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Search for "albumin" in Full Text gives 130 result(s) in Beilstein Journal of Nanotechnology.
Porous polymer coatings as substrates for the formation of high-fidelity micropatterns by quill-like pens
Beilstein J. Nanotechnol. 2013, 4, 377–384, doi:10.3762/bjnano.4.44
- micrometres. The bromophenol blue arrays on HEMA support were used to detect the presence of bovine serum albumin (BSA). In the presence of BSA, the fluorescence spectrum observed from the bromophenol blue microarray exhibited a significant red shift of the maximum emission wavelength. Our results show that
- samples [12][13]. After preparing 10 × 10 spot microarrays using bromophenol blue solution as shown in the inset of Figure 6b, the patterns were either incubated with a 0.5 µL droplet of an analyte solution containing bovine serum albumin (BSA) or a clear solution to give a negative control. Fluorescence
Macromolecular shape and interactions in layer-by-layer assemblies within cylindrical nanopores
Beilstein J. Nanotechnol. 2012, 3, 475–484, doi:10.3762/bjnano.3.54
- serum albumin was compared with that of linear polyelectrolytes (linear-PEs), both species being of similar molecular weight. LbL deposition within the cylindrical AAO geometry for different pore diameters (d0 = 25–80 nm) for the various macromolecular species, showed that the multilayer film growth was
- molecular recognition of biotinylated-bovine serum albumin (b-BSA) by avidin. Avidin has four biotin-binding sites, whereas the b-BSA used has 13 biotin molecules per protein on average. Avidin with a mass of MW = 66–69 kDa, and which is positively charged at neutral pH, was first adsorbed onto the
- Calbiochem (purity 12.9 units/mg). Biotinylated-bovine serum albumin (b-BSA) with 13 mol biotin/mol albumin, poly(sodium 4-styrenesulfonate) (PSS) (Mw = 70 kDa), poly(allylamine hydrochloride) PAH (Mw = 50–65 kDa), CuCl2, NaCl, and 16-mercaptohexadecanoic acid (90%) were purchased from Sigma Aldrich (St
Analysis of fluid flow around a beating artificial cilium
Beilstein J. Nanotechnol. 2012, 3, 163–171, doi:10.3762/bjnano.3.16
- diameter 55 nm [24]) in water. To prevent aggregation of the beads, we coated them with BSA (bovine serum albumin), 10 mg/mL, for 4 h in an ultrasonic bath. One end of the assembled chain was attached to the surface through prefabricated ferromagnetic-nickel anchoring sites. The nickel dots were
Characterization of protein adsorption onto FePt nanoparticles using dual-focus fluorescence correlation spectroscopy
Beilstein J. Nanotechnol. 2011, 2, 374–383, doi:10.3762/bjnano.2.43
- , namely serum albumin, apolipoprotein A-I and apolipoprotein E4, onto polymer-coated, fluorescently labeled FePt nanoparticles (~12 nm diameter) carrying negatively charged carboxyl groups on their surface. For all three proteins, a step-wise increase in hydrodynamic radius with protein concentration was
- correlation spectroscopy; human serum albumin; nanoparticle; protein adsorption; Introduction Recent years have seen enormous advances in the field of nanotechnology. A huge variety of nanoparticles (NPs), defined as objects with all three spatial dimensions in the range of 1–100 nm, has been developed, with
- understand the structural and dynamic properties of the protein corona at the molecular level. Recently, we have used quantitative fluorescence microscopy, especially fluorescence correlation spectroscopy (FCS), to study protein adsorption of human serum albumin (HSA) on polymer-coated FePt NPs with an
Magnetic nanoparticles for biomedical NMR-based diagnostics
Beilstein J. Nanotechnol. 2010, 1, 142–154, doi:10.3762/bjnano.1.17
- bovine serum albumin (BSA) protein as a control did not elicit any change in T2 (Figure 5b). More recently, MRSw biosensors, capable of detecting soluble tumor biomarker proteins (such as CA-125, VEGF, and α-fetoprotein) were described, and used for parallel detection of multiple markers in blood samples
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