Serum heat inactivation diminishes ApoE-mediated uptake of D-Lin-MC3-DMA lipid nanoparticles

• Demian van Straten,
• Luuk van de Schepop,
• Rowan Frunt,
• Pieter Vader and
• Raymond M. Schiffelers

Beilstein J. Nanotechnol. 2025, 16, 740–748, doi:10.3762/bjnano.16.57

• ) ionizable lipids. Cellular uptake and siRNA delivery efficiency of the LNPs were determined in media containing untreated or heat-inactivated serum. Mechanistically, we found that apolipoprotein E, a protein corona component that is crucial for MC3 LNP tropism, displayed reduced stability and functionality

Synthetic-polymer-assisted antisense oligonucleotide delivery: targeted approaches for precision disease treatment

• Ana Cubillo Alvarez,
• Dylan Maguire and
• Ruairí P. Brannigan

Beilstein J. Nanotechnol. 2025, 16, 435–463, doi:10.3762/bjnano.16.34

• reduction in tumour size and metastasis in both orthotopic and xenograft models. Importantly, the PEG-PLO-based delivery system showed a lower nitrogen-to-phosphate ratio requirement compared to similar systems using PEG-PLL, thereby reducing the amount of polymer needed for effective siRNA delivery. The

• cytotoxicity, requiring careful optimisation for therapeutic applications. In their pursuit of more effective gene delivery systems, Kim et al. explored the potential of poly(ʟ-arginine) as a vital component in targeting CD44-positive cancer cells through a novel siRNA delivery platform [94]. The researchers

• cytotoxicity compared to poly(ʟ-arginine) alone, with a 23-fold higher TC50 value observed for HP101. In a similar study, Noh et al. investigated the use of PLR as a key component in a novel siRNA delivery system [95]. PLR was grafted onto chitosan (CS) and further PEGylated to enhance delivery efficiency and

Nanotechnological approaches for efficient N2B delivery: from small-molecule drugs to biopharmaceuticals

• Selin Akpinar Adscheid,
• Akif E. Türeli,
• Nazende Günday-Türeli and
• Marc Schneider

Beilstein J. Nanotechnol. 2024, 15, 1400–1414, doi:10.3762/bjnano.15.113

• irradiation increased the mRNA expression in the cytoplasm [160]. In addition to polyplexes, lipoplexes, another widely used vector, have also been studied for siRNA delivery. For instance, Hu et al. described the encapsulation of siRNA (specifically of c-Myc-targeting siRNA) in a core–shell lipoplex for

Nanotechnology – a robust tool for fighting the challenges of drug resistance in non-small cell lung cancer

• Filip Gorachinov,
• Fatima Mraiche,
• Diala Alhaj Moustafa,
• Ola Hishari,
• Yomna Ismail,
• Jensa Joseph,
• Maja Simonoska Crcarevska,
• Marija Glavas Dodov,
• Nikola Geskovski and
• Katerina Goracinova

Beilstein J. Nanotechnol. 2023, 14, 240–261, doi:10.3762/bjnano.14.23

• , and H1993 cells harbored MET amplification. Therefore, three types of siRNA were used as EGFR–scFv–arginine nonamer peptide fusion protein complexes, namely siRNA for KRAS, EGFR, and MET gene expression silencing (Figure 1). The synergistic effect of gefitinib and scFv antibody-mediated siRNA delivery

• the issue of resistance. Some of the recent approaches in the design of nanocarriers for overcoming the challenges of siRNA delivery and therapeutic nanosystems for combined RNAi treatments of resistant NSCLC will be discussed below. The delivery of siRNA therapy to its targets in vivo is a demanding

• siRNA delivery should generally be positively charged to facilitate siRNA loading by electrostatic interactions. Frequently used traditional polymer materials as nonviral vectors for siRNA encapsulation are polyethyleneimine (PEI), cationic dendrimers, phospholipids, cationic lipids, polysaccharides

Theranostic potential of self-luminescent branched polyethyleneimine-coated superparamagnetic iron oxide nanoparticles

• Rouhollah Khodadust,
• Ozlem Unal and
• Havva Yagci Acar

Beilstein J. Nanotechnol. 2022, 13, 82–95, doi:10.3762/bjnano.13.6

• recently due to a very weak luminescence. Lin et al. synthesized mostly linear PEI and demonstrated a siRNA delivery with this luminescent polymer [27]. Sun et al. utilized the luminescence of branched PEI as the imaging modality in polymeric quantum dots (PDOTs) formed from amphiphilic polyethyleneimine

Brome mosaic virus-like particles as siRNA nanocarriers for biomedical purposes

• Alfredo Nuñez-Rivera,
• Pierrick G. J. Fournier,
• Danna L. Arellano,
• Ana G. Rodriguez-Hernandez,
• Rafael Vazquez-Duhalt and
• Ruben D. Cadena-Nava

Beilstein J. Nanotechnol. 2020, 11, 372–382, doi:10.3762/bjnano.11.28

• chlorotic mottle virus (CCMV); nanocarriers; plant virus-like particles (VLPs); siRNA delivery; small interfering RNA (siRNA); Introduction Despite many efforts taken, the efficient and specific delivery of therapeutic molecules to tumor cells is still a unsolved challenge. Cancer therapies are often

• properties of biomedical interest are demonstrated, such as biocompatibility, tumor cell internalization, and their efficiency as nanocarriers for siRNA delivery. In addition, the capacity of the BMV and CCMV viruses to modulate the immune response in vitro was also analyzed. Results and Discussion Cell

Poly(1-vinylimidazole) polyplexes as novel therapeutic gene carriers for lung cancer therapy

• Gayathri Kandasamy,
• Elena N. Danilovtseva,
• Vadim V. Annenkov and
• Uma Maheswari Krishnan

Beilstein J. Nanotechnol. 2020, 11, 354–369, doi:10.3762/bjnano.11.26

• formation and extension of the endothelial cell processes as observed in the case of the polyplex-treated HUVECs in the present study. The anti-angiogenesis effect of VEGF silencing has been well documented in literature [35]. Our studies concur with these reports and show that the VEGF siRNA delivery

• modulated by the polyplex treatment in A549 cells shows the regulation of a number of genes involved in cancer signaling (Figure 14). PVI-mediated siRNA delivery has a significant influence in the expression of several gene targets involved in cancer cell signaling when compared with free siRNA treatment

Internalization mechanisms of cell-penetrating peptides

• Ivana Ruseska and
• Andreas Zimmer

Beilstein J. Nanotechnol. 2020, 11, 101–123, doi:10.3762/bjnano.11.10

• compartments in the perinuclear region. Andaloussi et al. also developed a stable CPP-siRNA system, derived from transportan-10, containing a proton-acceptor moiety and a stearyl group (for lipophilic interaction), called PepFect6. This system has shown to promote siRNA delivery to difficult-to-transfect cells

The different ways to chitosan/hyaluronic acid nanoparticles: templated vs direct complexation. Influence of particle preparation on morphology, cell uptake and silencing efficiency

• Arianna Gennari,
• Julio M. Rios de la Rosa,
• Erwin Hohn,
• Maria Pelliccia,
• Enrique Lallana,
• Roberto Donno,
• Annalisa Tirella and
• Nicola Tirelli

Beilstein J. Nanotechnol. 2019, 10, 2594–2608, doi:10.3762/bjnano.10.250

• all a higher aspect ratio (Rg/RH) and a lower fractal dimension. We then compared the kinetics of uptake and the (antiluciferase) siRNA delivery performance in murine RAW 264.7 macrophages and in human HCT-116 colorectal tumor cells. The preparative method (and therefore the internal particle

• particles displaying an HA corona are also the least compact, which corroborates the above hypothesis for the corona formation (small compact particles connected by loosely bound HA). Evaluation of CD44-targeted delivery of siRNA One of the most fundamental aspects to a successful intracellular siRNA

• delivery is the ability of the carrier to protect the cargo from enzymatic degradation to allow its release in the cytoplasm, where the RNA machinery is located. Hence, as a first step we ruled out any differences in the protective behaviour of our nanoparticles between preparative methods (Figure 4

Long-term stability and scale-up of noncovalently bound gold nanoparticle-siRNA suspensions

• Anna V. Epanchintseva,
• Julia E. Poletaeva,
• Dmitrii V. Pyshnyi,
• Elena I. Ryabchikova and
• Inna A. Pyshnaya

Beilstein J. Nanotechnol. 2019, 10, 2568–2578, doi:10.3762/bjnano.10.248

• of physicochemical characteristics and siRNA surface density for a long period. Keywords: colloidal stability; gold nanoparticles; scale-up; siRNA delivery; siRNA duplex stability; therapeutic nucleic acids; Introduction Drug delivery to cells is only one application of nanoparticles in biomedicine

Enhanced antineoplastic/therapeutic efficacy using 5-fluorouracil-loaded calcium phosphate nanoparticles

• Shanid Mohiyuddin,
• Saba Naqvi and
• Gopinath Packirisamy

Beilstein J. Nanotechnol. 2018, 9, 2499–2515, doi:10.3762/bjnano.9.233

• nanoparticles in biomedical applications is extended to tissue engineering, gene/siRNA delivery, anticancer drug delivery, protein and antigen delivery, vaccine delivery, insulin as well as imaging probe or contrasting agent delivery for bio-imaging. 5-Fluorouracil (5-FU), a well-known anticancer agent

Hematopoietic and mesenchymal stem cells: polymeric nanoparticle uptake and lineage differentiation

• Ivonne Brüstle,
• Thomas Simmet,
• Gerd Ulrich Nienhaus,
• Katharina Landfester and
• Volker Mailänder

Beilstein J. Nanotechnol. 2015, 6, 383–395, doi:10.3762/bjnano.6.38

• granulopoetic markers was significantly suppressed. Discussion Although nanoparticles are proposed as useful drug and DNA or siRNA delivery vehicles (which could potentially change the fate of stem cells), they could also influence the cellular fate of stem cells with a potentially devastating effect. While