Phospholipid arrays on porous polymer coatings generated by micro-contact spotting

• Sylwia Sekula-Neuner,
• Monica de Freitas,
• Lea-Marie Tröster,
• Tobias Jochum,
• Pavel A. Levkin,
• Michael Hirtz and
• Harald Fuchs

Beilstein J. Nanotechnol. 2017, 8, 715–722, doi:10.3762/bjnano.8.75

• microscopy and quantification of signal Fluorescence microscopy was carried out on an Eclipse 80i upright microscope (Nikon Instruments Inc.). Fluorescence images were taken with 10× or 20× objectives. Images were recorded with a CoolSNAP HQ2 camera (Photometrics). Patterns were aligned for imaging by using

The longstanding challenge of the nanocrystallization of 1,3,5-trinitroperhydro-1,3,5-triazine (RDX)

• Florent Pessina and
• Denis Spitzer

Beilstein J. Nanotechnol. 2017, 8, 452–466, doi:10.3762/bjnano.8.49

• quantification by Rietveld or full pattern matching methods would have been useful to follow the conversion with time. Dry production methods Physical vapor deposition (PVD) In 2002, Frolov and Pivkina first reported on a vacuum condensation process for high energetic materials [38][39][40]. The vacuum

Study of the surface properties of ZnO nanocolumns used for thin-film solar cells

• Neda Neykova,
• Jiri Stuchlik,
• Karel Hruska,
• Ales Poruba,
• Zdenek Remes and
• Ognen Pop-Georgievski

Beilstein J. Nanotechnol. 2017, 8, 446–451, doi:10.3762/bjnano.8.48

• profiles. The analyzer transmission function, Scofield sensitivity factors, and effective attenuation length for photoelectrons were applied for quantification. All spectra were referenced to the adventitious C 1s peak at a binding energy (BE) of 285.0 eV. The BE scale was controlled on standards of poly

Uptake of the proteins HTRA1 and HTRA2 by cells mediated by calcium phosphate nanoparticles

• Olga Rotan,
• Katharina N. Severin,
• Simon Pöpsel,
• Alexander Peetsch,
• Melisa Merdanovic,
• Michael Ehrmann and
• Matthias Epple

Beilstein J. Nanotechnol. 2017, 8, 381–393, doi:10.3762/bjnano.8.40

• dispersion in biological media containing proteins (like RPMI/10% FCS) is much better due to the formation of a protein corona [48][49]. The concentration of the labelled proteins was determined by UV–vis spectroscopy (Table 2). Table 3 gives the quantification data of the functionalized nanoparticles, where

Surface-enhanced Raman scattering of self-assembled thiol monolayers and supported lipid membranes on thin anodic porous alumina

• Marco Salerno,
• Amirreza Shayganpour,
• Barbara Salis and
• Silvia Dante

Beilstein J. Nanotechnol. 2017, 8, 74–81, doi:10.3762/bjnano.8.8

• quantification of the adsorbed mass onto the surface of a vibrating Au-coated quartz electrode through the measurement of the mass-induced frequency shift. Additionally, the measurement of the dissipation gives indication about the viscoelastic properties of the adsorbed layer. The quartz–Au substrate was thus

The cleaner, the greener? Product sustainability assessment of the biomimetic façade paint Lotusan^® in comparison to the conventional façade paint Jumbosil^®

• Florian Antony,
• Rainer Grießhammer,
• Thomas Speck and
• Olga Speck

Beilstein J. Nanotechnol. 2016, 7, 2100–2115, doi:10.3762/bjnano.7.200

• utility. In order to be able to finally assess which product, over the entire observation period and with regard to all relevant aspects of utility, performs as the better product, a systematic and integrated evaluation is required. A robust quantification of the functional utility of the two compared

• or, in other terms, an arbitrary shortening of the service life time. On the other hand the efforts of a façade cleaning after 7.5 years have been analysed. This allows a quantification of the aspects of utility, depending on customer behaviour, even though this means quantifying “value choices“. It

A novel electrochemical nanobiosensor for the ultrasensitive and specific detection of femtomolar-level gastric cancer biomarker miRNA-106a

• Maryam Daneshpour,
• Kobra Omidfar and
• Hossein Ghanbarian

Beilstein J. Nanotechnol. 2016, 7, 2023–2036, doi:10.3762/bjnano.7.193

• were 3.2% and 2.2%, respectively, and the inter-assay reproducibility was 5.5% and 4.8%, respectively. These results demonstrate that the fabricated miRNA-nanobiosensor promises an efficient quantification process with trace detection limit and acceptable reproducibility, which is really valuable in

• /SPCEs with the freshly-prepared ones over a 10-week period. As the results show (Figure S4), the response of miRNA-nanobiosensor remained quite stable up to eight weeks and after that, there was a loss in signal strength of about 30% [43][47]. miR-106a quantification in spiked and real samples The

• expression of cancer-associated miRNA-106a in serum can be screened as a valuable non-invasive biomarker of GC. However, quantification methodologies are only useful if they are able to detect miRNA in serum, despite of background noises generated from serum factors. Table 1 shows the analyses of spiked

Chitosan-based nanoparticles for improved anticancer efficacy and bioavailability of mifepristone

• Huijuan Zhang,
• Fuqiang Wu,
• Yazhen Li,
• Xiping Yang,
• Jiamei Huang,
• Tingting Lv,
• Yingying Zhang,
• Jianzhong Chen,
• Haijun Chen,
• Yu Gao,
• Guannan Liu and
• Lee Jia

Beilstein J. Nanotechnol. 2016, 7, 1861–1870, doi:10.3762/bjnano.7.178

• 100 μL of methanol–water solution (50:50 v/v) followed by vortex-mixing for 3 min and then centrifuged at 15,000 rpm for 10 min. Afterwards, a 4 μL aliquot of the supernatant was injected into the chromatographic systems for analysis. Quantification The MIF concentration in plasma was determined using

Surface-enhanced infrared absorption studies towards a new optical biosensor

• Lothar Leidner,
• Julia Stäb,
• Jennifer T. Adam and
• Günter Gauglitz

Beilstein J. Nanotechnol. 2016, 7, 1736–1742, doi:10.3762/bjnano.7.166

• -enhanced infrared absorption (SEIRA); Introduction Optical biosensors play an important role in the detection and quantification of analytes. Among others, some applications include point-of-care testing (POCT), the monitoring of blood, urine, sudor and respiratory air, and the search for metabolites and

• different measurement techniques in order to gain more information. One idea is to operate a Mach–Zehnder interferometer (MZI) in the mid-infrared, thus combining the interferometric information (quantification) with the characteristic fingerprint (identification) of the attached analyte [6]. An example of

• to the mid-infrared regime, the sensitivity to changes in film thickness is reduced compared to the visible wavelength regime, as the mid-IR wavelength regime is larger by a factor of 12–35 as compared to the visible region. Therefore, IR is less suited for detection and quantification of small

Antitumor magnetic hyperthermia induced by RGD-functionalized Fe₃O₄ nanoparticles, in an experimental model of colorectal liver metastases

• Oihane K. Arriortua,
• Eneko Garaio,
• Borja Herrero de la Parte,
• Maite Insausti,
• Luis Lezama,
• Fernando Plazaola,
• Jose Angel García,
• Jesús M. Aizpurua,
• Maialen Sagartzazu,
• Mireia Irazola,
• Nestor Etxebarria,
• Ignacio García-Alonso,
• Alberto Saiz-López and
• José Javier Echevarria-Uraga

Beilstein J. Nanotechnol. 2016, 7, 1532–1542, doi:10.3762/bjnano.7.147

• animals was only raised by 2.4 °C could be due to the dispersion of heat by the blood flow through those parts of the animal not within the focus of the EA. After hyperthermia, the rats were sacrificed and tumor and liver tissue samples were obtained for quantification of iron content and for pathologic

The effect of dry shear aligning of nanotube thin films on the photovoltaic performance of carbon nanotube–silicon solar cells

• Benedikt W. Stolz,
• Daniel D. Tune and
• Benjamin S. Flavel

Beilstein J. Nanotechnol. 2016, 7, 1486–1491, doi:10.3762/bjnano.7.141

• are many (n,m) species in the distribution, with the M11 region also displaying several components. The data is presented in Figure 2a on the familiar absorbance scale but a simple conversion yields the T550 value commonly used in the quantification of transparent films’ performance. The isotropic

An ellipsometric approach towards the description of inhomogeneous polymer-based Langmuir layers

• Falko O. Rottke,
• Burkhard Schulz,
• Klaus Richau,
• Karl Kratz and
• Andreas Lendlein

Beilstein J. Nanotechnol. 2016, 7, 1156–1165, doi:10.3762/bjnano.7.107

• several hundred micrometers. The conversion into thickness maps allows the additional quantification of observed morphologies of certain regions within one map. This helps to investigate and describe inhomogeneous Langmuir layers. Such applications are definitely not limited to polymer-based Langmuir

Voltammetric determination of polyphenolic content in pomegranate juice using a poly(gallic acid)/multiwalled carbon nanotube modified electrode

• Refat Abdel-Hamid and
• Emad F. Newair

Beilstein J. Nanotechnol. 2016, 7, 1104–1112, doi:10.3762/bjnano.7.103

• phosphotungstic blue is relative to the number of aromatic phenolic groups and is used for their quantification, using gallic acid as a standard. An aliquot of 20 μL of the raw juice, 1.58 mL of water and 100 μL of Folin–Ciocalteu reagent was mixed. After waiting 8 min, 300 μL of a solution of sodium carbonate

Advanced atomic force microscopy techniques III

• Thilo Glatzel and
• Thomas Schimmel

Beilstein J. Nanotechnol. 2016, 7, 1052–1054, doi:10.3762/bjnano.7.98

• and compared with theoretical predictions [12][13], and local charges within single molecules can be measured [14][15], however, the quantification is still under intense discussion. Chemical reactions are triggered and imaged by the AFM tip [16][17] and of course the technique is not limited to

Improved biocompatibility and efficient labeling of neural stem cells with poly(L-lysine)-coated maghemite nanoparticles

• Igor M. Pongrac,
• Marina Dobrivojević,
• Lada Brkić Ahmed,
• Michal Babič,
• Miroslav Šlouf,
• Daniel Horák and
• Srećko Gajović

Beilstein J. Nanotechnol. 2016, 7, 926–936, doi:10.3762/bjnano.7.84

• was used. The quantification of cell labeling by cytometry was considered superior to Prussian blue image quantification due to the possible adherence of stain both to the cells and to the coated dish surface. By using flow cytometry and measuring the increase of the side scattered light (SSC) of the

Novel roles for well-known players: from tobacco mosaic virus pests to enzymatically active assemblies

• Claudia Koch,
• Fabian J. Eber,
• Carlos Azucena,
• Alexander Förste,
• Stefan Walheim,
• Thomas Schimmel,
• Alexander M. Bittner,
• Holger Jeske,
• Hartmut Gliemann,
• Sabine Eiben,
• Fania C. Geiger and
• Christina Wege

Beilstein J. Nanotechnol. 2016, 7, 613–629, doi:10.3762/bjnano.7.54

• enabled a colorimetric detection of catalytic activity and the quantification of glucose [152][166][167]. Specifically, GOx catalyzes glucose oxidation to D-glucono-1,5-lactone, thereby producing hydrogen peroxide [168]. This is a substrate for HRP, which reduces it to water, and, as a side reaction, can

Nanoscale effects in the characterization of viscoelastic materials with atomic force microscopy: coupling of a quasi-three-dimensional standard linear solid model with in-plane surface interactions

• Santiago D. Solares

Beilstein J. Nanotechnol. 2016, 7, 554–571, doi:10.3762/bjnano.7.49

• . Regarding the quantification of elastic sample behaviors within periodic dynamic measurements, it is important to point out the two frequency limits for which the response of the 1D SLS model is purely elastic (see Figure 2). At zero frequency (or infinite deformation time scale) the complex modulus is

Hydration of magnesia cubes: a helium ion microscopy study

• Ruth Schwaiger,
• Johannes Schneider,
• Gilles R. Bourret and
• Oliver Diwald

Beilstein J. Nanotechnol. 2016, 7, 302–309, doi:10.3762/bjnano.7.28

• (d–f) 4 days in the HIM chamber (chamber pressure p < 3·10−7 mbar). Significant volume increase, rounding of the edges, and partial fusion of the particles was observed (see arrows in micrographs). (HIM SE images digitally magnified from Figure 1.) Quantification of morphological changes: Curves

Mismatch detection in DNA monolayers by atomic force microscopy and electrochemical impedance spectroscopy

• Maryse D. Nkoua Ngavouka,
• Pietro Capaldo,
• Elena Ambrosetti,
• Giacinto Scoles,
• Loredana Casalis and
• Pietro Parisse

Beilstein J. Nanotechnol. 2016, 7, 220–227, doi:10.3762/bjnano.7.20

• to the electrode surface. Conclusion: We demonstrate here two platforms based on different sensing strategies as sensitive and selective tools to discriminate mismatches. Our assays are ready for parallelization and can be used in the detection and quantification of single nucleotide mismatches in

3D solid supported inter-polyelectrolyte complexes obtained by the alternate deposition of poly(diallyldimethylammonium chloride) and poly(sodium 4-styrenesulfonate)

• Eduardo Guzmán,
• Armando Maestro,
• Sara Llamas,
• Jesús Álvarez-Rodríguez,
• Francisco Ortega,
• Ángel Maroto-Valiente and
• Ramón G. Rubio

Beilstein J. Nanotechnol. 2016, 7, 197–208, doi:10.3762/bjnano.7.18

• temperature [13]. In the last years, a lot of theoretical and experimental research effort has been spent to understand the different growth mechanisms that appear during the alternate deposition of the layers, the quantification of the adsorbed amount of material in each adsorption cycle, as well as the

pH-Triggered release from surface-modified poly(lactic-co-glycolic acid) nanoparticles

• Manuel Häuser,
• Klaus Langer and
• Monika Schönhoff

Beilstein J. Nanotechnol. 2015, 6, 2504–2512, doi:10.3762/bjnano.6.260

• commonly applied to detect anionic polymers [29] or dyes [30]. The obtained release data reveal valuable information about the pH-tunability of PAA in the complexed state within a polyelectrolyte multilayer. The quantification of released PDADMAC is a direct evidence of PEM decomposition and marks their

• . Typical broad polymer resonances are observed, where two characteristic signals for PDADMAC occur at chemical shifts of 3.15 and 3.25 ppm in a region where all other polymers show now signal, which makes a quantification of PDADMAC possible using 1H spectra. Quantification of PEI or PAA is difficult due

• to the comparatively lower signal intensities and some spectral overlap. The reference spectrum of resorcinol (data not shown) shows only chemical shifts higher than 4.8 ppm, which do not interfere with any polymer signal. For quantification, the integral of the resorcinol signal at a chemical shift

A simple and efficient quasi 3-dimensional viscoelastic model and software for simulation of tapping-mode atomic force microscopy

• Santiago D. Solares

Beilstein J. Nanotechnol. 2015, 6, 2233–2241, doi:10.3762/bjnano.6.229

• quantification of tip–sample dissipation in atomic force microscopy (AFM) has been an ongoing subject of interest since the early days of the technique [1][2]. A significant percentage of the surfaces characterized with AFM exhibit rate-dependent deformation processes that result in dissipative tip–sample

Au nanoparticle-based sensor for apomorphine detection in plasma

• Chiara Zanchi,
• Andrea Lucotti,
• Matteo Tommasini,
• Sebastiano Trusso,
• Ugo de Grazia,
• Emilio Ciusani and
• Paolo M. Ossi

Beilstein J. Nanotechnol. 2015, 6, 2224–2232, doi:10.3762/bjnano.6.228

• plasma To prove the applicability of the proposed method to the detection and quantification of APO in blood samples, a few experiments were performed on unfiltered blood plasma with different APO concentrations. These challenging conditions were chosen to minimize sample preparation, while still

Selective porous gates made from colloidal silica nanoparticles

• Roberto Nisticò,
• Paola Avetta,
• Paola Calza,
• Debora Fabbri,
• Giuliana Magnacca and
• Dominique Scalarone

Beilstein J. Nanotechnol. 2015, 6, 2105–2112, doi:10.3762/bjnano.6.215

• . Quantification was performed with external calibration curves (R2 ≥ 0.9999 for MB and R2 ≥ 0.9998 for RNAse, see Figures S1 and S2 in Supporting Information File 1). Additionally, some tests were performed applying an external stimulus (i.e., electric field) by using a battery of 9 V connected with two graphite

Optimized design of a nanostructured SPCE-based multipurpose biosensing platform formed by ferrocene-tethered electrochemically-deposited cauliflower-shaped gold nanoparticles

• Wicem Argoubi,
• Maroua Saadaoui,
• Sami Ben Aoun and
• Noureddine Raouafi

Beilstein J. Nanotechnol. 2015, 6, 1840–1852, doi:10.3762/bjnano.6.187

• recognition sites of the immunosensor. The plot of potential variation vs the amount of Ag gives a straight line (R2 = 0.97871) (Figure 4d) which confirmed the reproducibility of the measurements [35]. The limit of detection (LoD) and the limit of quantification (LoQ) were calculated using the formulae LoD