Optical bio/chemical sensors for vitamin B₁₂ analysis in food and pharmaceuticals: state of the art, challenges, and future outlooks

• Seyed Mohammad Taghi Gharibzahedi and
• Zeynep Altintas

Beilstein J. Nanotechnol. 2025, 16, 2207–2244, doi:10.3762/bjnano.16.153

• nanoscale, serve as an advanced spectroscopic platform for improved molecular identification. They highlighted graphene’s potential in biosensing owing to its capability to be functionalized with dissimilar biomolecules such as enzymes and DNA. In their study, they presented a quantitative bioassay

• 53.5 ng/mL using graphene nanoribbons modified with specific recognition elements [96]. Moreover, Bareza et al. demonstrated the scalability and industrial applicability of this bioassay through the use of large-area nanostructured graphene films, proving the promising future of graphene-based mid-IR

Targeting the vector of arboviruses Aedes aegypti with nanoemulsions based on essential oils: a review with focus on larvicidal and repellent properties

• Laryssa Ferreira do Nascimento Silva,
• Douglas Dourado,
• Thayse Silva Medeiros,
• Mariana Alice Gonzaga Gabú,
• Maria Cecilia Queiroga dos Santos,
• Daiane Rodrigues dos Santos,
• Mylena Lemos dos Santos,
• Gabriel Bezerra Faierstein,
• Rosângela Maria Rodrigues Barbosa and
• Fabio Rocha Formiga

Beilstein J. Nanotechnol. 2025, 16, 1894–1913, doi:10.3762/bjnano.16.132

• control. The larvicidal bioassay demonstrated that the concentration of 100 mg/mL resulted in 98% mortality in 24 hours and 100% after 48 hours. The LC50 was 54.23 mg/mL after 24 hours and 46.06 mg/mL at 48 hours. De Oliveira et al. (2020) developed a nanoemulsion containing Piper alatipetiolatum

• ultrasonication (40 kHz). This formulation demonstrated physicochemical stability, with good optical transparency and absence of phase separation. Characterization by TEM revealed spherical droplets with sizes between 20 and 40 nm. In the larvicidal bioassay, fourth-instar larvae of Ae. aegypti were exposed to

Better together: biomimetic nanomedicines for high performance tumor therapy

• Imran Shair Mohammad,
• Gizem Kursunluoglu,
• Anup Kumar Patel,
• Hafiz Muhammad Ishaq,
• Cansu Umran Tunc,
• Dilek Kanarya,
• Mubashar Rehman,
• Omer Aydin and
• Yin Lifang

Beilstein J. Nanotechnol. 2025, 16, 1246–1276, doi:10.3762/bjnano.16.92

• . BSA has some immunogenic effects and is therefore used in immunotherapy and analytic bioassay applications [78][81][82][83]. 1.3.3 Human serum albumin. Human serum albumin (HSA) has biomedical applications as HAS-templated NPs exhibit immune escape, enhanced stability, and high drug loading [84][85

Chitosan nanocomposite containing rotenoids: an alternative bioinsecticidal approach for the management of Aedes aegypti

• Maria A. A. Bertonceli,
• Vitor D. C. Cristo,
• Ivo J. Vieira,
• Francisco J. A. Lemos,
• Arnoldo R. Façanha,
• Raimundo Braz-Filho,
• Gustavo V. T. Batista,
• Luis G. M. Basso,
• Sérgio H. Seabra,
• Thalya S. R. Nogueira,
• Felipe F. Moreira,
• Arícia L. E. M. Assis,
• Antônia E. A. Oliveira and
• Kátia V. S. Fernandes

Beilstein J. Nanotechnol. 2025, 16, 1197–1208, doi:10.3762/bjnano.16.88

• /mL for the controlled ionic gelation method. The larvicidal bioassay of the nanocomposite produced by the controlled ionic gelation method (CS/TPP–β-CD–rot, Figure 5B) revealed a significant increase in larvicidal activity, with an LC50 of 91.69 ppm and an LC90 of 149.06 ppm, indicating higher

• (WHO) for larvicidal bioassays, which applies the cumulative normal distribution model. In contrast, the bioassay conducted with the nanocomposite produced by the conventional ionic gelation method (CS/TPP–rot, Figure 5A) showed an LC50 of 157.02 ppm and an LC90 of 246.52 ppm, a value notably higher

Green synthesis of silver nanoparticles derived from algae and their larvicidal properties to control Aedes aegypti

• Matheus Alves Siqueira de Assunção,
• Douglas Dourado,
• Daiane Rodrigues dos Santos,
• Gabriel Bezerra Faierstein,
• Mara Elga Medeiros Braga,
• Severino Alves Junior,
• Rosângela Maria Rodrigues Barbosa,
• Herminio José Cipriano de Sousa and
• Fábio Rocha Formiga

Beilstein J. Nanotechnol. 2024, 15, 1566–1575, doi:10.3762/bjnano.15.123

• nanomaterials in non-target species is discussed. Keywords: bioassay; inorganic nanoparticles; mosquito vector; nanotechnology; physicochemical; tropical neglected diseases; Introduction Arboviroses are diseases caused by the pathogens transmitted by arthropods, and their transmission to humans occurs through

Development and characterization of potential larvicidal nanoemulsions against Aedes aegypti

• Jonatas L. Duarte,
• Leonardo Delello Di Filippo,
• Anna Eliza Maciel de Faria Mota Oliveira,
• Rafael Miguel Sábio,
• Gabriel Davi Marena,
• Tais Maria Bauab,
• Cristiane Duque,
• Vincent Corbel and
• Marlus Chorilli

Beilstein J. Nanotechnol. 2024, 15, 104–114, doi:10.3762/bjnano.15.10

• involving Galleria mellonella larvae. Additionally, we investigated the insecticidal efficacy of monoterpenes against the mosquito Aedes aegypti, the primary dengue vector, via larval bioassay. Employing a low-energy approach, we successfully generated nanoemulsions. The cymene-based nanoemulsion exhibited

Cardiomyocyte uptake mechanism of a hydroxyapatite nanoparticle mediated gene delivery system

• Hiroaki Komuro,
• Masahiro Yamazoe,
• Kosuke Nozaki,
• Akiko Nagai and
• Tetsuo Sasano

Beilstein J. Nanotechnol. 2020, 11, 1685–1692, doi:10.3762/bjnano.11.150

• 0.45 µm filter to remove aggregated nanoparticles. The concentration of the filtered solutions was measured using a calcium assay kit in accordance with the guidelines of the manufacturer (BioAssay Systems). TEM was performed to observe the size and shape of HAp nanoparticles at an acceleration voltage

Plant growth regulation by seed coating with films of alginate and auxin-intercalated layered double hydroxides

• Vander A. de Castro,
• Valber G. O. Duarte,
• Danúbia A. C. Nobre,
• Geraldo H. Silva,
• Vera R. L. Constantino,
• Frederico G. Pinto,
• Willian R. Macedo and
• Jairo Tronto

Beilstein J. Nanotechnol. 2020, 11, 1082–1091, doi:10.3762/bjnano.11.93

• germination rate and germination speed index of the seeds, as well as biometric analyses including measurements of root area, root fresh matter, and shoot length of the plants. The bioassay performed in soil pots showed that the alginate film containing ZnAl-NAA-LDH yields an enhancement regarding root area

• efficiency, which is of particular relevance for tropical weathered soils [10]. A kinetic study of phosphorus release and a bioassay under controlled conditions using maize (Zea mays) as indicator plant were performed to test the suitability of the proposed material. The results obtained using LDH-phosphate

• were compared to those of commercial triple superphosphate (TSP) fertilizer in two different soils, namely, a sandy soil and a clayey soil. Under the experimental conditions of the bioassay, plant productivity, height, and the content of phosphorus in the dry matter increased as a result of applying

Using gold nanoparticles to detect single-nucleotide polymorphisms: toward liquid biopsy

• María Sanromán Iglesias and
• Marek Grzelczak

Beilstein J. Nanotechnol. 2020, 11, 263–284, doi:10.3762/bjnano.11.20

• aggregation of the two DNA–AuNP complexes. The catalyst was released back into the solution. The authors observed that the aggregation of nanoparticles was slower by a factor of 10 when the sequence of the catalyst contained single-base mismatches (spurious catalyst). This colorimetric bioassay could detect

Comparison of four methods for the biofunctionalization of gold nanorods by the introduction of sulfhydryl groups to antibodies

• Xuefeng Wang,
• Zhong Mei,
• Yanyan Wang and
• Liang Tang

Beilstein J. Nanotechnol. 2017, 8, 372–380, doi:10.3762/bjnano.8.39

• bioassay. We further evaluated non-specific binding by probing rabbit IgG and other non-target proteins. Figures S6–S9 (Supporting Information File 1) show the LSPR responses after the biochip was exposed to rabbit IgG (3 mg/mL), myoglobin (1 μg/mL), and cardiac troponin I (1 μg/mL). Consistent with the

Release behaviour and toxicity evaluation of levodopa from carboxylated single-walled carbon nanotubes

• Julia M. Tan,
• Jhi Biau Foo,
• Sharida Fakurazi and
• Mohd Zobir Hussein

Beilstein J. Nanotechnol. 2015, 6, 243–253, doi:10.3762/bjnano.6.23

• nanohybrid possessed favourable sustained and controlled release properties as a drug carrier. In vitro bioassay PC12 cell lines PC12 is one of the most widely applied neuronal cell lines and can be used as a model to study secretory activity and catecholamine metabolism and regulation. In this study, we

• novelty for further development as a slow, sustained-release formulation. The MTT bioassay results showed a dose-dependent cytotoxicity response from pure LD, whereas SWCNT–COOH and SWCNT–LD did not compromise the viability of PC12 cells, which remained almost constant throughout the experiments. This

Sorting of droplets by migration on structured surfaces

• Wilfried Konrad and
• Anita Roth-Nebelsick

Beilstein J. Nanotechnol. 2011, 2, 215–221, doi:10.3762/bjnano.2.25

• -chip” concepts aim at integrating chemical and biochemical processes into chip-like designs that enable the user to carry out tasks in analytical chemistry or bioassay applications [2]. The design of microfluidic batch processes requires a continuous and controlled flow and cycling of suspended