Search results

Search for "absorbance" in Full Text gives 377 result(s) in Beilstein Journal of Nanotechnology. Showing first 200.

Preparation and in vivo evaluation of glyco-gold nanoparticles carrying synthetic mycobacterial hexaarabinofuranoside

  • Gennady L. Burygin,
  • Polina I. Abronina,
  • Nikita M. Podvalnyy,
  • Sergey A. Staroverov,
  • Leonid O. Kononov and
  • Lev A. Dykman

Beilstein J. Nanotechnol. 2020, 11, 480–493, doi:10.3762/bjnano.11.39

Graphical Abstract
  • contents. The solution was allowed to cool by incubation at room temperature for 15 min. Absorbance was measured in 1 cm quartz cuvettes on a Specord 250 spectrophotometer (Analytik, Jena, Germany) at 490 nm using solutions of the parent glycoside 1 for calibration. The calculation also took into
  • reaction was stopped with 100 μL of 1 N H2SO4. The absorbance at 492 nm was read on a Multiskan Ascent analyzer (Thermo). Absorbance values at 492 nm for the wells without bacterial cells were using as the control. Data were processed with Microsoft Excel 2003 software (Microsoft Corp.); 95% confidence
PDF
Album
Supp Info
Full Research Paper
Published 19 Mar 2020

Interfacial charge transfer processes in 2D and 3D semiconducting hybrid perovskites: azobenzene as photoswitchable ligand

  • Nicole Fillafer,
  • Tobias Seewald,
  • Lukas Schmidt-Mende and
  • Sebastian Polarz

Beilstein J. Nanotechnol. 2020, 11, 466–479, doi:10.3762/bjnano.11.38

Graphical Abstract
  • only discuss the results for AzoC2, all other ligands behave very similarly and the data can be found in Supporting Information File 1, Figures S10–S12. A solution of AzoC2 in purified H2O (c = 10−5 M) was irradiated at 313 nm and the absorbance was measured at certain time intervals. The absorption
PDF
Album
Supp Info
Full Research Paper
Published 17 Mar 2020

Electrochemically derived functionalized graphene for bulk production of hydrogen peroxide

  • Munaiah Yeddala,
  • Pallavi Thakur,
  • Anugraha A and
  • Tharangattu N. Narayanan

Beilstein J. Nanotechnol. 2020, 11, 432–442, doi:10.3762/bjnano.11.34

Graphical Abstract
  • H2O2. The H2O2 produced through ORR is also quantified using a Ce(SO4)2 solution assisted UV–visible absorbance based analysis, where the details and calibration are given in Supporting Information File 1. Figure 7a shows chronoamperograms of ORR on EEG-coated graphite papers at 0.358 V vs RHE in 0.1 M
PDF
Album
Supp Info
Full Research Paper
Published 09 Mar 2020

Nanoparticles based on the zwitterionic pillar[5]arene and Ag+: synthesis, self-assembly and cytotoxicity in the human lung cancer cell line A549

  • Dmitriy N. Shurpik,
  • Denis A. Sevastyanov,
  • Pavel V. Zelenikhin,
  • Pavel L. Padnya,
  • Vladimir G. Evtugyn,
  • Yuriy N. Osin and
  • Ivan I. Stoikov

Beilstein J. Nanotechnol. 2020, 11, 421–431, doi:10.3762/bjnano.11.33

Graphical Abstract
  • ) was added to the wells. The plates were incubated at 37 °C for 4 h. Then the medium was aspirated from the wells and 100 μL of DMSO (to dissolve the crystals) was added to each well. The probes were incubated at 37 °C for 5 min. The absorbance was measured at 570 nm using a xMark spectrophotometer
PDF
Album
Supp Info
Full Research Paper
Published 05 Mar 2020

Synthesis and enhanced photocatalytic performance of 0D/2D CuO/tourmaline composite photocatalysts

  • Changqiang Yu,
  • Min Wen,
  • Zhen Tong,
  • Shuhua Li,
  • Yanhong Yin,
  • Xianbin Liu,
  • Yesheng Li,
  • Tongxiang Liang,
  • Ziping Wu and
  • Dionysios D. Dionysiou

Beilstein J. Nanotechnol. 2020, 11, 407–416, doi:10.3762/bjnano.11.31

Graphical Abstract
  • clarified by centrifugation for the measurement of absorbance, which can be used to monitor the progress of the photocatalytic degradation reactions. For the durability test, the used photocatalyst was firstly rinsed with ethanol and deionized water by centrifugation, then it was collected by using aqueous
PDF
Album
Supp Info
Full Research Paper
Published 02 Mar 2020

Brome mosaic virus-like particles as siRNA nanocarriers for biomedical purposes

  • Alfredo Nuñez-Rivera,
  • Pierrick G. J. Fournier,
  • Danna L. Arellano,
  • Ana G. Rodriguez-Hernandez,
  • Rafael Vazquez-Duhalt and
  • Ruben D. Cadena-Nava

Beilstein J. Nanotechnol. 2020, 11, 372–382, doi:10.3762/bjnano.11.28

Graphical Abstract
  • calculated by measuring the UV absorbance using a Nanodrop 2000c spectrophotometer (ThermoFisher Scientific). All the procedures were performed at 4 °C. Finally, the purified viruses were stored at −80 °C in 1.5 mL microcentrifuge tubes. Cell cultures The breast tumor cell lines MDA-MB-231 and MCF-7 were
  • virions per cell), PEGylated or non-functionalized capsids, or as positive control lipopolysaccharides (2.5 or 75 ng/mL). Then, 50 µL of supernatant was transferred into a new 96-well plate and 150 µL of Quantity Blue (InvivoGen) reagent was added and incubated at 37 °C for 1 h. The absorbance at 655 nm
PDF
Album
Supp Info
Full Research Paper
Published 20 Feb 2020

Poly(1-vinylimidazole) polyplexes as novel therapeutic gene carriers for lung cancer therapy

  • Gayathri Kandasamy,
  • Elena N. Danilovtseva,
  • Vadim V. Annenkov and
  • Uma Maheswari Krishnan

Beilstein J. Nanotechnol. 2020, 11, 354–369, doi:10.3762/bjnano.11.26

Graphical Abstract
  • incubation for specified periods of time (24 h or 48 h). MTS reagent (20 µL) along with 200 µL of serum-free media was added to each sample well and incubated at 37 °C for 2 h. The reaction was terminated with 10% sodium dodecyl sulfate (SDS) solution. The absorbance was read at 490 nm using a multimode
PDF
Album
Full Research Paper
Published 17 Feb 2020

Using gold nanoparticles to detect single-nucleotide polymorphisms: toward liquid biopsy

  • María Sanromán Iglesias and
  • Marek Grzelczak

Beilstein J. Nanotechnol. 2020, 11, 263–284, doi:10.3762/bjnano.11.20

Graphical Abstract
  • gold nanoparticles are their optical properties, which yield an exceptional light absorbance in the visible spectral range. This is explained by the fact that in the metallic core, the conductive electrons experience coherent oscillations in the presence of incoming electromagnetic radiation, thereby
  • nanoparticle amplification. b) Optical characterization of the assay solution spiked with different amounts of c.2573 T>G mutant EGFR gene in the presence of a background wild-type EGFR sequence, showing a specificity down to 1%. c) The relation of increased absorbance with increasing the relative
PDF
Album
Review
Published 31 Jan 2020

Recent progress in perovskite solar cells: the perovskite layer

  • Xianfeng Dai,
  • Ke Xu and
  • Fanan Wei

Beilstein J. Nanotechnol. 2020, 11, 51–60, doi:10.3762/bjnano.11.5

Graphical Abstract
  • -step process owing to their improved crystal quality, crystal size, absorbance and stability. Furthermore, the perovskite films fabricated by the mesh-assisted grid technique are of controllable transparency and have shown the potential to be scalable. These attributes provide a promising route for the
PDF
Album
Review
Published 06 Jan 2020

The different ways to chitosan/hyaluronic acid nanoparticles: templated vs direct complexation. Influence of particle preparation on morphology, cell uptake and silencing efficiency

  • Arianna Gennari,
  • Julio M. Rios de la Rosa,
  • Erwin Hohn,
  • Maria Pelliccia,
  • Enrique Lallana,
  • Roberto Donno,
  • Annalisa Tirella and
  • Nicola Tirelli

Beilstein J. Nanotechnol. 2019, 10, 2594–2608, doi:10.3762/bjnano.10.250

Graphical Abstract
  • , cells were washed with PBS and incubated for 1 h at 37 °C in medium containing 5% (v/v) of MTS solution. Cell viability was measured by reading the absorbance values at 490 nm (Synergy2 Biotek plate reader using Gen5 software) and normalized against the total protein content in each well (BCA assay
  • ). Please note that any influence of phenol-red was ruled out by using medium as blank and subtracting its absorbance to all wells before calculating metabolic activity. Quantification of cell uptake. HCT-116 (20,000 cells/cm2) and RAW 264.7 (30,000 cells/cm2) were seeded in 12-well plates and left to
PDF
Album
Supp Info
Full Research Paper
Published 30 Dec 2019

Fully amino acid-based hydrogel as potential scaffold for cell culturing and drug delivery

  • Dávid Juriga,
  • Evelin Sipos,
  • Orsolya Hegedűs,
  • Gábor Varga,
  • Miklós Zrínyi,
  • Krisztina S. Nagy and
  • Angéla Jedlovszky-Hajdú

Beilstein J. Nanotechnol. 2019, 10, 2579–2593, doi:10.3762/bjnano.10.249

Graphical Abstract
  • proliferation reagent WST-1. The reagent was diluted (1:20) with αMEM containing no phenol red (Gibco, USA). Then, 200 μL solution was applied in each well. After incubation for 2 h at 37 °C, 150 μL of the supernatant solution was transferred from each well into an empty 96-well plate. The absorbance was
PDF
Album
Supp Info
Full Research Paper
Published 27 Dec 2019

pH-Controlled fluorescence switching in water-dispersed polymer brushes grafted to modified boron nitride nanotubes for cellular imaging

  • Saban Kalay,
  • Yurij Stetsyshyn,
  • Volodymyr Donchak,
  • Khrystyna Harhay,
  • Ostap Lishchynskyi,
  • Halyna Ohar,
  • Yuriy Panchenko,
  • Stanislav Voronov and
  • Mustafa Çulha

Beilstein J. Nanotechnol. 2019, 10, 2428–2439, doi:10.3762/bjnano.10.233

Graphical Abstract
  • the maximum fluorescein absorption is at 437 nm but in alkaline solution it is at about 490 nm, and the absorption intensity is significantly higher than that of the acidic solution. As seen in the figure, the highest intensity of the absorbance is at 490 nm for pH 10 (Figure 7, green line) and
  • slightly less at pH 7–8 (Figure 7, blue and red lines). In contrast, at pH 6 (Figure 7, yellow line) a strongly shifted, small, intense absorbance peak is observed. At pH 4 (Figure 7, green line) the absorbance is very poor, and we can state here that it has no absorbance. Only at pH 2 (Figure 7, brown
PDF
Album
Supp Info
Full Research Paper
Published 10 Dec 2019

Multiwalled carbon nanotube based aromatic volatile organic compound sensor: sensitivity enhancement through 1-hexadecanethiol functionalisation

  • Nadra Bohli,
  • Meryem Belkilani,
  • Juan Casanova-Chafer,
  • Eduard Llobet and
  • Adnane Abdelghani

Beilstein J. Nanotechnol. 2019, 10, 2364–2373, doi:10.3762/bjnano.10.227

Graphical Abstract
  • 1011), operating at 100 kV. An Alpha FTIR spectrometer (Bruker, France) equipped with an ATR platinum crystal diamond module was used, in absorbance mode, to obtain the infrared spectra of the gold-decorated MWCNTs before and after the deposition of the SAM monolayer. This technique provides useful
  • performance [25][26]. FTIR characterisation In order to ensure that the HDT monolayers are formed and well immobilized on the Au-MWCNT layers, we used FTIR spectroscopy. Figure 3 shows the infrared spectra, in absorbance mode, of both Au-MWCNT and HDT/Au-MWCNT layers. When analysing and comparing the infrared
PDF
Album
Supp Info
Full Research Paper
Published 04 Dec 2019

Mannosylated brush copolymers based on poly(ethylene glycol) and poly(ε-caprolactone) as multivalent lectin-binding nanomaterials

  • Stefania Ordanini,
  • Wanda Celentano,
  • Anna Bernardi and
  • Francesco Cellesi

Beilstein J. Nanotechnol. 2019, 10, 2192–2206, doi:10.3762/bjnano.10.212

Graphical Abstract
  • . In the case of the divalent compound PEG8-PCL2-Man2, the absorbance quickly reached a plateau and then remained almost constant, although a stepwise variation of the OD indicated a partial precipitation of the aggregates, as shown in Figure 6B. The same happened for the octavalent glycopolymer A4
PDF
Album
Supp Info
Full Research Paper
Published 07 Nov 2019

Incorporation of doxorubicin in different polymer nanoparticles and their anticancer activity

  • Sebastian Pieper,
  • Hannah Onafuye,
  • Dennis Mulac,
  • Jindrich Cinatl Jr.,
  • Mark N. Wass,
  • Martin Michaelis and
  • Klaus Langer

Beilstein J. Nanotechnol. 2019, 10, 2062–2072, doi:10.3762/bjnano.10.201

Graphical Abstract
  • ) sodium dodecylsulfate and 50% (v/v) N,N-dimethylformamide (pH 4.7) at 37 °C for 4 h. Absorbance was determined at 570 nm for each well using a 96-well multiscanner. After subtracting of the background absorption, the results are expressed as percentage viability relative to untreated control cultures
PDF
Album
Full Research Paper
Published 29 Oct 2019

Optimization and performance of nitrogen-doped carbon dots as a color conversion layer for white-LED applications

  • Tugrul Guner,
  • Hurriyet Yuce,
  • Didem Tascioglu,
  • Eren Simsek,
  • Umut Savaci,
  • Aziz Genc,
  • Servet Turan and
  • Mustafa M. Demir

Beilstein J. Nanotechnol. 2019, 10, 2004–2013, doi:10.3762/bjnano.10.197

Graphical Abstract
  • -Alpha XPS spectrometer. Fourier-transform infrared spectroscopy (FTIR; Spectrum 100, PerkinElmer, Shelton, CT, USA) was used to characterize the kinetic behavior of the chemical bonds. Photoluminescence (PL) and absorbance were measured using an integrating sphere (ISP-50-80-R, Ocean Optics Inc
PDF
Album
Supp Info
Full Research Paper
Published 15 Oct 2019

Nanostructured and oriented metal–organic framework films enabling extreme surface wetting properties

  • Andre Mähringer,
  • Julian M. Rotter and
  • Dana D. Medina

Beilstein J. Nanotechnol. 2019, 10, 1994–2003, doi:10.3762/bjnano.10.196

Graphical Abstract
  • of experiments establishes the pillar-like nanostructured Ni-CAT-1 films as highly efficient, antifog coatings featuring additional desired properties such as light absorbance. Furthermore, it underlines the power of VAC in enabling controlled bottom-up fabrication of MOF films with well-defined
PDF
Album
Supp Info
Full Research Paper
Published 09 Oct 2019

Gold-coated plant virus as computed tomography imaging contrast agent

  • Alaa A. A. Aljabali,
  • Mazhar S. Al Zoubi,
  • Khalid M. Al-Batanyeh,
  • Ali Al-Radaideh,
  • Mohammad A. Obeid,
  • Abeer Al Sharabi,
  • Walhan Alshaer,
  • Bayan AbuFares,
  • Tasnim Al-Zanati,
  • Murtaza M. Tambuwala,
  • Naveed Akbar and
  • David J. Evans

Beilstein J. Nanotechnol. 2019, 10, 1983–1993, doi:10.3762/bjnano.10.195

Graphical Abstract
  • increase in the absorbance shift is expected as the SPR of the AuNPs is sensitive to their interparticle distance and surface state [41]. Furthermore, the LSPR band of Au-CPMV is dependent on the size of the particles. The peak absorbance wavelength increases with the increase of the particle diameter as
  • together and incubated at 60 °C for 10 min. The samples were left to cool for 30 min and then centrifuged at 14000g for 40 min (Thermo Scientific CL10 Centrifuge) to pellet the particles. The supernatant BCA dye absorbance was measured at λ = 565 nm using a microplate reader. The change in absorbance is a
PDF
Album
Full Research Paper
Published 07 Oct 2019

Preservation of rutin nanosuspensions without the use of preservatives

  • Pascal L. Stahr and
  • Cornelia M. Keck

Beilstein J. Nanotechnol. 2019, 10, 1902–1913, doi:10.3762/bjnano.10.185

Graphical Abstract
  • time in the dark, the absorbance was measured by a UV–vis plate reader (Multiskan GO, Thermo scientific, Germany) at 517 nm. The inhibition activity (inhibition [%]) was calculated as where Asample is the absorbance of the sample and A0 is the absorbance of the control (DPPH solution). The resulting
PDF
Album
Full Research Paper
Published 19 Sep 2019

Engineered superparamagnetic iron oxide nanoparticles (SPIONs) for dual-modality imaging of intracranial glioblastoma via EGFRvIII targeting

  • Xianping Liu,
  • Chengjuan Du,
  • Haichun Li,
  • Ting Jiang,
  • Zimiao Luo,
  • Zhiqing Pang,
  • Daoying Geng and
  • Jun Zhang

Beilstein J. Nanotechnol. 2019, 10, 1860–1872, doi:10.3762/bjnano.10.181

Graphical Abstract
  • ) solution was added to each well. Finally, the absorbance of each well was measured with a microplate reader (Bio-TEK, USA) at a wavelength of 490 nm. To evaluate the in vivo systematic toxicity of PNPs, the histological sections of major organs (e.g., heart, liver, spleen, lung and kidney) were collected
PDF
Album
Full Research Paper
Published 11 Sep 2019

Microfluidic manufacturing of different niosomes nanoparticles for curcumin encapsulation: Physical characteristics, encapsulation efficacy, and drug release

  • Mohammad A. Obeid,
  • Ibrahim Khadra,
  • Abdullah Albaloushi,
  • Margaret Mullin,
  • Hanin Alyamani and
  • Valerie A. Ferro

Beilstein J. Nanotechnol. 2019, 10, 1826–1832, doi:10.3762/bjnano.10.177

Graphical Abstract
  • encapsulation efficiency Following removal of the non-encapsulated curcumin by dialysis, 100 µL of each niosome formulation (removed from the dialysis tubing) was lysed with methanol in order to release the encapsulated curcumin, which was then quantified at UV absorbance at 421 nm. The encapsulation efficiency
  • preheated at 37 °C. At each time point, the absorbance was measured at 421 nm and the concentration of the released curcumin was determined against a calibration curve. The experiments were performed in triplicate and the mean ± SD is reported. Statistical analysis Statistical significance was assessed by
PDF
Album
Full Research Paper
Published 05 Sep 2019

Toxicity and safety study of silver and gold nanoparticles functionalized with cysteine and glutathione

  • Barbara Pem,
  • Igor M. Pongrac,
  • Lea Ulm,
  • Ivan Pavičić,
  • Valerije Vrček,
  • Darija Domazet Jurašin,
  • Marija Ljubojević,
  • Adela Krivohlavek and
  • Ivana Vinković Vrček

Beilstein J. Nanotechnol. 2019, 10, 1802–1817, doi:10.3762/bjnano.10.175

Graphical Abstract
  • shaking the plates. The absorbance was recorded at 530 nm using a VictorTM multilabel reader (Perkin Elmer, Massachusetts, USA). The ratio of live, apoptotic and dead cells after treatment with NPs or respective metal salts was determined by flow cytometry experiments using Annexin V and 7
PDF
Album
Supp Info
Full Research Paper
Published 02 Sep 2019

Biocatalytic oligomerization-induced self-assembly of crystalline cellulose oligomers into nanoribbon networks assisted by organic solvents

  • Yuuki Hata,
  • Yuka Fukaya,
  • Toshiki Sawada,
  • Masahito Nishiura and
  • Takeshi Serizawa

Beilstein J. Nanotechnol. 2019, 10, 1778–1788, doi:10.3762/bjnano.10.173

Graphical Abstract
  • solution containing 10 vol % MeCN or EtOH at a concentration where the absorbance of CDP at 280 nm was 0.1. The CDP solutions were incubated at 60 °C for 6 and 72 h. The CD spectra of the samples were recorded on a J-725 instrument (JASCO) at a path length of 2 mm, a scan rate of 100 nm min−1, and a
PDF
Album
Correction
Full Research Paper
Published 26 Aug 2019

Growth dynamics and light scattering of gold nanoparticles in situ synthesized at high concentration in thin polymer films

  • Corentin Guyot,
  • Philippe Vandestrick,
  • Ingrid Marenne,
  • Olivier Deparis and
  • Michel Voué

Beilstein J. Nanotechnol. 2019, 10, 1768–1777, doi:10.3762/bjnano.10.172

Graphical Abstract
  • to aggregate yielding nanoparticles. These steps are enhanced by annealing the films at a temperature higher than the glass transition temperature of the polymer. In practice, the in situ synthesis allowed us to obtain relatively thin films (300–500 nm) with an absorbance equivalent to that measured
  • with a “classical” colloidal solution of AuNPs in a cuvette with 1 cm optical path (Figure 1). This means that the same absorbance can be obtained with a material thickness ca. 2·104 times smaller. Passive Q-switches based on saturable absorption of AuNP-doped films are usually prepared in a two-steps
  • mass ratio increases, the absorbance reaches an increasing plateau value. Moreover, increasing the annealing temperature increases the reduction kinetics. The plateau value is reached within shorter times. Besides playing a role in the growth kinetics of the nanoparticles by promoting the reduction of
PDF
Album
Supp Info
Full Research Paper
Published 23 Aug 2019

Doxorubicin-loaded human serum albumin nanoparticles overcome transporter-mediated drug resistance in drug-adapted cancer cells

  • Hannah Onafuye,
  • Sebastian Pieper,
  • Dennis Mulac,
  • Jindrich Cinatl Jr.,
  • Mark N. Wass,
  • Klaus Langer and
  • Martin Michaelis

Beilstein J. Nanotechnol. 2019, 10, 1707–1715, doi:10.3762/bjnano.10.166

Graphical Abstract
  • 20% (w/v) sodium dodecylsulfate and 50% (v/v) N,N-dimethylformamide with the pH adjusted to 4.7 at 37 °C for 4 h. The absorbance was determined at 570 nm for each well using a 96-well multiscanner. After subtracting of the background absorption, the results are expressed as percentage viability
PDF
Album
Supp Info
Full Research Paper
Published 14 Aug 2019
Other Beilstein-Institut Open Science Activities