Influence of surface characteristics on the in vitro stability and cell uptake of nanoliposomes for brain delivery

• Dushko Shalabalija,
• Ljubica Mihailova,
• Nikola Geskovski,
• Andreas Zimmer,
• Otmar Geiss,
• Sabrina Gioria,
• Diletta Scaccabarozzi and
• Marija Glavas Dodov

Beilstein J. Nanotechnol. 2026, 17, 139–158, doi:10.3762/bjnano.17.9

• neurons (ranging from 25.17% to 27.54%). Fluorescence and confocal microscopy micrographs revealed that, once internalized, NLs were concentrated in the perinuclear cell regions. Keywords: blood–brain barrier; cell co-culture; cell uptake; internalization; nanoliposomes; stability; surface

Nanoparticle delivery to metastatic breast cancer cells by nanoengineered mesenchymal stem cells

• Liga Saulite,
• Karlis Pleiko,
• Ineta Popena,
• Dominyka Dapkute,
• Ricardas Rotomskis and
• Una Riekstina

Beilstein J. Nanotechnol. 2018, 9, 321–332, doi:10.3762/bjnano.9.32

• , Vilnius University, Sauletekio Ave. 7, LT-10257 Vilnius, Lithuania, Laser Research Centre, Vilnius University, Sauletekio al. 9, corp. 3, LT-10222 Vilnius, Lithuania 10.3762/bjnano.9.32 Abstract We created a 3D cell co-culture model by combining nanoengineered mesenchymal stem cells (MSCs) with the

• ]. However, after 48 h, 72 h and 96 h propagation on polyHEMA coatings, CD90 expression was reduced to 92%, 81% and 88%, respectively (Figure 2C). Therefore, we chose 24 h as the optimal incubation time for 3D cell co-culture experiments to ensure the selectivity of the CD90 marker towards MSCs. Quantum dot

• . Endocytosis inhibitor assay; QD endocytic pathway analysis in MSCs, MCF7 and MDA-MB-231 cells; Confocal microscopy z-sections of MSC and breast cancer cell co-culture spheroids; EpCAM expression in MSCs and breast cancer cells MCF7 and MDA-MB-231; QD transfer from nanoengineered MSCs to MCF7 cells using EpCAM

Atomic force microscopy as analytical tool to study physico-mechanical properties of intestinal cells

• Christa Schimpel,
• Oliver Werzer,
• Eleonore Fröhlich,
• Gerd Leitinger,
• Markus Absenger-Novak,
• Birgit Teubl,
• Andreas Zimmer and
• Eva Roblegg

Beilstein J. Nanotechnol. 2015, 6, 1457–1466, doi:10.3762/bjnano.6.151

• determined. For all calculations a cantilever spring constant of 0.1 N/m was assumed (specified by the manufactures). A matlab program based on Butt et al. [65] was used for data handling and plotting. SEM analyses of the Caco-2 monolayer (A, B) and the Caco-2/M cell co-culture (C, D). The most prominent

PVP-coated, negatively charged silver nanoparticles: A multi-center study of their physicochemical characteristics, cell culture and in vivo experiments

• Sebastian Ahlberg,
• Alexandra Antonopulos,
• Jörg Diendorf,
• Ralf Dringen,
• Matthias Epple,
• Rebekka Flöck,
• Wolfgang Goedecke,
• Christina Graf,
• Nadine Haberl,
• Jens Helmlinger,
• Fabian Herzog,
• Frederike Heuer,
• Stephanie Hirn,
• Christian Johannes,
• Stefanie Kittler,
• Manfred Köller,
• Katrin Korn,
• Wolfgang G. Kreyling,
• Fritz Krombach,
• Jürgen Lademann,
• Kateryna Loza,
• Eva M. Luther,
• Marcelina Malissek,
• Martina C. Meinke,
• Daniel Nordmeyer,
• Anne Pailliart,
• Jörg Raabe,
• Fiorenza Rancan,
• Barbara Rothen-Rutishauser,
• Eckart Rühl,
• Carsten Schleh,
• Andreas Seibel,
• Christina Sengstock,
• Lennart Treuel,
• Annika Vogt,
• Katrin Weber and
• Reinhard Zellner

Beilstein J. Nanotechnol. 2014, 5, 1944–1965, doi:10.3762/bjnano.5.205

Mimicking exposures to acute and lifetime concentrations of inhaled silver nanoparticles by two different in vitro approaches

• Fabian Herzog,
• Kateryna Loza,
• Sandor Balog,
• Martin J. D. Clift,
• Matthias Epple,
• Peter Gehr,
• Alke Petri-Fink and
• Barbara Rothen-Rutishauser

Beilstein J. Nanotechnol. 2014, 5, 1357–1370, doi:10.3762/bjnano.5.149

• interaction with Ag NPs can occur through the lung, skin, gastrointestinal tract, and bloodstream. However, the inhalation of Ag NP aerosols is a primary concern. To study the possible effects of inhaled Ag NPs, an in vitro triple cell co-culture model of the human alveolar/airway barrier (A549 epithelial

• layer of the triple cell co-culture model, which is a similar pattern as we have observed for Ag NPs exposed to cells at the ALI [44]. To reduce misinterpretation due to staining artefacts [48], samples were treated with uranyl acetate only, without lead citrate. Cytotoxicity As described in [44], we

• ]. Furthermore, the triple cell co-culture system has been evaluated in terms of its functional relevance in vivo and also allows studies at the ALI [40][61]. Only a few studies investigated the effects of Ag NPs at the ALI [44][57][62]. We have used the same cell cultures and similar endpoints as described in