Hematopoietic and mesenchymal stem cells: polymeric nanoparticle uptake and lineage differentiation

• Ivonne Brüstle,
• Thomas Simmet,
• Gerd Ulrich Nienhaus,
• Katharina Landfester and
• Volker Mailänder

Beilstein J. Nanotechnol. 2015, 6, 383–395, doi:10.3762/bjnano.6.38

• (magnetite) embedded (PLLA–Fe). Confocal laser scanning microscopy (cLSM) was performed to determine the intracellular localization of the nanoparticles. The images in Figure 2 clearly show that all particle types were indeed internalized by the cells and not only attached to the cell surface. hHSCs showed a

• , apoptotic, dead) and taking the percentage of gated cells in the respective gate. Signals from APC-coupled antibodies were analyzed by overlays of several signals in die APC channel. Confocal laser scanning microscopy (cLSM) Confocal laser scanning microscopy was performed to validate the intracellular

Imaging the intracellular degradation of biodegradable polymer nanoparticles

• Anne-Kathrin Barthel,
• Martin Dass,
• Melanie Dröge,
• Jens-Michael Cramer,
• Daniela Baumann,
• Markus Urban,
• Katharina Landfester,
• Volker Mailänder and
• Ingo Lieberwirth

Beilstein J. Nanotechnol. 2014, 5, 1905–1917, doi:10.3762/bjnano.5.201

• period of 14 days, primarily by means of transmission electron microscopy (TEM), in order to demonstrate their degradation. Furthermore, confocal laser scanning microscopy (CLSM) and flow cytometry were used to monitor the nanoparticle load of individual cells. As a probe we chose tailor-made PLLA

• cultures. All values are triplicates with the error bars representing the standard deviation. Confocal laser scanning microscopy (CLSM) Confocal laser scanning microscopy (CLSM) was applied to demonstrate the intracellular distribution of nanoparticles over the period of 14 days. As described in [26], for

• confocal laser scanning microscopy (CLSM), MSCs were seeded in α-MEM solution at a density of 20 000 cells in ibiTreat µ-slides (IBIDI, Germany). On the following day particles were added to the medium at a concentration of 300 μg·mL−1. After an incubation time of 24 h, the supernatant was removed and

Dry friction of microstructured polymer surfaces inspired by snake skin

• Martina J. Baum,
• Lars Heepe,
• Elena Fadeeva and
• Stanislav N. Gorb

Beilstein J. Nanotechnol. 2014, 5, 1091–1103, doi:10.3762/bjnano.5.122

• elevated, so the snake can generate propulsion due to the interlocking of its microstructure with surface asperities. The results of the study of the snake skin’s microstructure by using atomic force microscopy (AFM) and confocal laser scanning microscopy (CLSM) showed that the anisotropic geometry of the

Fibrillar adhesion with no clusterisation: Functional significance of material gradient along adhesive setae of insects

• Stanislav N. Gorb and
• Alexander E. Filippov

Beilstein J. Nanotechnol. 2014, 5, 837–845, doi:10.3762/bjnano.5.95

• revealed by confocal laser scanning microscopy (CLSM). This gradient is hypothesized to be an evolutionary optimization enhancing adaptation of adhesive pads to rough surfaces, while simultaneously preventing setal clusterisation. Such an optimisation presumably increases the performance of the adhesive