PVP-coated, negatively charged silver nanoparticles: A multi-center study of their physicochemical characteristics, cell culture and in vivo experiments

• Sebastian Ahlberg,
• Alexandra Antonopulos,
• Jörg Diendorf,
• Ralf Dringen,
• Matthias Epple,
• Rebekka Flöck,
• Wolfgang Goedecke,
• Christina Graf,
• Nadine Haberl,
• Jens Helmlinger,
• Fabian Herzog,
• Frederike Heuer,
• Stephanie Hirn,
• Christian Johannes,
• Stefanie Kittler,
• Manfred Köller,
• Katrin Korn,
• Wolfgang G. Kreyling,
• Fritz Krombach,
• Jürgen Lademann,
• Kateryna Loza,
• Eva M. Luther,
• Marcelina Malissek,
• Martina C. Meinke,
• Daniel Nordmeyer,
• Anne Pailliart,
• Jörg Raabe,
• Fiorenza Rancan,
• Barbara Rothen-Rutishauser,
• Eckart Rühl,
• Carsten Schleh,
• Andreas Seibel,
• Christina Sengstock,
• Lennart Treuel,
• Annika Vogt,
• Katrin Weber and
• Reinhard Zellner

Beilstein J. Nanotechnol. 2014, 5, 1944–1965, doi:10.3762/bjnano.5.205

Different endocytotic uptake mechanisms for nanoparticles in epithelial cells and macrophages

• Dagmar A. Kuhn,
• Dimitri Vanhecke,
• Benjamin Michen,
• Fabian Blank,
• Peter Gehr,
• Alke Petri-Fink and
• Barbara Rothen-Rutishauser

Beilstein J. Nanotechnol. 2014, 5, 1625–1636, doi:10.3762/bjnano.5.174

• caveolin-1. A549 cells expressed clathrin heavy chain and caveolin-1, but no flotillin-1 uptake-related proteins. Our data revealed an impeded uptake of 40 nm polystyrene nanoparticles by J774A.1 macrophages when actin polymerization and clathrin-coated pit formation was blocked. From this result, it is

• suggested that macropinocytosis and phagocytosis, as well as clathrin-mediated endocytosis, play a crucial role. The uptake of 40 nm nanoparticles in alveolar epithelial A549 cells was inhibited after depletion of cholesterol in the plasma membrane (preventing caveolin-mediated endocytosis) and inhibition

• detected. Clathrin heavy chain was detected within the cells, both at the cell membrane and in the cytosol. Flotillin-1 was, however, only observed in the cytosol. In A549 cells, clathrin heavy chain and caveolin-1 were located in the cells both at the cell membrane as well as in the cytoplasm, albeit to a

Silica nanoparticles are less toxic to human lung cells when deposited at the air–liquid interface compared to conventional submerged exposure

• Alicja Panas,
• Andreas Comouth,
• Harald Saathoff,
• Thomas Leisner,
• Marco Al-Rawi,
• Michael Simon,
• Gunnar Seemann,
• Olaf Dössel,
• Sonja Mülhopt,
• Hanns-Rudolf Paur,
• Susanne Fritsch-Decker,
• Carsten Weiss and
• Silvia Diabaté

Beilstein J. Nanotechnol. 2014, 5, 1590–1602, doi:10.3762/bjnano.5.171

• deposition efficiencies of up to 100% for charged particles [11]. de Bruijne et al. [21] used a corona charger for efficient charging of aerosol particles and did not observe adverse effects on A549 cells by the trace gases formed in the corona. However, for particle sizes below 50 nm, the probability to be

• fluorescence analyses. Industrial SiO2 NPs (Aerosil®200, Evonik) produced by flame synthesis and SiO2 NPs produced by the Stöber method (Postnova Analytics, Landsberg) were used to test the biological responses in A549 cells with and without an electrostatic field at the ALI and under submerged conditions

• calculated extremes and is given as (52 ± 26) µg·cm−2. Biological effects For the determination of biological effects the Transwell inserts covered with a confluent layer of A549 cells were exposed to filtered and unfiltered aerosol. Exposure to the unfiltered aerosol of Aerosil200 NP and SiO2-50 nm NP

Mimicking exposures to acute and lifetime concentrations of inhaled silver nanoparticles by two different in vitro approaches

• Fabian Herzog,
• Kateryna Loza,
• Sandor Balog,
• Martin J. D. Clift,
• Matthias Epple,
• Peter Gehr,
• Alke Petri-Fink and
• Barbara Rothen-Rutishauser

Beilstein J. Nanotechnol. 2014, 5, 1357–1370, doi:10.3762/bjnano.5.149

• . Ag NP exposure and dose determination Triple cell co-cultures composed of A549 cells, MDMs, and MDDCs [40][42] were exposed at the ALI, similarly as described in [44], to three different concentrations of Ag NPs with a final areal density of 0.03, 0.3, and 3 µg Ag/cm2. Furthermore, cells were exposed

• culture Experiments were carried out with a triple cell co-culture model of the human epithelial alveolar/airway barrier as described in detail by [40][61][67]. Briefly, A549 cells (adenocarcinomic human derived alveolar type II epithelial cells) were cultivated in Roswell Park Memorial Institute (RPMI