Nanotechnological approaches for efficient N2B delivery: from small-molecule drugs to biopharmaceuticals

• Selin Akpinar Adscheid,
• Akif E. Türeli,
• Nazende Günday-Türeli and
• Marc Schneider

Beilstein J. Nanotechnol. 2024, 15, 1400–1414, doi:10.3762/bjnano.15.113

• monoclonal antibody onto the NPs led to an increase in particle size and influenced the distribution of NPs in the hippocampus, indicating another critical factor for N2B delivery, namely, the particle size of NPs [153]. In another study with mAbs, Ferreira et al. developed and optimized a system of PLGA and

• oligomeric chitosan for the co-delivery of alpha-cyano-4-hydroxycinnamic acid and the monoclonal antibody cetuximab to the brain for glioblastoma therapy. The cetuximab conjugation on the NP surface improved the cytotoxicity profile, and a chicken chorioallantoic membrane assay showed enhanced antiangiogenic

Antibody-conjugated nanoparticles for target-specific drug delivery of chemotherapeutics

• Mamta Kumari,
• Amitabha Acharya and
• Praveen Thaggikuppe Krishnamurthy

Beilstein J. Nanotechnol. 2023, 14, 912–926, doi:10.3762/bjnano.14.75

• the theranostic applications of ACNPs for the treatment of cancer. Keywords: active targeting; chemical conjugation; chemotherapeutics; drug delivery; monoclonal antibody; Introduction Off-target side effects, such as myelosuppression, mucositis, alopecia, organ dysfunction, and thrombocytopenia

Metal-organic framework-based nanomaterials as opto-electrochemical sensors for the detection of antibiotics and hormones: A review

• Akeem Adeyemi Oladipo,
• Saba Derakhshan Oskouei and
• Mustafa Gazi

Beilstein J. Nanotechnol. 2023, 14, 631–673, doi:10.3762/bjnano.14.52

Polymer nanoparticles from low-energy nanoemulsions for biomedical applications

• Santiago Grijalvo and
• Carlos Rodriguez-Abreu

Beilstein J. Nanotechnol. 2023, 14, 339–350, doi:10.3762/bjnano.14.29

• were functionalized with the monoclonal antibody F19 via carbodiimide conjugation. This approach enabled the use of ethyl cellulose nanoparticles in bioanalytical applications with the aim to detect Yersinia pestis from direct agglutination tests. Irradiation of nanoparticles under UV light favored

Microneedle-based ocular drug delivery systems – recent advances and challenges

• Piotr Gadziński,
• Anna Froelich,
• Monika Wojtyłko,
• Antoni Białek,
• Julia Krysztofiak and
• Tomasz Osmałek

Beilstein J. Nanotechnol. 2022, 13, 1167–1184, doi:10.3762/bjnano.13.98

• injection [177]. Amer and Chen fabricated PVA hydrogel-based microneedle arrays for the delivery of immunoglobin G1, a model protein resembling bevacizumab, a monoclonal antibody applied in the treatment of age-related macular degeneration (AMD) (Figure 6). First, the master mold was produced with the use

• MeHA shell acted as a depot form. Describing in vivo experiments with the use of animal (rat) corneal neovascularization as a disease model, the authors presented that delivering an anti-angiogenic monoclonal antibody (DC101) by such an eye patch caused a reduction of the area of neovascularization by

Theranostic potential of self-luminescent branched polyethyleneimine-coated superparamagnetic iron oxide nanoparticles

• Rouhollah Khodadust,
• Ozlem Unal and
• Havva Yagci Acar

Beilstein J. Nanotechnol. 2022, 13, 82–95, doi:10.3762/bjnano.13.6

• cytotoxicity at the applied dose (0.62 μg/mL) in either of the cell lines studied, which was desired in this study since Erb was only used to demonstrate molecular targeting. Although HCT116 cells are EGFR positive, the IC50 of the Erb monoclonal antibody is approx. 380 μg/mL [63] and, hence, no significant

Comprehensive review on ultrasound-responsive theranostic nanomaterials: mechanisms, structures and medical applications

• Sepand Tehrani Fateh,
• Lida Moradi,
• Elmira Kohan,
• Michael R. Hamblin and
• Amin Shiralizadeh Dezfuli

Beilstein J. Nanotechnol. 2021, 12, 808–862, doi:10.3762/bjnano.12.64

The impact of molecular tumor profiling on the design strategies for targeting myeloid leukemia and EGFR/CD44-positive solid tumors

• Nikola Geskovski,
• Nadica Matevska-Geshkovska,
• Simona Dimchevska Sazdovska,
• Marija Glavas Dodov,
• Kristina Mladenovska and
• Katerina Goracinova

Beilstein J. Nanotechnol. 2021, 12, 375–401, doi:10.3762/bjnano.12.31

• used as ligands for targeting diagnostic or therapeutic nanoparticles. Qian et al. used cetuximab (C225 mAbs), an EGFR-neutralizing monoclonal antibody for cancer treatment, as a targeting ligand for AuNPs [74]. The authors evaluated the efficacy of C225 mAbs to target EGFR and improve the

Use of data processing for rapid detection of the prostate-specific antigen biomarker using immunomagnetic sandwich-type sensors

• Camila A. Proença,
• Tayane A. Freitas,
• Thaísa A. Baldo,
• Elsa M. Materón,
• Flávio M. Shimizu,
• Gabriella R. Ferreira,
• Frederico L. F. Soares,
• Ronaldo C. Faria and
• Osvaldo N. Oliveira Jr.

Beilstein J. Nanotechnol. 2019, 10, 2171–2181, doi:10.3762/bjnano.10.210

• immobilized on the electrode surface. Also, the monoclonal antibody provides high specificity to a single epitope, which is reflected in a low cross-reactivity. A comparison of various sandwich-type immunosensors and immunoassays for detection of PSA in the literature is presented in Table 1. Repeatability is

• and collaborators [57]. The experimental details are given in Supporting Information File 1. The fabrication of this sandwich-type immunosensor comprises four steps, as depicted in Figure 6: (1) deposition of monoclonal antibody on the carbon electrode, (2) bioconjugate modification using HRP and

• μg·mL−1 monoclonal antibody (Ab1) using 5 μL of 2 mg·mL−1 PDDA and 5 μL of AuNP-GSH. To activate the carboxyl groups from AuNP-GSH and ensure a stable covalent binding of antibodies, EDC/NHS was used (0.4 mol·L−1 EDC and 0.1 mol·L−1 NHS). The electrodes were washed with 1.0 mL of PBS buffer pH 7.4 and

Engineered superparamagnetic iron oxide nanoparticles (SPIONs) for dual-modality imaging of intracranial glioblastoma via EGFRvIII targeting

• Xianping Liu,
• Chengjuan Du,
• Haichun Li,
• Ting Jiang,
• Zimiao Luo,
• Zhiqing Pang,
• Daoying Geng and
• Jun Zhang

Beilstein J. Nanotechnol. 2019, 10, 1860–1872, doi:10.3762/bjnano.10.181

• -HFLIIGFMRRAACGA) peptide and 5-FAM-labeled PEPHC1 (5-FAM-C6-HFLIIGFMRRAACGA) peptide were synthesized by the Chinese Peptide Company (Hangzhou, China). EGFRvIII (D6T2Q) XPRabbit monoclonal antibody was purchased from Cell Signaling Technology (USA). Alexa Fluor 647-conjugated donkey-anti-rabbit secondary antibody

• into 24-well plates and maintained for 24 h. When the cells reached 80% confluence, they were rinsed with PBS, then stained with EGF Receptor vIII rabbit monoclonal antibody (1 µg/mL) and Alexa Fluor 647-conjugated donkey-anti-rabbit secondary antibody (15 µg/mL). After completing the above steps, a

Advanced atomic force microscopy techniques III

• Thilo Glatzel and
• Thomas Schimmel

Beilstein J. Nanotechnol. 2016, 7, 1052–1054, doi:10.3762/bjnano.7.98

• interaction in liquid media [28]. A software package, dForce, was also presented that allows for a better understanding of amplitude modulation and bimodal AFM experiments in air or liquid [29]. A method for the calibration of the torsional force by using human fibronectin and its monoclonal antibody was

Probing fibronectin–antibody interactions using AFM force spectroscopy and lateral force microscopy

• Andrzej J. Kulik,
• Małgorzata Lekka,
• Kyumin Lee,
• Grazyna Pyka-Fościak and
• Wieslaw Nowak

Beilstein J. Nanotechnol. 2015, 6, 1164–1175, doi:10.3762/bjnano.6.118

• its monoclonal antibody. The results obtained using LFM and AFM-based classical force spectroscopies showed similar unbinding forces recorded at similar loading rates. Our studies verify that the proposed lateral force calibration method can be applied to study single molecule interactions. Keywords

• molecular interactions, we have measured interaction forces between protein fibronectin (FN) and monoclonal antibody against FN (FN-Mab) using both AFM-FS and LFM techniques. The relation between the unbinding force and the loading rate obtained by AFM-FS was compared with the corresponding relation

• the unbinding force between fibronectin and monoclonal antibody were carried out. We assume that independent of the applied unbinding direction (i.e., normal or lateral), both methods (AFM-FS and LFM) provide similar values of the interaction force at similar loading rates. In the AFM-FS method, force

PVP-coated, negatively charged silver nanoparticles: A multi-center study of their physicochemical characteristics, cell culture and in vivo experiments

• Sebastian Ahlberg,
• Alexandra Antonopulos,
• Jörg Diendorf,
• Ralf Dringen,
• Matthias Epple,
• Rebekka Flöck,
• Wolfgang Goedecke,
• Christina Graf,
• Nadine Haberl,
• Jens Helmlinger,
• Fabian Herzog,
• Frederike Heuer,
• Stephanie Hirn,
• Christian Johannes,
• Stefanie Kittler,
• Manfred Köller,
• Katrin Korn,
• Wolfgang G. Kreyling,
• Fritz Krombach,
• Jürgen Lademann,
• Kateryna Loza,
• Eva M. Luther,
• Marcelina Malissek,
• Martina C. Meinke,
• Daniel Nordmeyer,
• Anne Pailliart,
• Jörg Raabe,
• Fiorenza Rancan,
• Barbara Rothen-Rutishauser,
• Eckart Rühl,
• Carsten Schleh,
• Andreas Seibel,
• Christina Sengstock,
• Lennart Treuel,
• Annika Vogt,
• Katrin Weber and
• Reinhard Zellner

Beilstein J. Nanotechnol. 2014, 5, 1944–1965, doi:10.3762/bjnano.5.205

• nanoparticle-treated cells have been stained after 10 h and 22 h with a monoclonal antibody recognizing γ-H2AX. The quantifications of the number of foci induced in the three cell lines CHO9, K1 and V79B are given in Figure 14. About 100 nuclei were scored at each time point, and the number of foci in each

Magnetic nanoparticles for biomedical NMR-based diagnostics

• Huilin Shao,
• Tae-Jong Yoon,
• Monty Liong,
• Ralph Weissleder and
• Hakho Lee

Beilstein J. Nanotechnol. 2010, 1, 142–154, doi:10.3762/bjnano.1.17

• spiked into sputum samples. Following liquefaction, the biological samples were incubated with cannonballs conjugated to an anti-BCG monoclonal antibody. Unbound MNPs were then removed via a built-in membrane filter, embedded within the device (Figure 6c). This membrane (~100 nm size cut-off) not only