Instance maps as an organising concept for complex experimental workflows as demonstrated for (nano)material safety research

• Benjamin Punz,
• Maja Brajnik,
• Joh Dokler,
• Jaleesia D. Amos,
• Litty Johnson,
• Katie Reilly,
• Anastasios G. Papadiamantis,
• Amaia Green Etxabe,
• Lee Walker,
• Diego S. T. Martinez,
• Steffi Friedrichs,
• Klaus M. Weltring,
• Nazende Günday-Türeli,
• Claus Svendsen,
• Christine Ogilvie Hendren,
• Mark R. Wiesner,
• Martin Himly,
• Iseult Lynch and
• Thomas E. Exner

Beilstein J. Nanotechnol. 2025, 16, 57–77, doi:10.3762/bjnano.16.7

• functionalised SiO2 nanomaterials under varying conditions. They also compared artificial and real allergen mixtures by applying genuine detection assays suitable for allergenic molecules in vitro and assessed two recently developed in silico protein corona prediction tools regarding the results from

Phospholipid arrays on porous polymer coatings generated by micro-contact spotting

• Sylwia Sekula-Neuner,
• Monica de Freitas,
• Lea-Marie Tröster,
• Tobias Jochum,
• Pavel A. Levkin,
• Michael Hirtz and
• Harald Fuchs

Beilstein J. Nanotechnol. 2017, 8, 715–722, doi:10.3762/bjnano.8.75

• arrays we selected lipid–protein pairs applied in previous settings: Biotin-Cap-PE and streptavidin labeled with Cy3 dye (STV-Cy3) as a simple protein model; and DNP-cap-PE with anti-DNP IgE as a model for allergen/antibody recognition. These interactions are well-characterized for biomimetic lipid

• over the whole arrays, indicating an even distribution of biotin headgroups embedded in the nanoporous HEMA-EDMA (Figure 2). To assess the accessibility of lipid based allergen arrays on nanoporous HEM-EDMA A substrates to antibodies, we employed the established lipid/antibody pair DNP-cap-PE and anti

• -DNP IgE antibody. Previously, DNP-lipid recognition by a specific antibody was tested on glass supports [17], while on HEMA-EDMA polymer antibody binding was tested on covalently bound DNP-azide ink [10]. Here, allergen arrays were prepared by using 10 mol % admixture of DNP-cap-PE into DOPC lipid. As

Comparison of four functionalization methods of gold nanoparticles for enhancing the enzyme-linked immunosorbent assay (ELISA)

• Paula Ciaurriz,
• Fátima Fernández,
• Edurne Tellechea,
• Jose F. Moran and
• Aaron C. Asensio

Beilstein J. Nanotechnol. 2017, 8, 244–253, doi:10.3762/bjnano.8.27

• approach, our data showed a sensitivity increase of at least five times and a lower detection limit with respect to a standard ELISA of at least three times. Additionally, the assay time was remarkably decreased. Keywords: allergen; ELISA enhancement; functionalization; gliadin; gold nanoparticle

• antibodies and HRP on AuNPs. Many of the allergen determinations by ELISA use this strategy, but more research on this is necessary to confirm this. Conclusion In summary, our main objective at the start of the work was to elucidate whether a covalent loading or directional binding of biomolecules on AuNPs