Theranostic potential of self-luminescent branched polyethyleneimine-coated superparamagnetic iron oxide nanoparticles

• Rouhollah Khodadust,
• Ozlem Unal and
• Havva Yagci Acar

Beilstein J. Nanotechnol. 2022, 13, 82–95, doi:10.3762/bjnano.13.6

• content of the particles was determined by thermogravimetric analysis (TGA). Erbitux conjugation to SPION@bPEI Before any application, Erbitux (Erb) (Merck Serono, UK) with a stock concentration of 5 mg/mL was washed with phosphate-buffered saline (PBS) (Biomatik, Canada) using an ultracentrifugation

• between the negative charge (due to the phosphate group of dsRNA/poly I:C) and the positive charge of the nanoparticles (due to primary amine groups of polyethyleneimine). This is due to the fact that the highly positively charged PEI electrostatically condenses high molecular weight DNA or RNA to

Alteration of nanomechanical properties of pancreatic cancer cells through anticancer drug treatment revealed by atomic force microscopy

• Xiaoteng Liang,
• Shuai Liu,
• Xiuchao Wang,
• Dan Xia and
• Qiang Li

Beilstein J. Nanotechnol. 2021, 12, 1372–1379, doi:10.3762/bjnano.12.101

• increasing DOX concentrations was shown. This study may be conducive regarding innovations in cancer prevention, diagnosis methods, and the application of drug screening. Materials and Methods Materials Dulbecco’s modified eagle medium (DMEM), RPMI-1640 and phosphate-buffered saline (PBS) were obtained from

Biocompatibility and cytotoxicity in vitro of surface-functionalized drug-loaded spinel ferrite nanoparticles

• Sadaf Mushtaq,
• Khuram Shahzad,
• Tariq Saeed,
• Anwar Ul-Hamid,
• Bilal Haider Abbasi,
• Nafees Ahmad,
• Waqas Khalid,
• Muhammad Atif,
• Zulqurnain Ali and
• Rashda Abbasi

Beilstein J. Nanotechnol. 2021, 12, 1339–1364, doi:10.3762/bjnano.12.99

• specificity with significantly higher (p < 0.005) drug release in an acidic environment (pH 5.5). The nanoparticles were highly colloidal (zeta potential = −35 to −26 mV) in deionized water, phosphate buffer saline (PBS), and sodium borate buffer (SBB). They showed elevated and dose-dependent cytotoxicity in

• ) dispersed in deionized water, sodium borate buffer (SBB) pH 9.0, phosphate buffer saline (PBS) pH 7.4, and DMEM were used for zeta potential measurements. The purpose of using buffers (i.e., SBB, PBS) was to get indirect surface charge information. Deionized water was used to check the influence of

pH-driven enhancement of anti-tubercular drug loading on iron oxide nanoparticles for drug delivery in macrophages

• Karishma Berta Cotta,
• Sarika Mehra and
• Rajdip Bandyopadhyaya

Beilstein J. Nanotechnol. 2021, 12, 1127–1139, doi:10.3762/bjnano.12.84

• neutral (pH 7.4) and acidic (pH 5) conditions. To investigate any alterations in the drug release profile from the NOR@IONPs based on the cellular pH variations, the drug release was monitored over 48 h in phosphate buffer saline (PBS) release media, maintaining the pH at either 5 or 7.4. We observed that

• correcting the volume reduction due to sampling loss. The concentration of NOR released was estimated fluorometrically (Ex/Em: 280/420 nm) (SpectraMax M5, Molecular Devices) using a known NOR concentration versus fluorescence standard, prepared with dilution in phosphate buffer saline (PBS) (same as the

Progress and innovation of nanostructured sulfur cathodes and metal-free anodes for room-temperature Na–S batteries

• Marina Tabuyo-Martínez,
• Bernd Wicklein and
• Pilar Aranda

Beilstein J. Nanotechnol. 2021, 12, 995–1020, doi:10.3762/bjnano.12.75

• ][83]. The majority of the alloy anodes discussed in this section were tested and employed in sodium-ion batteries with diverse types of oxide and phosphate cathodes. However, they are now also increasingly incorporated in sodium-ion–sulfur batteries. Lee et al. were amongst the first to proof this

Modification of a SERS-active Ag surface to promote adsorption of charged analytes: effect of Cu²⁺ ions

• Bahdan V. Ranishenka,
• Andrei Yu. Panarin,
• Irina A. Chelnokova,
• Sergei N. Terekhov,
• Peter Mojzes and
• Vadim V. Shmanai

Beilstein J. Nanotechnol. 2021, 12, 902–912, doi:10.3762/bjnano.12.67

• of different modifications, only treatment with copper ions appeared to be successful (Figure 8, numbers 1 and 2). According to an earlier investigation [35], the Cu2+ ions are able to bind effectively both the phosphate groups and the nucleobases in the oligonucleotide molecules to yield charge

Comprehensive review on ultrasound-responsive theranostic nanomaterials: mechanisms, structures and medical applications

• Sepand Tehrani Fateh,
• Lida Moradi,
• Elmira Kohan,
• Michael R. Hamblin and
• Amin Shiralizadeh Dezfuli

Beilstein J. Nanotechnol. 2021, 12, 808–862, doi:10.3762/bjnano.12.64

Fate and transformation of silver nanoparticles in different biological conditions

• Barbara Pem,
• Marija Ćurlin,
• Darija Domazet Jurašin,
• Valerije Vrček,
• Rinea Barbir,
• Vedran Micek,
• Raluca M. Fratila,
• Jesus M. de la Fuente and
• Ivana Vinković Vrček

Beilstein J. Nanotechnol. 2021, 12, 665–679, doi:10.3762/bjnano.12.53

• synthesis of AgNPs in the presence of GSH was performed by mixing appropriate amounts of AgNO3 and GSH to the final concentrations of 20 and 10 mM, respectively, in 25 mM of phosphate buffer solution (K2HPO4/KH2PO4, 0.401:0.014, w/w [g]). The reaction mixture was left to react for 2 h at room temperature

• the respective HAADF–STEM images. 1H NMR spectra of (a) GSH-coated AgNPs formed during the interaction between AgNO3 and GSH and (b) free GSH. Both spectra are obtained in 25 mM phosphate buffer (pH 6.8). The structure of reduced GSH is shown above panel (a). Biotransformation patterns of AgNPs in

On the stability of microwave-fabricated SERS substrates – chemical and morphological considerations

• Limin Wang,
• Aisha Adebola Womiloju,
• Christiane Höppener,
• Ulrich S. Schubert and
• Stephanie Hoeppener

Beilstein J. Nanotechnol. 2021, 12, 541–551, doi:10.3762/bjnano.12.44

• sulfoxide (DMSO) treatment completely preserved or even slightly improved the Raman enhancement capabilities. SERS substrates immersed into phosphate-buffered saline (PBS) solutions were observed to be rather instable in low and neutral pH buffer solutions. Other buffer systems showed less severe influences

• choice of a suitable media is of utmost importance for many biological investigations as it can alter the structure of biomolecules (e.g., proteins) [33]. We tested five commonly used buffer systems which include phosphate-buffered saline (PBS), HEPES-buffered glucose solution (HBG), tris-borate-EDTA

The preparation temperature influences the physicochemical nature and activity of nanoceria

• Robert A. Yokel,
• Wendel Wohlleben,
• Johannes Georg Keller,
• Matthew L. Hancock,
• Jason M. Unrine,
• D. Allan Butterfield and
• Eric A. Grulke

Beilstein J. Nanotechnol. 2021, 12, 525–540, doi:10.3762/bjnano.12.43

• . Nanoceria dissolution releases Ce3+ ions, which, with phosphate, form insoluble cerium phosphate in vivo. The addition of immobilized phosphates did not accelerate nanoceria dissolution, suggesting that the Ce3+ ion release during nanoceria dissolution was phosphate-independent. Smaller particles resulting

• applications), the influence of carboxylic acids and immobilized phosphate on their dissolution, the influence of nanoceria after partial dissolution on a selected biological effect, and the surface reactivity. Based on reports of very limited NM-212 dissolution and physicochemical differences between

• stable complexes with phosphate but phosphate inhibits nanoceria dissolution, it was hypothesized that phosphates not in direct contact with nanoceria would create a sink for released cerium ions and increase the nanoceria dissolution rate. Assuming that NM-212 was calcined to a crystalline form with

The impact of molecular tumor profiling on the design strategies for targeting myeloid leukemia and EGFR/CD44-positive solid tumors

• Nikola Geskovski,
• Nadica Matevska-Geshkovska,
• Simona Dimchevska Sazdovska,
• Marija Glavas Dodov,
• Kristina Mladenovska and
• Katerina Goracinova

Beilstein J. Nanotechnol. 2021, 12, 375–401, doi:10.3762/bjnano.12.31

• identified in relapsed CML patients receiving imatinib. However, the most frequent alterations occur in three different regions and seven specific residues, that is, in the docking site for phosphate moieties of ATP named P-loop (M244V, G250E, Y253F/H, and E255K/V), in the contact site related to selective

Doxorubicin-loaded gold nanorods: a multifunctional chemo-photothermal nanoplatform for cancer management

• Uzma Azeem Awan,
• Abida Raza,
• Shaukat Ali,
• Rida Fatima Saeed and
• Nosheen Akhtar

Beilstein J. Nanotechnol. 2021, 12, 295–303, doi:10.3762/bjnano.12.24

• analyzed by UV–vis spectroscopy. In vitro drug release by NIR exposure NIR-triggered drug release from PSS-GNRs was measured in 10 mM phosphate-buffered saline (PBS, pH 5.6 at 37 °C). A continuous-wave 808 nm NIR laser (Ti-Sapphire, Spectra Physics CA 95054, USA) was used. DOX-PSS-GNRs (40 µg/mL, 2 mL

• collection was performed by a trained phlebotomist in order to minimize the risk to the donor. A written informed consent was obtained from each donor prior to the blood drawn. To the 5 mL of blood 15 mL of sterilized phosphate buffer saline (PBS) was added and, after slow agitation, tubes were centrifuged

Characterization, bio-uptake and toxicity of polymer-coated silver nanoparticles and their interaction with human peripheral blood mononuclear cells

• Sahar Pourhoseini,
• Reilly T. Enos,
• Angela E. Murphy,
• Bo Cai and
• Jamie R. Lead

Beilstein J. Nanotechnol. 2021, 12, 282–294, doi:10.3762/bjnano.12.23

• Healthcare Bio-Sciences AB, Sweden) [65][66]. In brief, peripheral blood from healthy volunteers was diluted with an equal volume of sterile phosphate-buffered saline (PBS; Gibco by life Technologies) and slowly layered onto the Ficoll-Paque media solution. Tubes were centrifuged at 500g for 30 to 40 min at

Differences in surface chemistry of iron oxide nanoparticles result in different routes of internalization

• Barbora Svitkova,
• Vlasta Zavisova,
• Veronika Nemethova,
• Martina Koneracka,
• Miroslava Kretova,
• Filip Razga,
• Monika Ursinyova and
• Alena Gabelova

Beilstein J. Nanotechnol. 2021, 12, 270–281, doi:10.3762/bjnano.12.22

• additional time of 1 h. Cell exposure was finished by sucking off the culture medium and washing the cells twice with phosphate-buffered saline (PBS). An illustration of the treatment is shown in Figure 4. The final concentrations of individual inhibitors of endocytosis or cytoskeleton dynamics were prepared

• ) with endocytic inhibitor for 1 h in medium with 2% FBS, then (time 1) fresh medium with PEG-SO-MNPs or BSA-SO-MNPs in the presence of the same inhibitor was added for another period of 1 h. Cell exposure was finished by sucking off the culture medium (time 2) and washing the cells twice with phosphate

The nanomorphology of cell surfaces of adhered osteoblasts

• Christian Voelkner,
• Mirco Wendt,
• Regina Lange,
• Max Ulbrich,
• Martina Gruening,
• Susanne Staehlke,
• Barbara Nebe,
• Ingo Barke and
• Sylvia Speller

Beilstein J. Nanotechnol. 2021, 12, 242–256, doi:10.3762/bjnano.12.20

• Osteoblasts are bone-mineralizing cells situated inside the matrix boundaries of the osteoid. They can release matrix vesicles containing calcium and phosphate, eventually leading to precipitation and growth of bone mineral [1][2]. They adhere to and spread on a wide spectrum of pristine and coated material

• % CO2, 37 °C) was used. The sample was immersed in physiological electrolyte (DMEM with 10% FCS and 1% gentamicin) in case of living topography and membrane fluctuation measurements. Measurements of fixed cells took place at room temperature (21 °C) in phosphate-buffered saline (PBS). Nanopipettes with

A review on the green and sustainable synthesis of silver nanoparticles and one-dimensional silver nanostructures

• Sina Kaabipour and
• Shohreh Hemmati

Beilstein J. Nanotechnol. 2021, 12, 102–136, doi:10.3762/bjnano.12.9

• amorphous calcium phosphate particles for enhanced adhesive applications [124]. The spray pyrolysis process requires using an atomizer, a tube furnace, a reaction tube, a collection filter, and a vacuum pump [124]. This method is also often used for production of metal powders and demonstrates less

Effect of different silica coatings on the toxicity of upconversion nanoparticles on RAW 264.7 macrophage cells

• Cynthia Kembuan,
• Helena Oliveira and
• Christina Graf

Beilstein J. Nanotechnol. 2021, 12, 35–48, doi:10.3762/bjnano.12.3

• lanthanide chlorides in DMEM. However, only Er3+ could be detected, with a high measurement uncertainty, in the filtrate of lanthanide chloride solutions. Lanthanide ions are known to bind to phosphate in phosphate-buffered saline (PBS) and form stable lanthanide phosphates [20]. Since DMEM contains Na2HPO4

Atomic layer deposited films of Al₂O₃ on fluorine-doped tin oxide electrodes: stability and barrier properties

• Hana Krýsová,
• Michael Neumann-Spallart,
• Hana Tarábková,
• Pavel Janda,
• Ladislav Kavan and
• Josef Krýsa

Beilstein J. Nanotechnol. 2021, 12, 24–34, doi:10.3762/bjnano.12.2

• grade. 0.1 M sodium phosphate buffer solution, pH 7.2, was obtained from Fluka. Triply distilled water was used for the preparation of solutions. Fluorine-doped tin oxide-coated 2 mm thick glass (TEC-7, 7 Ω/□, Sigma-Aldrich), was ultrasonically pre-cleaned with isopropanol, treated with acetone, ethanol

• M KCl) and a platinum rod was used as the counter electrode. The blocking properties of the deposited layers were evaluated by cyclic voltammetry (CV) in an aqueous electrolyte composed of 0.5 mM K3[Fe(CN)6] and 0.5 mM K4[Fe(CN)6] in 0.5 M KCl (pH 2.5, adjusted with HCl) or in phosphate buffer (pH

• (free of Prussian blue) was observed when the Al2O3-protected electrode was denuded by the dissolution of its coating, both in acidic (Figure 6) and alkaline (Figure 4) media. Exposure to phosphate buffer (pH 7.2) Cyclic voltammetry curves of 17 nm thick Al2O3 films on FTO substrates (in the presence of

Bio-imaging with the helium-ion microscope: A review

• Matthias Schmidt,
• James M. Byrne and
• Ilari J. Maasilta

Beilstein J. Nanotechnol. 2021, 12, 1–23, doi:10.3762/bjnano.12.1

• promising tool to study intracellular deposits of certain chemical elements. An example are phosphate granules in algal biofilms, which were previously investigated using multiple microscopes in a correlative study [46]. Another, certainly more challenging, example would be the identification of iron–sulfur

Cardiomyocyte uptake mechanism of a hydroxyapatite nanoparticle mediated gene delivery system

• Hiroaki Komuro,
• Masahiro Yamazoe,
• Kosuke Nozaki,
• Akiko Nagai and
• Tetsuo Sasano

Beilstein J. Nanotechnol. 2020, 11, 1685–1692, doi:10.3762/bjnano.11.150

• regarding safety has attracted significant attention [4][5]. The calcium phosphate (CaP) co-precipitation method has been extensively used for gene delivery due to its excellent biocompatibility and simple preparation [6]. CaP is commonly considered as one of the most important inorganic materials for

• , this method may provide a solution to the current problem of using calcium phosphate. The cellular uptake performance is important for a successful vector-mediated gene transfection. In the cellular uptake process, the internalization pathway is an essential factor to prevent the fate of lysosomal

• . This material was found to correspond to the B-type carbonate-containing HAp in which the phosphate group was substituted by the carbonate group absorption band in the FTIR spectrum. The carbonate amount, including the HAp crystalline structure that was calculated in a previous report, was

Electrokinetic characterization of synthetic protein nanoparticles

• Daniel F. Quevedo,
• Cody J. Lentz,
• Adriana Coll de Peña,
• Yazmin Hernandez,
• Nahal Habibi,
• Rikako Miki,
• Joerg Lahann and
• Blanca H. Lapizco-Encinas

Beilstein J. Nanotechnol. 2020, 11, 1556–1567, doi:10.3762/bjnano.11.138

• measured in Dulbecco’s phosphate-buffered saline (DPBS), they had significantly different values for ζp, which were −3.5 ± 0.5 mV and 11.2 ± 0.9 mV for SPNP-BSA-488s and SPNP-Lys-488s, respectively. SPNP-Lys-488s were then analyzed using an EK device, as previously described, and the particles were trapped

Cu₂O nanoparticles for the degradation of methyl parathion

• Juan Rizo,
• David Díaz,
• Benito Reyes-Trejo and
• M. Josefina Arellano-Jiménez

Beilstein J. Nanotechnol. 2020, 11, 1546–1555, doi:10.3762/bjnano.11.137

• ]. The 31P NMR spectra of Figure 4 show the results obtained when Cu2O NPs with an approximate size of 29 nm were used for the degradation. In this case, the final product formed is dimethyl hydrogen phosphate with a chemical shift of −4.9 ppm in deuterated chloroform (CDCl3). Similarly, the chemical

• shift of 42.2 ppm is that of dimethyl phosphorothioate (P=S) [40], which then hydrolyzes after 44 h to form dimethyl hydrogen phosphate (P=O) and the NMR peak at −4.9 ppm developes [42]. The intensity of the chemical shift is relatively low due to the low solubility in CDCl3, but when D2O is used the

• -nitrophenol. The peaks at 3.45 and 3.48 ppm are the methyl groups of phosphate, which show coupling to phosphorous, and the peak at 4.65 ppm is due to the HDO produced by the deuterium interchange with the hydroxyl group of 4-nitrophenol. D2O was used as solvent for 1H NMR instead, of CDCl3 like in 31P NMR

Antimicrobial metal-based nanoparticles: a review on their synthesis, types and antimicrobial action

• Matías Guerrero Correa,
• Fernanda B. Martínez,
• Cristian Patiño Vidal,
• Camilo Streitt,
• Juan Escrig and
• Carol Lopez de Dicastillo

Beilstein J. Nanotechnol. 2020, 11, 1450–1469, doi:10.3762/bjnano.11.129

• , transcription, and cell division since ROS can generate DNA mutations. These modifications may target the sugar-phosphate or the nucleobases and cause saccharide fragmentation and strand break [160]. This cleavage induced by the nanoparticles was studied in the pBR322 plasmid in the presence of Ag NPs via

Transient coating of γ-Fe₂O₃ nanoparticles with glutamate for its delivery to and removal from brain nerve terminals

• Konstantin Paliienko,
• Artem Pastukhov,
• Michal Babič,
• Daniel Horák,
• Olga Vasylchenko and
• Tatiana Borisova

Beilstein J. Nanotechnol. 2020, 11, 1381–1393, doi:10.3762/bjnano.11.122

• phosphate ions. Also, HEPES affected the adsorption of glutamate/ʟ-[14C]glutamate on the nanoparticle surface, but to a lesser extent. It was confirmed by these adsorption experiments that changes in synaptosomal ʟ-[14C]glutamate uptake and the ambient level were not associated with the adsorption of

Ultrasensitive detection of cadmium ions using a microcantilever-based piezoresistive sensor for groundwater

• Dinesh Rotake,
• Anand Darji and
• Nitin Kale

Beilstein J. Nanotechnol. 2020, 11, 1242–1253, doi:10.3762/bjnano.11.108

• phosphate buffer saline (PBS, pH 7) according to [34] was prepared. The cysteamine SAM was allowed to cross-link with the ᴅʟ-glyceraldehyde solution for at least 2–3 hours by covering the container using silver foil. Stock solutions of 1 mM/10 mL of different salts (AlCl3, MnCl2, CrCl3, HgCl2, PbCl2, CdCl2