Search results

Search for "pH" in Full Text gives 646 result(s) in Beilstein Journal of Nanotechnology. Showing first 200.

A nonenzymatic reduced graphene oxide-based nanosensor for parathion

  • Sarani Sen,
  • Anurag Roy,
  • Ambarish Sanyal and
  • Parukuttyamma Sujatha Devi

Beilstein J. Nanotechnol. 2022, 13, 730–744, doi:10.3762/bjnano.13.65

Graphical Abstract
  • monitoring of PT. For this purpose, the electrochemical reduction of GO was tuned using various electrolytic buffers with different pH values, supporting the variation of the physicochemical characteristics of ERGO discussed in this work. Besides, this study highlights the scope of an interference-free
  • nonenzymatic approach through electrochemical nanosensing which can also be used in other biosensing applications. Experimental Chemicals The chemicals used in this work are summarized in Supporting Information File 1, Table S1. Sodium dihydrogen phosphate was used to prepare phosphate buffer saline (pH 4.6
  • , 7.4, 9). Acetate buffer (20 mM, pH 4.5), and Britton–Robinson (BR) buffer (40 mM, pH 4) consisting of phosphoric acid, boric acid, and acetic acid were also prepared. Synthesis of graphene oxide Graphene oxide was synthesized from graphite powder using a modified Hummer’s method [30][31]. In detail
PDF
Album
Supp Info
Full Research Paper
Published 28 Jul 2022

Design and selection of peptides to block the SARS-CoV-2 receptor binding domain by molecular docking

  • Kendra Ramirez-Acosta,
  • Ivan A. Rosales-Fuerte,
  • J. Eduardo Perez-Sanchez,
  • Alfredo Nuñez-Rivera,
  • Josue Juarez and
  • Ruben D. Cadena-Nava

Beilstein J. Nanotechnol. 2022, 13, 699–711, doi:10.3762/bjnano.13.62

Graphical Abstract
  • , peptides with great affinity to the RBD were selected. The most common amino acids involved in the recognition of the RBD were identified to design novel peptides based on the number of hydrogen bonds that were formed. At physiological pH, these peptides are almost neutral and soluble in aqueous media
  • temporin B and lysozyme (61–80). The net charge calculated for the OAPs varies according to the number of negatively and positively charged amino acids present in the primary structure. The net charge values are in the range of −3.9 to 2.9 at pH 7. The OAPs were selected based on an absolute value of the
  • electrical net charge smaller than 3.9 (|charge| ≤ 3.9, which is the net charge of ACE2), to avoid possible cytotoxic effects [54][55]. Given that, at physiological pH, the RBD active region is positively charged, it could be assumed that negatively charged peptides, such as sesquin and MVL (74–87) (Figure
PDF
Album
Supp Info
Full Research Paper
Published 22 Jul 2022

Sodium doping in brookite TiO2 enhances its photocatalytic activity

  • Boxiang Zhuang,
  • Honglong Shi,
  • Honglei Zhang and
  • Zeqian Zhang

Beilstein J. Nanotechnol. 2022, 13, 599–609, doi:10.3762/bjnano.13.52

Graphical Abstract
  • . Then 1.5 mL of TBOT was slowly dropped into the solution to form a transparent yellow sol by vigorously stirring for a full reaction between TBOT and oxalic acid. The pH value of the sol was adjusted to 10 by using 2 mol/L of NaOH solution. After stirring for another 1 h, the sol was transferred into a
  • 100 mL Teflon-lined autoclave and heated at 180 °C for 8 h. The white precipitate was collected by centrifugation (8000 rpm, 10 min), washed to a neutral pH with distilled water and absolute ethanol, and then dried at 80 °C. To inspect the effect of the calcinating temperature on the structure of the
PDF
Album
Supp Info
Full Research Paper
Published 05 Jul 2022

A new method for obtaining the magnetic shape anisotropy directly from electron tomography images

  • Cristian Radu,
  • Ioana D. Vlaicu and
  • Andrei C. Kuncser

Beilstein J. Nanotechnol. 2022, 13, 590–598, doi:10.3762/bjnano.13.51

Graphical Abstract
  • nanoparticle system. Experimental For the preparation of the magnetite MNPs, the following starting materials were used as purchased: FeSO4·7H2O (Merck, 99.5%), hexadecyltrimethylammonium bromide (CTAB, 99+%, Acros Organics), NaOH (puriss p.a., ACS reagent, reag. Ph. Eur., K < 0.02%, ≥98%, pellets), NH4OH (for
PDF
Album
Supp Info
Full Research Paper
Published 05 Jul 2022

Detection and imaging of Hg(II) in vivo using glutathione-functionalized gold nanoparticles

  • Gufeng Li,
  • Shaoqing Li,
  • Rui Wang,
  • Min Yang,
  • Lizhu Zhang,
  • Yanli Zhang,
  • Wenrong Yang and
  • Hongbin Wang

Beilstein J. Nanotechnol. 2022, 13, 549–559, doi:10.3762/bjnano.13.46

Graphical Abstract
  • mentioning here that the pH value of the reaction systems played an important role in the synthesis of GSH-Rh6G2. Rh6G2, as an ideal candidate for controlled-release molecular systems, shows little fluorescence (Figure 1b). In the absence of GNPs, the conjugation of GSH-Rh6G2 yields obvious fluorescence. The
  • S1, Supporting Information File 1) [42]. The final product of the reaction between Rh6G2 and GSH was proved by TOF-MS analysis (Figure S2, Supporting Information File 1). By adjusting the pH value from an unadjusted slightly acidic environment to pH 7, a significant decrease in the fluorescence of
  • first six hours as shown in Figure 4a. Furthermore, the pH value is critical regarding the fluorescence intensity. GNPs-GSH-Rh6G2 displayed a large change in the fluorescence intensity in the pH range from 1 to 5 (Figure 4b). This indicates that Rh6G2 tends to be protonated at low pH values, which
PDF
Album
Supp Info
Full Research Paper
Published 23 Jun 2022

Stimuli-responsive polypeptide nanogels for trypsin inhibition

  • Petr Šálek,
  • Jana Dvořáková,
  • Sviatoslav Hladysh,
  • Diana Oleshchuk,
  • Ewa Pavlova,
  • Jan Kučka and
  • Vladimír Proks

Beilstein J. Nanotechnol. 2022, 13, 538–548, doi:10.3762/bjnano.13.45

Graphical Abstract
  • biocompatible Nα-ʟ-lysine-grafted α,β-poly[(2-propyne)-ᴅ,ʟ-aspartamide-ran-(2-hydroxyethyl)-ᴅʟ-aspartamide-ran-(2-(4-hydroxyphenyl)ethyl)-ᴅʟ-aspartamide] (Nα-Lys-NG). Both nanogels were prepared by HRP/H2O2-mediated crosslinking in inverse miniemulsions with pH and temperature-stimuli responsive behavior
  • acid-labile cholesteryl-modified pullulan nanogel that formed a complex with loaded bovine serum albumin and released it under acidic conditions [17]. Landfester et al. used a bio-orthogonal reaction to fabricate a dextran nanogel with pH-responsive hydrazine linkages allowing for a controlled release
  • biodegradable PHEG-Tyr and biocompatible and non-biodegradable Nα-Lys-NG polypeptide precursors by HRP/H2O2-mediated crosslinking in inverse miniemulsion for the delivery of AAT. Both prepared polypeptide nanogels demonstrate stimuli-responsive behavior under different pH values and temperatures. After
PDF
Album
Full Research Paper
Published 22 Jun 2022

Influence of thickness and morphology of MoS2 on the performance of counter electrodes in dye-sensitized solar cells

  • Lam Thuy Thi Mai,
  • Hai Viet Le,
  • Ngan Kim Thi Nguyen,
  • Van La Tran Pham,
  • Thu Anh Thi Nguyen,
  • Nguyen Thanh Le Huynh and
  • Hoang Thai Nguyen

Beilstein J. Nanotechnol. 2022, 13, 528–537, doi:10.3762/bjnano.13.44

Graphical Abstract
  • exhibited two redox couple peaks at −0.34 V/−0.76 V and −0.77 V/−1.34 V attributed to the redox reactions of Mo7O246− and MoO42− ions, respectively [27]. The presence of MoO42− ions is due to the equilibrium in Equation 3, which occurs in acidic solution of (NH4)6Mo7O24 (the pH here is about 4.3). The CV
  • ) and Na2S in acidic media (adjusted to pH 6) is very favorable to the preparation of MoS42− precursor solution. In this work, the optimal concentration ratio of (NH4)2Mo7O24 (mM) to Na2S (g/L) was found to be 1:6 (data not shown). It can be seen from the CV curve of the mixture solution that the
  • )6Mo7O24 (x mM) and Na2S (y g·L−1) in distilled water, the pH of the solution was adjusted to 6.0 using a 20% (v/v) H2SO4 solution, KCl (0.1 M) was used as the supporting electrolyte. The concentration ratio between (NH4)2Mo7O24 and Na2S was kept constant (x/y = 1:6) with different concentration levels
PDF
Album
Supp Info
Full Research Paper
Published 17 Jun 2022

Ciprofloxacin-loaded dissolving polymeric microneedles as a potential therapeutic for the treatment of S. aureus skin infections

  • Sharif Abdelghany,
  • Walhan Alshaer,
  • Yazan Al Thaher,
  • Maram Al Fawares,
  • Amal G. Al-Bakri,
  • Saja Zuriekat and
  • Randa SH. Mansour

Beilstein J. Nanotechnol. 2022, 13, 517–527, doi:10.3762/bjnano.13.43

Graphical Abstract
  • isocratic composition of 0.025 M phosphoric acid (pH 3.0 ± 0.1) previously adjusted with triethylamine/acetonitrile (80:20 v/v), eluted at a flow rate of 1.5 mL/min. The injection volume was 20 µL. The drug concentration was then determined according to a calibration curve. The calibration curve was plotted
  • × phosphate-buffered saline (PBS), pH 7.4, for 15 min. A section of full-thickness human skin was placed, with the stratum corneum facing upward, onto a piece of tissue paper soaked with PBS in a weighing boat. CIP_MN1 were adhered to a piece of plastic tape and manually applied to the skin. To prevent the
PDF
Album
Full Research Paper
Published 15 Jun 2022

Design and characterization of polymeric microneedles containing extracts of Brazilian green propolis

  • Camila Felix Vecchi,
  • Rafaela Said dos Santos,
  • Jéssica Bassi da Silva and
  • Marcos Luciano Bruschi

Beilstein J. Nanotechnol. 2022, 13, 503–516, doi:10.3762/bjnano.13.42

Graphical Abstract
  • final dispersions were filtered through grade-3 filter paper [44][45]. The pH determination was performed using a pHmeter calibrated with buffer solutions. The relative density of the extract was determined using a pycnometer calibrated at 20 °C in a controlled temperature environment (20 °C). To
PDF
Album
Supp Info
Full Research Paper
Published 08 Jun 2022

Ethosomal (−)-epigallocatechin-3-gallate as a novel approach to enhance antioxidant, anti-collagenase and anti-elastase effects

  • Çiğdem Yücel,
  • Gökçe Şeker Karatoprak,
  • Sena Yalçıntaş and
  • Tuğba Eren Böncü

Beilstein J. Nanotechnol. 2022, 13, 491–502, doi:10.3762/bjnano.13.41

Graphical Abstract
  • regarding organoleptic properties, pH values, and viscosity. Stability studies were conducted for three months and changes in characterization parameters and residual EGCG content of ETHs were examined. Besides, for ETHG, organoleptic properties, pH values (every two weeks), and viscosity (first and twelfth
  • obtained in the F3-coded ETH. Afterward, the ethosomal gel formulation was prepared by dispersing the F3 formulation in 1% (w/v) Carbopol 980 gel. Next, its organoleptic properties (i.e., color and homogeneity), viscosity and rheological properties, and pH values were evaluated. It was found suitable to
  • use Carbopol 980 as the gelling agent at a ratio of 1:1 (v/v) due to its suitable organoleptic properties, pH value, viscosity, and its ability to homogeneously mix with the ethosome. A transparent, homogeneous, and smooth ethosomal gel system with a skin-compatible pH value (5.60) was obtained (Table
PDF
Album
Full Research Paper
Published 31 May 2022

Zinc oxide nanostructures for fluorescence and Raman signal enhancement: a review

  • Ioana Marica,
  • Fran Nekvapil,
  • Maria Ștefan,
  • Cosmin Farcău and
  • Alexandra Falamaș

Beilstein J. Nanotechnol. 2022, 13, 472–490, doi:10.3762/bjnano.13.40

Graphical Abstract
  • nanotetrapods [26]. Size and shape of the ZnO NPs depend on the type of precursor salts used (e.g., chlorides, sulfates, nitrates, or perchlorates), the molar ratio between reagents, the reaction temperature, the pH value, the ionic concentration of the medium, and other reaction parameters. The main advantage
PDF
Album
Review
Published 27 May 2022

Tunable superconducting neurons for networks based on radial basis functions

  • Andrey E. Schegolev,
  • Nikolay V. Klenov,
  • Sergey V. Bakurskiy,
  • Igor I. Soloviev,
  • Mikhail Yu. Kupriyanov,
  • Maxim V. Tereshonok and
  • Anatoli S. Sidorenko

Beilstein J. Nanotechnol. 2022, 13, 444–454, doi:10.3762/bjnano.13.37

Graphical Abstract
  • = 1 μm, and total thickness d = 80 nm (this corresponds to the spacer thickness ds =5 nm) the estimated kinetic inductance is about 7 pH in the closed state and about 15 pH in the open state. For comparison, the geometric inductance of such a strip is of the order of 1 pH. Conclusion We have
PDF
Album
Full Research Paper
Published 18 May 2022

A non-enzymatic electrochemical hydrogen peroxide sensor based on copper oxide nanostructures

  • Irena Mihailova,
  • Vjaceslavs Gerbreders,
  • Marina Krasovska,
  • Eriks Sledevskis,
  • Valdis Mizers,
  • Andrejs Bulanovs and
  • Andrejs Ogurcovs

Beilstein J. Nanotechnol. 2022, 13, 424–436, doi:10.3762/bjnano.13.35

Graphical Abstract
  • = 0 V vs Ag/AgCl and a scan rate of 100 mV/s. As buffer solution, 0.1 M NaOH (pH 12.7) was used. For the determination of H2O2, 0.1, 0.25, 0.5, 0.65, 0.85, 1, and 5 mM concentrations were used. Measurements were carried out five times for each of the indicated concentrations, and the curves in the
  • following sections show the averaged data from all measurements. To determine the optimal scanning parameters that provide the maximum sensitivity of the sensor, the dependence of the electrochemical response on the pH of the buffer solution and on the scanning speed was studied. Impedance spectroscopy was
  • pH of the buffer solution and on the pulse frequency was studied. Current response study For the current response study (i–t measurement), a constant voltage U = −0.7 V vs Ag/AgCl was applied to the cell and the current was measured. 0.1 M NaOH was used as buffer solution. The measurement was started
PDF
Album
Full Research Paper
Published 03 May 2022

Alcohol-perturbed self-assembly of the tobacco mosaic virus coat protein

  • Ismael Abu-Baker and
  • Amy Szuchmacher Blum

Beilstein J. Nanotechnol. 2022, 13, 355–362, doi:10.3762/bjnano.13.30

Graphical Abstract
  • and prevent the formation of helical rods at low pH. A high alcohol content favours stacked disk assemblies and large rafts, while a low alcohol concentration favours individual disks and short stacks. These effects appear to be caused by the hydrophobicity of the alcohol additive, with isopropyl
  • range of different structures depending mainly on pH and ionic strength (Figure 1). Above neutral pH and at low to moderate ionic strength, the protein exists primarily as a mixture of monomers and small oligomers. This mixture is known as A-protein [17]. Around pH 6.5–7.0, TMV-cp assembles into achiral
  • bilayer disks composed of 17 monomers per layer, 18 nm in diameter with a 4 nm central channel. At high ionic strength and non-acidic pH, these disks can stack on top of each other to form non-helical, rod-like assemblies. There are several known disk aggregates that can be difficult to distinguish
PDF
Album
Supp Info
Full Research Paper
Published 01 Apr 2022

A broadband detector based on series YBCO grain boundary Josephson junctions

  • Egor I. Glushkov,
  • Alexander V. Chiginev,
  • Leonid S. Kuzmin and
  • Leonid S. Revin

Beilstein J. Nanotechnol. 2022, 13, 325–333, doi:10.3762/bjnano.13.27

Graphical Abstract
  • (Z) = RL, dIm(Z)/dω = 2LL, and . For the serial resonance at 250 GHz, RL = 43 Ω, LL = 500 pH, and CL = 0.8 fF. Figure 5, right, shows the current–voltage characteristics (IVCs) of a single junction with the antenna under the influence of an external signal. A bias current regime with the optimum
PDF
Album
Full Research Paper
Published 28 Mar 2022

The effect of metal surface nanomorphology on the output performance of a TENG

  • Yiru Wang,
  • Xin Zhao,
  • Yang Liu and
  • Wenjun Zhou

Beilstein J. Nanotechnol. 2022, 13, 298–312, doi:10.3762/bjnano.13.25

Graphical Abstract
  • of a metal and a polymer. There are different charge densities on different kinds of metal surface nanomorphology, which significantly influences the output performance of the TENG. Copper samples with different nanomorphology were obtained by controlling pH value, current density, electrolyte
  • freely. Therefore, there is no high charge density on the tip surface of the polymer surface. Here, size and morphology of nanoscale copper were controlled by adjusting current density, temperature, pH value, and solution concentration during electrodeposition. The effects of different morphologies and
  • and copper oxide was activated at the same time. After acid oil removal, the copper sheets were washed with deionized water at 25 and 50 °C, and the residuals from the previous process were cleaned to avoid polluting the electroplating solution. Different pH values were set using 1 M sulfuric acid
PDF
Album
Full Research Paper
Published 15 Mar 2022

Coordination-assembled myricetin nanoarchitectonics for sustainably scavenging free radicals

  • Xiaoyan Ma,
  • Haoning Gong,
  • Kenji Ogino,
  • Xuehai Yan and
  • Ruirui Xing

Beilstein J. Nanotechnol. 2022, 13, 284–291, doi:10.3762/bjnano.13.23

Graphical Abstract
  • , and allergens [17]. In spite of the tremendous potential, Myr possesses the same shortcomings as many hydrophobic small molecules, namely low bioavailability, poor water solubility and rapid degradation at pH > 6.8, which limits its clinical therapeutic potential [22]. GSH consists of glycine
  • quantitative stoichiometry analysis. The quantitative component analysis by UV–vis and ICP-OES proved that the molar ratio of Myr/GSH/Zn2+ was close to 1:1:1 (Supporting Information File 1, Table S1 and Figure S1). The UV–vis absorption spectra of pure Myr dispersed in 0.1 M NaOH (pH 13), Myr/Zn2+ complex (pH
  • 5.5), and MZG nanoparticles (pH 5.5) were measured. The UV–vis absorption spectrum of MZG nanoparticles exhibited a blue shift at 550 nm, compared with Myr/Zn2+, which was assigned to the charge transfer between GSH and Zn2+ (Figure 2c). These results demonstrated that Zn2+ as the coordination
PDF
Album
Supp Info
Correction
Full Research Paper
Published 01 Mar 2022

Systematic studies into uniform synthetic protein nanoparticles

  • Nahal Habibi,
  • Ava Mauser,
  • Jeffery E. Raymond and
  • Joerg Lahann

Beilstein J. Nanotechnol. 2022, 13, 274–283, doi:10.3762/bjnano.13.22

Graphical Abstract
  • water and ethanol with a 9:1 (v/v) ratio. The exception was insulin and mucin-based SPNPs. Because insulin has poor solubility at neutral pH, acetic acid at 10% (v/v) was added to the solvent mixture to ensure miscibility. Due to its high molecular weight, mucin was used at 2% (w/v). NHS-PEG-NHS
PDF
Album
Supp Info
Full Research Paper
Published 28 Feb 2022

Effects of drug concentration and PLGA addition on the properties of electrospun ampicillin trihydrate-loaded PLA nanofibers

  • Tuğba Eren Böncü and
  • Nurten Ozdemir

Beilstein J. Nanotechnol. 2022, 13, 245–254, doi:10.3762/bjnano.13.19

Graphical Abstract
  • under nitrogen atmosphere. Encapsulation efficiency of electrospun nanofibers Nanofibers (1 × 1 cm) were weighed and 1 mL of dichloromethane was added to dissolve fiber mats and 1 h later 7 mL of phosphate buffer solution (pH 7.4) was added to dissolve the drug released from the nanofibers (n = 3
  • . Encapsulation efficiency = (the amount of drug loaded/theoretical drug amount in the nanofiber) × 100. In vitro drug release The static method was used to evaluate the in vitro drug release of the nanofiber mats. Nanofiber mats (2 × 2 cm) were weighed and incubated in 5 mL of phosphate buffer solution (pH 7.4
PDF
Album
Full Research Paper
Published 21 Feb 2022

Relationship between corrosion and nanoscale friction on a metallic glass

  • Haoran Ma and
  • Roland Bennewitz

Beilstein J. Nanotechnol. 2022, 13, 236–244, doi:10.3762/bjnano.13.18

Graphical Abstract
  • -spinning technique and provided by the Physics Institute at the University of Basel (Switzerland). The X-ray diffraction of Cu Kα radiation (XRD) verified the amorphous nature of the ribbons. All friction experiments were conducted at room temperature in 0.2 M phosphate buffer (Na2HPO4 + NaH2PO4, pH ≈ 7
PDF
Album
Supp Info
Full Research Paper
Published 18 Feb 2022

Engineered titania nanomaterials in advanced clinical applications

  • Padmavati Sahare,
  • Paulina Govea Alvarez,
  • Juan Manual Sanchez Yanez,
  • Gabriel Luna-Bárcenas,
  • Samik Chakraborty,
  • Sujay Paul and
  • Miriam Estevez

Beilstein J. Nanotechnol. 2022, 13, 201–218, doi:10.3762/bjnano.13.15

Graphical Abstract
  • of hydroxy groups elicits hydrophobicity [24]. The primary physical properties of titanium dioxide that contribute to its biocompatibility are high corrosion resistance, the thermodynamic state at low physiological pH values, the isoelectric point of 5–6, the low ion formation tendency in aqueous
  • environments, and a high strength-to-weight ratio. Moreover, titanium is somewhat negatively charged at physiological pH values because of the formation of a passive oxide layer, and its dielectric constant is equivalent to that of water [25]. The specific energy structure of TiO2 is responsible for its
  • hindrance inside the tubular structure. This stage of drug release is known as sustained release. The controlled release of drugs is triggered by various external or internal stimuli. Changes in pH value, redox reactions, and enzyme activity are internal stimuli, while light, magnetic fields, and ultrasound
PDF
Album
Review
Published 14 Feb 2022

A comprehensive review on electrospun nanohybrid membranes for wastewater treatment

  • Senuri Kumarage,
  • Imalka Munaweera and
  • Nilwala Kottegoda

Beilstein J. Nanotechnol. 2022, 13, 137–159, doi:10.3762/bjnano.13.10

Graphical Abstract
  • mixture of PVA, sodium alginate, and PEO with incorporated nano-sized ZSM5 zeolite, which contains hydroxy functional groups (HZSM5). The maximum adsorption values of Th4+ and U6+ ions were found to be 274.6 and 144.7 mg/g, respectively, at a HZSM5 content of 10 wt %, an adsorbent dosage of 1 g/L, and pH
  • fused SiO2 NPs. The prepared nanohybrid showed efficient separation of oil and water with excellent stability in the range of pH 2–12, indicating its potential to be used in oil spill cleanup and the treatment of industrial oil-polluted water [70]. Ma and co-workers also fabricated an oil–water
  • work, a mildly hydrolyzed PAN membrane has been modified by fusing bovine serum albumin (BSA) obtained from laboratory wastes, and it has shown a maximum dye removal capacity of 434.78 mg/g, at pH 12 for TBO. The membrane has the ability to be regenerated by eluting the dye completely with 1 M NaCl or
PDF
Album
Review
Published 31 Jan 2022

Bacterial safety study of the production process of hemoglobin-based oxygen carriers

  • Axel Steffen,
  • Yu Xiong,
  • Radostina Georgieva,
  • Ulrich Kalus and
  • Hans Bäumler

Beilstein J. Nanotechnol. 2022, 13, 114–126, doi:10.3762/bjnano.13.8

Graphical Abstract
  • the CCD method after precipitation, as well as after cross-linking, dissolution, and final washing steps are shown in Figure 1. The zeta potential of HbMP in phosphate-buffered saline (PBS), pH 7.4, was −8.51 ± 0.9 mV. The zeta potential in PBS, pH 7.0, of E. coli was −16 mV, that of S. epidermidis
  • by centrifugation and washed three times with 0.9% NaCl. The particles were resuspended in a 0.02% GA solution and incubated for 1 h at room temperature on a shaker (cross-linking). After another centrifugation, the excess GA was quenched with 0.1 M of glycine. A solution of 0.2 M of EDTA pH 7.4 was
  • utilizing an excitation wavelength of 488 nm and a 505 nm long-pass emission filter. The zeta potential of HbMP in 0.9% NaCl (pH 7.4, conductivity 17.2 ± 0.9 mS/cm) was measured using the Zetasizer Nano ZS instrument. For the determination of the concentration of free hemoglobin in the HbMP suspension
PDF
Album
Full Research Paper
Published 24 Jan 2022

Theranostic potential of self-luminescent branched polyethyleneimine-coated superparamagnetic iron oxide nanoparticles

  • Rouhollah Khodadust,
  • Ozlem Unal and
  • Havva Yagci Acar

Beilstein J. Nanotechnol. 2022, 13, 82–95, doi:10.3762/bjnano.13.6

Graphical Abstract
  • 80 °C under argon atmosphere. The black solution was cooled to room temperature after 30 min and acidified to pH 5 with CH3COOH (Lachema, Czech Republic). The final product SPION@bPEI was washed with DI water using 30 kDa Amicon centrifugal filters and stored at room temperature. The total organic
  • -hydroxysulfosuccinimide (sulfo-NHS) (Sigma-Aldrich, USA) in 2-(N-morpholino)ethanesulfonic acid (MES) buffer (Biomatik, Canada) at pH 6.0 at room temperature for 15 min. Then, NHS-activated Erb (152 kDa) was washed with PBS (Biomatik, Canada) using an ultracentrifugation filter (10 kDa MWCO Amicon) and then added to 120
  • quantified by the absorbance at 260 nm using the Nanodrop. Different volumes of PIC or plasmid (500 µg/mL) and SPION@bPEI (5 mg/mL) in HEPES-buffered glucose (HBG, 20 mM of HEPES, 5% w/v of glucose) pH 7.4 were mixed at a final volume of 250 µL and incubated at room temperature for 30 min to generate a
PDF
Album
Supp Info
Full Research Paper
Published 18 Jan 2022

Biocompatibility and cytotoxicity in vitro of surface-functionalized drug-loaded spinel ferrite nanoparticles

  • Sadaf Mushtaq,
  • Khuram Shahzad,
  • Tariq Saeed,
  • Anwar Ul-Hamid,
  • Bilal Haider Abbasi,
  • Nafees Ahmad,
  • Waqas Khalid,
  • Muhammad Atif,
  • Zulqurnain Ali and
  • Rashda Abbasi

Beilstein J. Nanotechnol. 2021, 12, 1339–1364, doi:10.3762/bjnano.12.99

Graphical Abstract
  • specificity with significantly higher (p < 0.005) drug release in an acidic environment (pH 5.5). The nanoparticles were highly colloidal (zeta potential = −35 to −26 mV) in deionized water, phosphate buffer saline (PBS), and sodium borate buffer (SBB). They showed elevated and dose-dependent cytotoxicity in
  • ) dispersed in deionized water, sodium borate buffer (SBB) pH 9.0, phosphate buffer saline (PBS) pH 7.4, and DMEM were used for zeta potential measurements. The purpose of using buffers (i.e., SBB, PBS) was to get indirect surface charge information. Deionized water was used to check the influence of
  • applications [30]. All samples have lower PDI values (0.13–0.33) which indicates a uniform distribution of NPs in different dispersion media (Table 5). pH-dependent drug-loading and drug-release kinetics The UV–vis-based confirmation of drug (DOX and MTX) attachment to PMA-coated MFe2O4 (M = Fe, Co, Zn, Ni
PDF
Album
Full Research Paper
Published 02 Dec 2021
Other Beilstein-Institut Open Science Activities