Involvement of two uptake mechanisms of gold and iron oxide nanoparticles in a co-exposure scenario using mouse macrophages

• Dimitri Vanhecke,
• Dagmar A. Kuhn,
• Dorleta Jimenez de Aberasturi,
• Sandor Balog,
• Ana Milosevic,
• Dominic Urban,
• Diana Peckys,
• Niels de Jonge,
• Wolfgang J. Parak,
• Alke Petri-Fink and
• Barbara Rothen-Rutishauser

Beilstein J. Nanotechnol. 2017, 8, 2396–2409, doi:10.3762/bjnano.8.239

• mode was found (Figure 6). A number of convoluted factors influenced the average fluorescence in a cell: The cell cycle influences the uptake rate and after mitosis (population doubling time: about 17 h) the NP load is split between the two daughter cells [30]. Furthermore, the uptake of NPs requires

A nanocomplex of C₆₀ fullerene with cisplatin: design, characterization and toxicity

• Svitlana Prylutska,
• Svitlana Politenkova,
• Kateryna Afanasieva,
• Volodymyr Korolovych,
• Kateryna Bogutska,
• Andriy Sivolob,
• Larysa Skivka,
• Maxim Evstigneev,
• Viktor Kostjukov,
• Yuriy Prylutskyy and
• Uwe Ritter

Beilstein J. Nanotechnol. 2017, 8, 1494–1501, doi:10.3762/bjnano.8.149

• are transformed into lymphoblast) a large set of genes are activated to allow the entry of cells in the G1 phase of the cell cycle [51]. Probably, such transformation that never occurs in vivo in lymphocytes under normal conditions, leads to an increase in the cells' sensitivity to the anticancer drug

Development of polycationic amphiphilic cyclodextrin nanoparticles for anticancer drug delivery

• Gamze Varan,
• Juan M. Benito,
• Carmen Ortiz Mellet and
• Erem Bilensoy

Beilstein J. Nanotechnol. 2017, 8, 1457–1468, doi:10.3762/bjnano.8.145

• differences of CD nanoparticles may be related with drug release profiles. PCX shows anticancer activity by stabilizing microtubules and blocking the cell in G2 or M phase in cell cycle [55][56]. The duration of drug release of PCX-loaded amphiphilic CD nanoparticles increases in the order of 6OCaproβCD < CS

Carbon nanomaterials sensitize prostate cancer cells to docetaxel and mitomycin C via induction of apoptosis and inhibition of proliferation

• Kati Erdmann,
• Jessica Ringel,
• Silke Hampel,
• Manfred P. Wirth and
• Susanne Fuessel

Beilstein J. Nanotechnol. 2017, 8, 1307–1317, doi:10.3762/bjnano.8.132

• cell-cycle arrest and apoptosis via DNA crosslinking. The present study investigated the influence of CNFs and CNTs co-exposed with DTX and MMC on cellular function of PCa cells in comparison to the individual effects. Results Effect on cellular viability First, the influence of carbon nanomaterials

Nano-engineered skin mesenchymal stem cells: potential vehicles for tumour-targeted quantum-dot delivery

• Liga Saulite,
• Dominyka Dapkute,
• Karlis Pleiko,
• Ineta Popena,
• Simona Steponkiene,
• Ricardas Rotomskis and
• Una Riekstina

Beilstein J. Nanotechnol. 2017, 8, 1218–1230, doi:10.3762/bjnano.8.123

• affect the osteogenesis and chondrogenesis capacities of bone marrow MSCs [17]. The impact of QD labelling on the biological properties of targeted stem cells, such as proliferation, cell cycle, and apoptosis, remains elusive. Therefore, further research on MSCs with regard to the delivery of QDs for

• overnight. The medium was subsequently aspirated, and the wells were rinsed once with serum-free medium. The cells were serum-starved for 24 h to synchronize the cell cycle. Next, 16 nM of QDs in complete medium were added, and the cells were incubated for 24 or 48 h. Control wells contained cells in

Association of aescin with β- and γ-cyclodextrins studied by DFT calculations and spectroscopic methods

• Ana I. Ramos,
• Pedro D. Vaz,
• Susana S. Braga and
• Artur M. S. Silva

Beilstein J. Nanotechnol. 2017, 8, 348–357, doi:10.3762/bjnano.8.37

• vivo using xenograft mice, showed cytotoxic effects for aescin, through the induction of apoptosis and G2/M cell cycle arrest [9]. Currently, aescin is mostly employed for venotonic action, being available in the form of topical formulations such as lotions, gels and creams. Many of these products

Nano- and microstructured materials for in vitro studies of the physiology of vascular cells

• Alexandra M. Greiner,
• Adria Sales,
• Hao Chen,
• Sarah A. Biela,
• Dieter Kaufmann and
• Ralf Kemkemer

Beilstein J. Nanotechnol. 2016, 7, 1620–1641, doi:10.3762/bjnano.7.155

• proliferation rate [277]. ECs: Differently regulated genes from cells on microstructures, in comparison to cells on flat surfaces, showed that endothelial cells on microgrooves down-regulated genes related to the cell cycle as well as their gene for β1 integrin [278]. In the future, it would be interesting to

Unraveling the neurotoxicity of titanium dioxide nanoparticles: focusing on molecular mechanisms

• Bin Song,
• Yanli Zhang,
• Jia Liu,
• Xiaoli Feng,
• Ting Zhou and
• Longquan Shao

Beilstein J. Nanotechnol. 2016, 7, 645–654, doi:10.3762/bjnano.7.57

• also internalized by C6 and U373 cells [52]. Valdiglesias et al. [53] found that NPs were internalized by SH-SY5Y neuronal cells exposed to TiO2 NPs, which coincided with alterations in the cell cycle and an elevation in the proportion of apoptotic cells. Damage of the DNA was induced and NO oxidative

• disrupted cell cycle, leading to apoptosis and excessive ROS [63]. Uncertain mechanisms underlying TiO2 NPs-induced neurotoxicity Autophagy Autophagy can be divided into three types, 1) microautophagy, 2) macroautophagy, and 3) identified-chaperone-mediated autophagy. It is believed that macroautophagy

Application of biclustering of gene expression data and gene set enrichment analysis methods to identify potentially disease causing nanomaterials

• Andrew Williams and
• Sabina Halappanavar

Beilstein J. Nanotechnol. 2015, 6, 2438–2448, doi:10.3762/bjnano.6.252

• black (CB) or carbon nanotubes (CNTs) to determine the disease significance of these data-driven gene sets. Results: Biclusters representing inflammation (chemokine activity), DNA binding, cell cycle, apoptosis, reactive oxygen species (ROS) and fibrosis processes were identified. All of the NM studies

• Genomes (KEGG) pathways were found significant (FDR p-value < 0.05). Ten of the twenty gene symbols from this bicluster were elements of the cell cycle GO (FDR p-value = 4.9 × 10−6) and five were part of the KEGG pathway (FDR p-value = 2.5 × 10−4). The bleomycin injury and the bacterial infection models

• potentially fibrogenic NMs. Although several genes sets associated with acute DNA binding, cell cycle, apoptosis, and ROS response that were specific to different disease models were also observed to be perturbed following exposure to NMs, the implication of such perturbation was not clear from this analysis

PLGA nanoparticles as a platform for vitamin D-based cancer therapy

• Maria J. Ramalho,
• Joana A. Loureiro,
• Bárbara Gomes,
• Manuela F. Frasco,
• Manuel A. N. Coelho and
• M. Carmo Pereira

Beilstein J. Nanotechnol. 2015, 6, 1306–1318, doi:10.3762/bjnano.6.135

• to the free calcitriol results. At this concentration the inhibitory effect on nontumor cells (hTERT-HPNE) decreased to 65%. This study highlights the ability of PLGA nanoparticles to deliver vitamin D3 into cancer cells, with major effects regarding cancer cell cycle arrest and major changes in the

• cell growth, cell cycle arrest and morphological changes. Results Nanoparticle physicochemical properties PLGA NPs were prepared by a single emulsion solvent evaporation method and stabilized with Pluronic®F127. The obtained results for mean the diameter, polydispersity index (PDI) and zeta potential

• form of calcitriol. This was the cell line where the encapsulation of calcitriol in NPs proved to be more advantageous. Cell cycle arrest by calcitriol-loaded PLGA NPs To assess whether the cytotoxic effects of calcitriol are due to cell cycle inhibition, cell cycle analysis by flow cytometry was

Nanoparticle interactions with live cells: Quantitative fluorescence microscopy of nanoparticle size effects

• Li Shang,
• Karin Nienhaus,
• Xiue Jiang,
• Linxiao Yang,
• Katharina Landfester,
• Volker Mailänder,
• Thomas Simmet and
• G. Ulrich Nienhaus

Beilstein J. Nanotechnol. 2014, 5, 2388–2397, doi:10.3762/bjnano.5.248

• (RBCs) can internalize NPs only via passive transport [12]. The efficiency of NP internalization by a cell depends on cell-specific parameters such as cell type or cell cycle phase [13][14] and physicochemical properties of the NP [15]. Notably, NPs with positive surface charge are typically

Interaction of dermatologically relevant nanoparticles with skin cells and skin

• Annika Vogt,
• Fiorenza Rancan,
• Sebastian Ahlberg,
• Berouz Nazemi,
• Chun Sik Choe,
• Maxim E. Darvin,
• Sabrina Hadam,
• Ulrike Blume-Peytavi,
• Kateryna Loza,
• Jörg Diendorf,
• Matthias Epple,
• Christina Graf,
• Eckart Rühl,
• Martina C. Meinke and
• Jürgen Lademann

Beilstein J. Nanotechnol. 2014, 5, 2363–2373, doi:10.3762/bjnano.5.245

• interactions between proteins in media may occur in a concentration-dependent manner and influence the particle–cell interactions, which is supported by our previous findings on the different aggregation behaviors of particles in biological media. Changes in the release of inflammatory cytokines and cell cycle

Precise quantification of silica and ceria nanoparticle uptake revealed by 3D fluorescence microscopy

• Adriano A. Torrano and
• Christoph Bräuchle

Beilstein J. Nanotechnol. 2014, 5, 1616–1624, doi:10.3762/bjnano.5.173

• ., cell-cycle phase dependency, and load capacity), and the number of nanoparticles available for uptake. Conclusion The possibility to quantify nanoparticles on the single-cell level is an important step to better understand the mechanisms of nanoparticles-cell interactions. In this work it was

The softening of human bladder cancer cells happens at an early stage of the malignancy process

• Jorge R. Ramos,
• Joanna Pabijan,
• Ricardo Garcia and
• Malgorzata Lekka

Beilstein J. Nanotechnol. 2014, 5, 447–457, doi:10.3762/bjnano.5.52

• their density. The elastic properties of cancerous cells (HTB-9, HT-1376 and T24) were independent of the cell line, the stage of cancer progression, and the cell cycle. Therefore, they underline the usefulness of atomic force microscopy to detect bladder cancer cells because regardless on the state of