Distance dependence of near-field fluorescence enhancement and quenching of single quantum dots

• Volker Walhorn,
• Jan Paskarbeit,
• Heinrich Gotthard Frey,
• Alexander Harder and
• Dario Anselmetti

Beilstein J. Nanotechnol. 2011, 2, 645–652, doi:10.3762/bjnano.2.68

• Volker Walhorn Jan Paskarbeit Heinrich Gotthard Frey Alexander Harder Dario Anselmetti Experimental Biophysics and Applied Nanosciences, Bielefeld University, Department of Physics, Universitätsstr. 25, 33615 Bielefeld, Germany 10.3762/bjnano.2.68 Abstract In fluorescence microscopy and

• effects. In the presented work we used a combined total internal reflection fluorescence microscopy (TIRFM)–AFM setup to elucidate this issue. We measured the fluorescence emission emanating from single quantum dots as a function of distance from the apex of a gold-coated cantilever tip. As well as

• Fluorescence microscopy and spectroscopy are important and versatile tools in life sciences. Fluorophores are not merely position markers, but can be regarded as active transducers that interact with species in their local vicinity and provide information about their micro-environment. The spectroscopic

Towards multiple readout application of plasmonic arrays

• Dana Cialla,
• Karina Weber,
• René Böhme,
• Uwe Hübner,
• Henrik Schneidewind,
• Matthias Zeisberger,
• Roland Mattheis,
• Robert Möller and
• Jürgen Popp

Beilstein J. Nanotechnol. 2011, 2, 501–508, doi:10.3762/bjnano.2.54

• spectrum of the fluorescence reporter molecules prevents the parallel detection of several fluorescent dye labels by fluorescence microscopy. Raman spectroscopy, i.e., the excitation of vibrational modes through inelastic light scattering, allows one to obtain highly specific molecular fingerprint

• on a quartz wafer biochip platform for DNA detection by fluorescence and SERS readout. By doing so, fluorescence microscopy allows for a fast detection of any positive or negative binding event within several seconds. Moreover SERS provides detailed molecular fingerprint information of fluorescence

Characterization of protein adsorption onto FePt nanoparticles using dual-focus fluorescence correlation spectroscopy

• Pauline Maffre,
• Karin Nienhaus,
• Faheem Amin,
• Wolfgang J. Parak and
• G. Ulrich Nienhaus

Beilstein J. Nanotechnol. 2011, 2, 374–383, doi:10.3762/bjnano.2.43

• understand the structural and dynamic properties of the protein corona at the molecular level. Recently, we have used quantitative fluorescence microscopy, especially fluorescence correlation spectroscopy (FCS), to study protein adsorption of human serum albumin (HSA) on polymer-coated FePt NPs with an