Attempts to preserve and visualize protein corona on the surface of biological nanoparticles in blood serum using photomodification

• Julia E. Poletaeva,
• Anastasiya V. Tupitsyna,
• Alina E. Grigor’eva,
• Ilya S. Dovydenko and
• Elena I. Ryabchikova

Beilstein J. Nanotechnol. 2024, 15, 1654–1666, doi:10.3762/bjnano.15.130

• obtained direct images of a “natural” protein corona on natural bio-NPs of blood serum for the first time Keywords: chylomicrons; extracellular vesicles; lipoproteins; photomodification; protein corona; Introduction The existence of a protein corona on all nanoparticles (NPs) entering biological fluids

• unexpected finding in the FBS-derived samples were rare chylomicrons that appeared deformed (Figure 6g). Fine changes in ultrastructure noted in control samples isolated on a sucrose gradient (see above) were also detected after photomodification. For example, the loosening of a LP surface is clearly seen in

• samples, chylomicrons were observed (Figure 6k,l), the content of which was higher than in the samples obtained from FBS. Chylomicrons were located in small areas of the grid that were free of contamination. They had a low-electron-density envelope, to which globules (Figure 6k) and accumulations of a

The impact of molecular tumor profiling on the design strategies for targeting myeloid leukemia and EGFR/CD44-positive solid tumors

• Nikola Geskovski,
• Nadica Matevska-Geshkovska,
• Simona Dimchevska Sazdovska,
• Marija Glavas Dodov,
• Kristina Mladenovska and
• Katerina Goracinova

Beilstein J. Nanotechnol. 2021, 12, 375–401, doi:10.3762/bjnano.12.31

• macrophages protrude through the vascular endothelial wall to gain access and monitor blood circulation [24]. Hussain et al demonstrated that perisinusoidal macrophages are responsible for the accumulation of the chylomicrons in the BM and, hence, play a crucial role in the delivery of lipids, as a source of