Synthetic-polymer-assisted antisense oligonucleotide delivery: targeted approaches for precision disease treatment

• Ana Cubillo Alvarez,
• Dylan Maguire and
• Ruairí P. Brannigan

Beilstein J. Nanotechnol. 2025, 16, 435–463, doi:10.3762/bjnano.16.34

• optimise ASO-based therapeutics for more precise and effective disease treatments. Keywords: antisense oligonucleotides; enhanced delivery; gene transfection; intracellular uptake; locked nucleic acid (LNA); nanoparticles; peptide nucleic acid (PNA); personalised therapy; phosphorodiamidate morpholino

Recent updates in applications of nanomedicine for the treatment of hepatic fibrosis

• Damai Ria Setyawati,
• Fransiska Christydira Sekaringtyas,
• Riyona Desvy Pratiwi,
• A’liyatur Rosyidah,
• Rohimmahtunnissa Azhar,
• Nunik Gustini,
• Gita Syahputra,
• Idah Rosidah,
• Etik Mardliyati,
• Tarwadi and
• Sjaikhurrizal El Muttaqien

Beilstein J. Nanotechnol. 2024, 15, 1105–1116, doi:10.3762/bjnano.15.89

• uptake in a time-dependent manner. Ultimately, the nanocomplex showed excellent in vitro gene-silencing activities, that is, approximately 80–85% of the Pcbp2 mRNA expression was inhibited in activated HSCs-T6 cells after gene transfection for 24 h. The in vivo biodistribution showed that the nanocomplex

Recent progress in cancer cell membrane-based nanoparticles for biomedical applications

• Qixiong Lin,
• Yueyou Peng,
• Yanyan Wen,
• Xiaoqiong Li,
• Donglian Du,
• Weibin Dai,
• Wei Tian and
• Yanfeng Meng

Beilstein J. Nanotechnol. 2023, 14, 262–279, doi:10.3762/bjnano.14.24

• major organs [115]. miRNA365 has been confirmed to inhibit tumor cell development and promote tumor cell apoptosis in the colon and other tumors [116]. Zhang et al. achieved efficient gene transfection in tumor tissue by preparing homologous colon cancer membrane-coated biomimetic NPs [55]. These

Cyclodextrins as eminent constituents in nanoarchitectonics for drug delivery systems

• Makoto Komiyama

Beilstein J. Nanotechnol. 2023, 14, 218–232, doi:10.3762/bjnano.14.21

• applications. However, nucleic acids are intrinsically unstable in serum and do not readily cross the cellular plasma membranes. Accordingly, it is a big challenge to deliver them in the intact form into target cells. Conventional gene transfection reagents are not very good candidates, since multiple positive

Theranostic potential of self-luminescent branched polyethyleneimine-coated superparamagnetic iron oxide nanoparticles

• Rouhollah Khodadust,
• Ozlem Unal and
• Havva Yagci Acar

Beilstein J. Nanotechnol. 2022, 13, 82–95, doi:10.3762/bjnano.13.6

• , Uskudar, Istanbul, Turkey Koc University, Graduate School of Materials Science and Engineering, Rumelifeneri Yolu, Sariyer, Istanbul, Turkey 10.3762/bjnano.13.6 Abstract Polyethylenimine (PEI), which is frequently used for polyplex formation and effective gene transfection, is rarely recognized as a

• partial PEI oxidation during the synthesis. Here, we demonstrate in vitro dye-free optical imaging and successful gene transfection with luminescent SPION@bPEI, which was further modified for receptor-mediated delivery of the cargo selectively to cancer cell lines overexpressing the epidermal growth

• when attached to the iron oxide surface since SPIONs have strong absorption in the UV and visible range of the spectrum [33]. Alternatively, PEI-bound luminescent nanoparticles, such as quantum dots or graphene nanoparticles, are also being studied to combine optical imaging and gene transfection

Comprehensive review on ultrasound-responsive theranostic nanomaterials: mechanisms, structures and medical applications

• Sepand Tehrani Fateh,
• Lida Moradi,
• Elmira Kohan,
• Michael R. Hamblin and
• Amin Shiralizadeh Dezfuli

Beilstein J. Nanotechnol. 2021, 12, 808–862, doi:10.3762/bjnano.12.64

• destroyed with a high-intensity burst of US. Thus, they can locally release the loaded drugs and enhance the penetration depth of the therapeutic agents into the targeted tissue via microstreaming and ARF [72][83][126][168]. Microbubbles can improve the efficacy of gene transfection, therapeutic agents, and

Recent progress in magnetic applications for micro- and nanorobots

• Ke Xu,
• Shuang Xu and
• Fanan Wei

Beilstein J. Nanotechnol. 2021, 12, 744–755, doi:10.3762/bjnano.12.58

• -assisted gene transfection. Magnetic MNRs containing nucleic acids were delivered to target cells by magnetic fields. This method could change gene function or protein expression, which is of great significance for future research on gene transfer and gene therapy. Table 1 summarizes the relevant

Cardiomyocyte uptake mechanism of a hydroxyapatite nanoparticle mediated gene delivery system

• Hiroaki Komuro,
• Masahiro Yamazoe,
• Kosuke Nozaki,
• Akiko Nagai and
• Tetsuo Sasano

Beilstein J. Nanotechnol. 2020, 11, 1685–1692, doi:10.3762/bjnano.11.150

• , this method may provide a solution to the current problem of using calcium phosphate. The cellular uptake performance is important for a successful vector-mediated gene transfection. In the cellular uptake process, the internalization pathway is an essential factor to prevent the fate of lysosomal

• viability were observed among the three concentrations of HAp/pDNA complexes used at 24 and 72 h (Figure 2). The results suggested that HAp exhibits little cytotoxicity within the concentration range used in this study. Transfection efficiency To test the gene transfection potential of the HAp nanoparticle

• . Discussion The development of gene therapy is essential for generating new treatment options for cardiovascular disease. We focused on safe non-viral vectors using nanotechnology. Although nanoparticle-based gene-transfection methods have been proposed for gene delivery into target cells and tissues, there

Applications of superparamagnetic iron oxide nanoparticles in drug and therapeutic delivery, and biotechnological advancements

• Maria Suciu,
• Corina M. Ionescu,
• Alexandra Ciorita,
• Septimiu C. Tripon,
• Dragos Nica,
• Hani Al-Salami and
• Lucian Barbu-Tudoran

Beilstein J. Nanotechnol. 2020, 11, 1092–1109, doi:10.3762/bjnano.11.94

• microscopy (EM), iron oxide magnetic beads for the separation of cells and molecules, gold and silver nanoparticles as fiducials for EM, for immuno-EM labeling and surface-enhanced Raman spectroscopy, or for gene transfection, liposomes for drug delivery, and gadolinium or iron oxide nanoparticles for

Poly(1-vinylimidazole) polyplexes as novel therapeutic gene carriers for lung cancer therapy

• Gayathri Kandasamy,
• Elena N. Danilovtseva,
• Vadim V. Annenkov and
• Uma Maheswari Krishnan

Beilstein J. Nanotechnol. 2020, 11, 354–369, doi:10.3762/bjnano.11.26

• –imidazole polyamide system was found to inhibit prostate cancer progression through interfering with the expression and function of the androgen receptor [13]. Chitosan–imidazole derivatives have been also explored for gene transfection in HEK293 cells [14]. In recent years, poly(vinylimidazole)-based

• -vinylimidazole) chains modified with aminoethyl groups demonstrated excellent DNA binding ability in synergy with lactosylated poly(ʟ-lysine). This system was found to exhibit excellent gene transfection ability specifically in hepatocytes through interactions with the asialoglycoprotein receptor expressed on

Molecular architectonics of DNA for functional nanoarchitectures

• Debasis Ghosh,
• Lakshmi P. Datta and
• Thimmaiah Govindaraju

Beilstein J. Nanotechnol. 2020, 11, 124–140, doi:10.3762/bjnano.11.11

• nanoarchitectures and cation-free gene transfection ability. Small-molecule-templated DNA nanoarchitectures The molecular self-assembly of DNA through sequence-specific base pairing is extensively used to create complex nanoarchitectures with variable size and shape in the field of classical DNA nanotechnology. In

Layered double hydroxide/sepiolite hybrid nanoarchitectures for the controlled release of herbicides

• Ediana Paula Rebitski,
• Margarita Darder and
• Pilar Aranda

Beilstein J. Nanotechnol. 2019, 10, 1679–1690, doi:10.3762/bjnano.10.163

• components in diverse electrochemical devices (such as supercapacitors, sensors, and biosensors), in drug delivery and controlled-release formulations, or in non-viral gene transfection [21][22][23][24][25][26]. The fact that the stability of LDH varies with the pH value has proved advantageous in some of

Characterization and influence of hydroxyapatite nanopowders on living cells

• Przemyslaw Oberbek,
• Tomasz Bolek,
• Adrian Chlanda,
• Seishiro Hirano,
• Sylwia Kusnieruk,
• Julia Rogowska-Tylman,
• Ganna Nechyporenko,
• Viktor Zinchenko,
• Wojciech Swieszkowski and
• Tomasz Puzyn

Beilstein J. Nanotechnol. 2018, 9, 3079–3094, doi:10.3762/bjnano.9.286

• regenerative medicine. The use of nanosized hydroxyapatites in biomedical applications is constantly growing due to their good mechanical properties and enhanced efficiency of gene transfection in drug delivery. Calcium phosphates are sensitive to the preparation conditions [11][12][13][14][15]. They can be

• delivery or more effective gene transfection based on hydroxyapatite. The degree of crystallinity can be a factor in determining how long an agglomerate will stay inside the cell and what will be the drug-release rate. Hydroxyapatites with exceptionally large surface area could be also used for

Pulmonary surfactant augments cytotoxicity of silica nanoparticles: Studies on an in vitro air–blood barrier model

• Jennifer Y. Kasper,
• Lisa Feiden,
• Maria I. Hermanns,
• Christoph Bantz,
• Michael Maskos,
• Ronald E. Unger and
• C. James Kirkpatrick

Beilstein J. Nanotechnol. 2015, 6, 517–528, doi:10.3762/bjnano.6.54

• positively charged amino-functionalisation regularly displayed higher cytotoxicity (compared to the carboxyl functionalization), while the negatively charged carboxyl-functionalisation proved to be mostly non-toxic [21][22][23]. NPs with a positively charged surface are usually applied in nonviral gene

• transfection and delivery studies using mainly cationic polymers or liposomes [8][24]. The positive NP surface charge enables better cellular contact and/or uptake than negatively charged or neutral molecules [25]. Nevertheless, the use of these positively charged drug and gene delivery carriers remains