(diallyldimethylammonium chloride) and poly(sodium 4-styrenesulfonate)

• Eduardo Guzmán,
• Armando Maestro,
• Sara Llamas,
• Jesús Álvarez-Rodríguez,
• Francisco Ortega,
• Ángel Maroto-Valiente and
• Ramón G. Rubio

Beilstein J. Nanotechnol. 2016, 7, 197–208, doi:10.3762/bjnano.7.18

• polyelectrolytes, biopolymers – such as peptides, proteins and nucleic acids – colloidal particles, carbon nanotubes, and/or microgels [8][9][10], which confers to this method an almost unlimited chemical versatility. Even though the method frequently makes use of electrostatic interactions, the multilayers can

Surface-site reactivity in small-molecule adsorption: A theoretical study of thiol binding on multi-coordinated gold clusters

• Elvis C. M. Ting,
• Tatiana Popa and
• Irina Paci

Beilstein J. Nanotechnol. 2016, 7, 53–61, doi:10.3762/bjnano.7.6

• has focused on the binding properties of aminoacids and peptides on metal substrates [1][2][3][4][5][6][7][8][9][10][11][12], due to the relevance of these interactions in device fabrication for biological sensors [13][14][15][16], for in vivo nanoparticle imaging, tagging and tracing [17][18][19][20

Ultrastructural changes in methicillin-resistant Staphylococcus aureus induced by positively charged silver nanoparticles

• Dulce G. Romero-Urbina,
• Humberto H. Lara,
• J. Jesús Velázquez-Salazar,
• M. Josefina Arellano-Jiménez,
• Eduardo Larios,
• Anand Srinivasan,
• Jose L. Lopez-Ribot and
• Miguel José Yacamán

Beilstein J. Nanotechnol. 2015, 6, 2396–2405, doi:10.3762/bjnano.6.246

• vulnerability to cationic antimicrobials, peptides, ions and metals. WTAs retain surface proteins by either covalent or noncovalent attachment [19]. Electron cryomicroscopy studies on S. aureus indicate that WTAs extend over the PG layer. Disregarding their differences, all WTAs maintain anionic backbones

• , and its disruption induces metabolic impairment and cell death. Treating bacteria with AgNPs resulted in an imbalance of the PG layer, as the cells lost the ability to protect themselves against variations in turgor pressure [49]. It is generally accepted that cationic compounds (peptides, antibiotics

Green and energy-efficient methods for the production of metallic nanoparticles

• Mitra Naghdi,
• Mehrdad Taheran,
• Satinder K. Brar,
• M. Verma,
• R. Y. Surampalli and
• J. R. Valero

Beilstein J. Nanotechnol. 2015, 6, 2354–2376, doi:10.3762/bjnano.6.243

Peptide-equipped tobacco mosaic virus templates for selective and controllable biomineral deposition

• Klara Altintoprak,
• Axel Seidenstücker,
• Alexander Welle,
• Sabine Eiben,
• Petia Atanasova,
• Nina Stitz,
• Alfred Plettl,
• Joachim Bill,
• Hartmut Gliemann,
• Holger Jeske,
• Dirk Rothenstein,
• Fania Geiger and
• Christina Wege

Beilstein J. Nanotechnol. 2015, 6, 1399–1412, doi:10.3762/bjnano.6.145

• mosaic virus (TMV) templates have proved particularly suitable towards this goal: Their multivalent protein coating can be modified by high-surface-density conjugation of peptides, inducing and governing silica deposition from precursor solutions in vitro. In this study, TMV has been equipped with

• mineralization-directing peptides designed to yield silica coatings in a reliable and predictable manner via precipitation from tetraethoxysilane (TEOS) precursors. Three peptide groups were compared regarding their influence on silica polymerization: (i) two peptide variants with alternating basic and acidic

• peptides with high ZnO binding affinity. Differential effects on the mineralization of the TMV surface were demonstrated, where a (KD)x charge-relay peptide (designed in this study) led to the most reproducible and selective silica deposition. A homogenous coating of the biotemplate and tight control of

Natural and artificial binders of polyriboadenylic acid and their effect on RNA structure

• Giovanni N. Roviello,
• Domenica Musumeci,
• Valentina Roviello,
• Marina Pirtskhalava,
• Alexander Egoyan and
• Merab Mirtskhulava

Beilstein J. Nanotechnol. 2015, 6, 1338–1347, doi:10.3762/bjnano.6.138

• the interaction with poly(rA) of artificial compounds, such as benzodifuran (Figure 9), cationic peptides and nucleoamino acid-based molecules were found by Roviello et al. [48][49][50][51][52][53][54][55][56], Moggio et al. [57] and Saghyan et al. [58]. In particular, regarding the interaction with

Fulleropeptide esters as potential self-assembled antioxidants

• Mira S. Bjelaković,
• Tatjana J. Kop,
• Jelena Đorđević and
• Dragana R. Milić

Beilstein J. Nanotechnol. 2015, 6, 1065–1071, doi:10.3762/bjnano.6.107

• -assembly has been observed. Here, cryo-transmission electron microscopy [14] and SEM [15] experiments on aqueous solutions of fullerene-based amino acids and peptides confirmed their strong aggregation behavior and formation of spherical and ellipsoidal clusters. In the area of biological applications, a

• , Keller et al. [18] reported the synthesis of fullerenedihydropyrrole cationic peptides, which did not show antibacterial activity. Neuroprotective and antioxidant properties are based on the fact that fullerene derivatives possess an extended π-bond system, with high electron and free-radical species

• , respectively. In addition, the relative molar antioxidant capacities (Aox-rel) of the tested compounds relative to vitamin C are summarized in Table 2. Structures of studied fullereropyrrolidinic (Fp) ester 1 [24] and Fp–peptides 2–12 [27]. Comparison of the relative antioxidant activities (Aox-rel) of the

Morphology control of zinc oxide films via polysaccharide-mediated, low temperature, chemical bath deposition

• Florian Waltz,
• Hans-Christoph Schwarz,
• Andreas M. Schneider,
• Stefanie Eiden and
• Peter Behrens

Beilstein J. Nanotechnol. 2015, 6, 799–808, doi:10.3762/bjnano.6.83

• (malate) [36], ascorbate [37], diaminopropane [38], hexadecyl(trimethyl)azanium bromide (CTAB) [39], and block copolymers [40] have been used for this purpose, in addition to naturally occurring amino acids and peptides [41], which have already been successfully applied in this respect. We recently

Microwave assisted synthesis and characterisation of a zinc oxide/tobacco mosaic virus hybrid material. An active hybrid semiconductor in a field-effect transistor device

• Shawn Sanctis,
• Rudolf C. Hoffmann,
• Sabine Eiben and
• Jörg J. Schneider

Beilstein J. Nanotechnol. 2015, 6, 785–791, doi:10.3762/bjnano.6.81

• defined functional properties [1][2][3]. Significant interest has been dedicated to the generation of multifunctional devices by employing a unique combination of functional biological molecules and inorganic materials. The use of biological building blocks at the nanoscale include DNA, peptides

Tailoring the ligand shell for the control of cellular uptake and optical properties of nanocrystals

• Johannes Ostermann,
• Christian Schmidtke,
• Christopher Wolter,
• Jan-Philip Merkl,
• Hauke Kloust and
• Horst Weller

Beilstein J. Nanotechnol. 2015, 6, 232–242, doi:10.3762/bjnano.6.22

• surrounding the particles fulfills specific requirements [1]. On the one hand the ligands have to stabilize the particles at high dilution in aqueous media, be non-toxic and provide a good shielding against biological conditions (e.g., ions, peptides, enzymes). On the other hand the control over the size and

• type of nanocontainers additional to the subsequently presented strategies. Functionalization properties For the biological use of encapsulated nanoparticles functionalizing of the capsules with relevant molecules (sugars, peptides), proteins or DNA is required. The most common coupling strategies are

Multifunctional layered magnetic composites

• Maria Siglreitmeier,
• Baohu Wu,
• Tina Kollmann,
• Martin Neubauer,
• Gergely Nagy,
• Dietmar Schwahn,
• Vitaliy Pipich,
• Damien Faivre,
• Dirk Zahn,
• Andreas Fery and
• Helmut Cölfen

Beilstein J. Nanotechnol. 2015, 6, 134–148, doi:10.3762/bjnano.6.13

• helix of (Gly–Pro–Hyp)n peptides) is contrasted to ion association to chitin. The latter model was chosen as three poly-(1,4)-D-glucose chains of about 40 Å length (which corresponds to nine monomers) stacked in three layers, which are connected by hydrogen bonds. As a starting point, the association of

Functionalization of α-synuclein fibrils

• Simona Povilonienė,
• Vida Časaitė,
• Virginijus Bukauskas,
• Arūnas Šetkus,
• Juozas Staniulis and
• Rolandas Meškys

Beilstein J. Nanotechnol. 2015, 6, 124–133, doi:10.3762/bjnano.6.12

• Physical Sciences and Technology, A. Gostauto 11, Vilnius LT-01108, Lithuania Institute of Botany of Nature Research Center, Zaliuju Ezeru 49, LT-08406 Vilnius, Lithuania 10.3762/bjnano.6.12 Abstract The propensity of peptides and proteins to form self-assembled structures has very promising applications

• self-assembled structures, amyloid proteins have become a very attractive material in the field of nanobiotechnology [1]. Many proteins or peptides can form amyloids under appropriate experimental conditions and recent studies suggest that amyloid formation is a generic property of the polypeptide

• tissue engineering [27][28], as well as use as a template for fibril metallization [29][30][31][32][33][34][35] or for the biomineralization of fibrils [36]. Nanostructures are usually designed by modifying proteins or peptides prior to fibril assembly [21][37][38][39][40][41]. Although post-assembly

Chemoselective silicification of synthetic peptides and polyamines

• Maryna Abacilar,
• Fabian Daus and
• Armin Geyer

Beilstein J. Nanotechnol. 2015, 6, 103–110, doi:10.3762/bjnano.6.10

• present experiments which quantify the additivity, and potential cooperativity of long chain polyamines (LCPAs) and cationic peptides in the silicification process. Keywords: biomineralisation; biosilicification; NMR spectroscopy; polyamines; silaffin; Introduction Modifications of the Stöber method [1

• components at the surface of the forming silica beads. Poulsen et al. investigated the mutual influence of peptides and LCPAs. Here we investigate the simplest scenario of chemoselective precipitation, which is the differentiation between two dissolved components, a cationic peptide and an oligoamine, that

• with the less capable Si precipitator in the presence of the better precipitator? Is there a measurable cooperativity between peptides and amines? Silica precipitation experiments are time consuming and error-prone because many actions are needed to separate the precipitate of amorphous silica, to dry

Inorganic Janus particles for biomedical applications

• Isabel Schick,
• Steffen Lorenz,
• Dominik Gehrig,
• Stefan Tenzer,
• Wiebke Storck,
• Karl Fischer,
• Dennis Strand,
• Frédéric Laquai and
• Wolfgang Tremel

Beilstein J. Nanotechnol. 2014, 5, 2346–2362, doi:10.3762/bjnano.5.244

• peptides [112]. Consequently, this synthetic route allows precise control over nanoparticle composition, domain sizes, surface functionalization, and, also, surface charge. Biomedical applications When nanoparticles get in contact with body fluids, a variety of serum components binds to the surface of

• adsorption to silica particles of varying sizes and surface functionalization, it could be shown that small changes also are sufficient to drastically change to the affinity for peptides [116][117][118]. As a consequence, it is possible that slight structural differences of the silica shell of MnO and Fe3O4

Coating with luminal gut-constituents alters adherence of nanoparticles to intestinal epithelial cells

• Heike Sinnecker,
• Katrin Ramaker and
• Andreas Frey

Beilstein J. Nanotechnol. 2014, 5, 2308–2315, doi:10.3762/bjnano.5.239

• showed that the decrease in fluorescence at the cell surface was not associated with an increased fluorescence inside the cells, indicating that the protein coat had not led to an enhanced NP uptake by the cells. When analyzing the effect of NP treatment with meat extract, a mixture of peptides and amino

• acids representative for an alimentary peptide source, we noticed that this seemed to have no substantial effect on the adherence of NPs to the cells, only with the 20 nm NPs slightly higher fluorescence intensities were measured (Figure 2A). Individual peptides and amino acids of this extract do

Localized surface plasmon resonances in nanostructures to enhance nonlinear vibrational spectroscopies: towards an astonishing molecular sensitivity

• Dan Lis and
• Francesca Cecchet

Beilstein J. Nanotechnol. 2014, 5, 2275–2292, doi:10.3762/bjnano.5.237

• working with a molecular concentration 104 times lower. This ultra-sensitive detection of non-labelled peptides achieved with SE-CARS is a brilliant example of single molecular detection and offers great perspectives for biomedical detection with very low molecular concentration threshold. Schlücker et al

Biopolymer colloids for controlling and templating inorganic synthesis

• Laura C. Preiss,
• Katharina Landfester and
• Rafael Muñoz-Espí

Beilstein J. Nanotechnol. 2014, 5, 2129–2138, doi:10.3762/bjnano.5.222

• peptides. In situ formation of the inorganic material After having briefly discussed a few examples in which the inorganic material is formed ex situ and combined a posteriori with biopolymers, we will revise now in situ strategies, with biopolymers playing an active role during the formation of inorganic

•  2) [38]. In a more recent work, we have also shown that charged acidic peptides are able to stabilize vaterite, and we studied the effect of the acidity of the amino acid residues on this stabilization [39]. In a previous work, synthetic polymer colloidal particles functionalized with different

• to the surface of colloidal particles. In this sense, Krattiger et al. [44] reported the morphogenesis of CaCO3 and DL-alanine crystals in the presence of polystyrene beads functionalized with synthetic peptides with different amino acids and oligopeptides. B. Biopolymers as “supports” B1. Molecular

Electrostatic interplay: The interaction triangle of polyamines, silicic acid, and phosphate studied through turbidity measurements, silicomolybdic acid test, and ²⁹Si NMR spectroscopy

• Anne Jantschke,
• Katrin Spinde and
• Eike Brunner

Beilstein J. Nanotechnol. 2014, 5, 2026–2035, doi:10.3762/bjnano.5.211

• ]. They consist of linear oligo-propyleneimine chains attached to putrescine or spermine [5][6]. Biosilica-associated LCPAs occur either as free molecules [1][4] or covalently attached to the ε-amino groups of certain lysine-residues [7][8] in highly post-translationally modified peptides, so-called

Carbon-based smart nanomaterials in biomedicine and neuroengineering

• Antonina M. Monaco and
• Michele Giugliano

Beilstein J. Nanotechnol. 2014, 5, 1849–1863, doi:10.3762/bjnano.5.196

• consists of coating CNTs with surfactants, peptides, polymers, or nucleic acids, which preserve their aromatic structure, and (ii) the covalent approach [37][38][39], by means of applying several protocols, such as oxidation in strong acids, fluorination [40], and Bingel [41] and Billups [42] reactions

Real-time monitoring of calcium carbonate and cationic peptide deposition on carboxylate-SAM using a microfluidic SAW biosensor

• Anna Pohl and
• Ingrid M. Weiss

Beilstein J. Nanotechnol. 2014, 5, 1823–1835, doi:10.3762/bjnano.5.193

• the interaction of calcium carbonate with standard carboxylate self-assembled monolayer sensor chips. Different fluids, with and without biomolecular components, were investigated. The pH-dependent surface interactions of two bio-inspired cationic peptides, AS8 and ES9, which are similar to an

• organic biomolecules and calcium carbonate in the presence of carboxylate surfaces could be reproducibly quantified in real-time assays. Here, we report a case study with calcium carbonate, both in pure aqueous systems and in the presence of citric acid. We also investigated the two cationic peptides ES9

• detail with the particular aim to inspire similar investigations in biomineralization research in the future. A standard database of the function of organic molecules, peptides and proteins in mineralizing systems will be a major achievement of global significance for materials science, biomedical

Non-covalent and reversible functionalization of carbon nanotubes

• Antonello Di Crescenzo,
• Valeria Ettorre and
• Antonella Fontana

Beilstein J. Nanotechnol. 2014, 5, 1675–1690, doi:10.3762/bjnano.5.178

• favorably exploited for CNTs precipitation/dispersion cycles. Ortiz-Acevedo et al. obtained a diameter-selective solubilization of SWCNTs by using cyclic peptides containing thiol groups [101]. The oxidation induces polymerization of the peptides which wrap around the nanotube achieving a size-selective