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Search for "polyplexes" in Full Text gives 9 result(s) in Beilstein Journal of Nanotechnology.
Synthetic-polymer-assisted antisense oligonucleotide delivery: targeted approaches for precision disease treatment
Beilstein J. Nanotechnol. 2025, 16, 435–463, doi:10.3762/bjnano.16.34
- oligomer (PMO); phosphorothioate (PS); polyplexes; ribose substitutions; small interfering RNA (siRNA); synthetic polymers; tricyclo-DNA (tcDNA); Introduction The development and use of personalised therapies tailored to individual patients have emerged as a powerful strategy for treating various
- . Additionally, the attachment of hydrophobic moieties, amino acids, and small peptides to linear and branched PEI has been explored as an alternative to improve the biostability and bioavailability of cationic polyplexes while offering a targeted delivery with reduced cytotoxicity that is frequently associated
- and coworkers synthesised a library of bPEI polyplexes grafted with hydrophobic ligands including aliphatic alkanes, fluoroalkanes, and cycloalkanes of varying lengths and analysed the structure–activity relationship of these compounds to deliver anti-luciferase siRNA oligonucleotides [119]. All PEI
Nanocarriers and macrophage interaction: from a potential hurdle to an alternative therapeutic strategy
Beilstein J. Nanotechnol. 2025, 16, 97–118, doi:10.3762/bjnano.16.10
Nanotechnological approaches for efficient N2B delivery: from small-molecule drugs to biopharmaceuticals
Beilstein J. Nanotechnol. 2024, 15, 1400–1414, doi:10.3762/bjnano.15.113
- studying single gene function through N2B delivery [156]. In order to deliver RNA efficiently to the brain, different DDSs, such as exosomes, lipid NPs, lipoplexes, and polyplexes, were formulated, and their efficiency to successfully deliver RNA were tested in vivo (Figure 5) [157]. For example, chitosan
- another widely used complex vector, polyplexes. They took the approach one step further and combined these polyplexes formed by a cationic polymer and anionic mRNA with a photosensitizer for photochemical internalization and subsequent enhancement in delivery to the cytoplasm. They showed that laser
- irradiation increased the mRNA expression in the cytoplasm [160]. In addition to polyplexes, lipoplexes, another widely used vector, have also been studied for siRNA delivery. For instance, Hu et al. described the encapsulation of siRNA (specifically of c-Myc-targeting siRNA) in a core–shell lipoplex for
Nanotechnology – a robust tool for fighting the challenges of drug resistance in non-small cell lung cancer
Beilstein J. Nanotechnol. 2023, 14, 240–261, doi:10.3762/bjnano.14.23
Theranostic potential of self-luminescent branched polyethyleneimine-coated superparamagnetic iron oxide nanoparticles
Beilstein J. Nanotechnol. 2022, 13, 82–95, doi:10.3762/bjnano.13.6
- pathways do not necessarily involve traffic to lysosomes, it can yield a better transfection efficiency [65]. Indeed, it was shown that both unmodified branched and linear PEI polyplexes could damage plasma membranes, resulting in a rapid redistribution of phosphatidylserine from the inner plasma membrane
- to the outer cell surface (without activation of caspase 3) and also leakage of cytosolic lactate dehydrogenase (LDH) from the cells [55][56]. It has also been known that unmodified polyplexes can switch on the intrinsic apoptotic pathway by activating the box protein and therefore releasing the
Poly(1-vinylimidazole) polyplexes as novel therapeutic gene carriers for lung cancer therapy
Beilstein J. Nanotechnol. 2020, 11, 354–369, doi:10.3762/bjnano.11.26
- 100, Perkin Elmer, USA). Dynamic light scattering (DLS) and zeta potential measurements: The hydrodynamic size of the PVI–siRNA polyplexes was measured on a Zetasizer Nano ZS (Malvern instruments, UK). Samples at different volume ratios containing a final siRNA concentration of 100 nM were used for
- : The polyplexes with a tracking dye (bromophenol blue, 2 µL), were loaded on a 1% agarose gel. The electrophoresis was carried in Tris/boric acid buffer (TBE) buffer at 80 V for 45 min. The gel was imaged after staining with ethidium bromide in a UV transilluminator using a gel documentation system
- (Fusion SoloX, Vilber Lourmat, France). Heparin displacement assay: Polyplexes were prepared in a volume ratio of 4:1 with a final siRNA concentration of 100 nM and incubated with heparin (low molecular weight fraction, Sigma-Aldrich, USA) solutions of different concentrations for 30 min and expressed as
The different ways to chitosan/hyaluronic acid nanoparticles: templated vs direct complexation. Influence of particle preparation on morphology, cell uptake and silencing efficiency
Beilstein J. Nanotechnol. 2019, 10, 2594–2608, doi:10.3762/bjnano.10.250
- as the cationic component in polyplexes and other drug delivery vehicles [1][2][3]. In comparison to other polycations, its main advantages are the low toxicity and its biodegradability. Biodegradation can occur both enzymatically and oxidatively [4]. A number of methods can be employed to prepare
Development of polycationic amphiphilic cyclodextrin nanoparticles for anticancer drug delivery
Beilstein J. Nanotechnol. 2017, 8, 1457–1468, doi:10.3762/bjnano.8.145
- used for gene delivery studies due to net positive surface charge, facilitating the condensation of negatively charged DNA to form polyplexes [40][41]. In addition, CS-6OCaproβCD nanoparticles are also positively charged due to coating with cationic polymer. It is known that chitosan is a natural
Cationic PEGylated polycaprolactone nanoparticles carrying post-operation docetaxel for glioma treatment
Beilstein J. Nanotechnol. 2017, 8, 1446–1456, doi:10.3762/bjnano.8.144
- particles (which have neutral or near-neutral surface charge) are more prone to escape from the MPS. Cationic nanoparticles can also condense nucleic acid (DNA, RNA) or proteins to form polyplexes for intracellular gene/drug delivery. In this context, chitosan (CS) is used as a positively charged coating
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