Hematopoietic and mesenchymal stem cells: polymeric nanoparticle uptake and lineage differentiation

• Ivonne Brüstle,
• Thomas Simmet,
• Gerd Ulrich Nienhaus,
• Katharina Landfester and
• Volker Mailänder

Beilstein J. Nanotechnol. 2015, 6, 383–395, doi:10.3762/bjnano.6.38

• (Invitrogen), and excited with 543 nm laser light and the emission was collected at 565–585 nm. To avoid crosstalk between the channels, emission signals were collected independently in serial mode. Induction of differentiation for hHSCs In vitro differentiation of hHSCs was performed as previously described

Nanoparticle interactions with live cells: Quantitative fluorescence microscopy of nanoparticle size effects

• Li Shang,
• Karin Nienhaus,
• Xiue Jiang,
• Linxiao Yang,
• Katharina Landfester,
• Volker Mailänder,
• Thomas Simmet and
• G. Ulrich Nienhaus

Beilstein J. Nanotechnol. 2014, 5, 2388–2397, doi:10.3762/bjnano.5.248

• filter (AHF, Tübingen, Germany). Detailed information on the experimental conditions is given in Table 3. Suitable control experiments were performed to ensure negligible crosstalk between the two channels. Images were quantitatively analyzed using the software ImageJ [51]. The fluorescence intensity of

Uncertainties in forces extracted from non-contact atomic force microscopy measurements by fitting of long-range background forces

• Adam Sweetman and
• Andrew Stannard

Beilstein J. Nanotechnol. 2014, 5, 386–393, doi:10.3762/bjnano.5.45

• oscillation amplitudes (A0) of between 0.1 and 0.3 nm during NC-AFM imaging. In order to eliminate any possible effect from either electronic crosstalk [16] or the so-called “Phantom Force” [17] all NC-AFM imaging was performed at 0 V (i.e., no detectable tunnel current). To stabilise the imaging conditions a

Influence of the adsorption geometry of PTCDA on Ag(111) on the tip–molecule forces in non-contact atomic force microscopy

• Gernot Langewisch,
• Jens Falter,
• André Schirmeisen and
• Harald Fuchs

Beilstein J. Nanotechnol. 2014, 5, 98–104, doi:10.3762/bjnano.5.9

• oscillation was held constant at A = 0.40 nm. To avoid crosstalk between tunneling current and deflection signal, no voltage was applied to the tip during NC-AFM operation. The tip was prepared by voltage pulses and soft indentation into the Ag sample most likely resulting in a Ag-terminated apex. However, it

Noise performance of frequency modulation Kelvin force microscopy

• Heinrich Diesinger,
• Dominique Deresmes and
• Thierry Mélin

Beilstein J. Nanotechnol. 2014, 5, 1–18, doi:10.3762/bjnano.5.1

• proposed that ensures making best use of the available PLL bandwidth with negligible crosstalk between the loops and that yields equal bandwidth for both loops. The Kelvin output noise reduces to a compact analytic expression in terms of probe merit factors and a criterion for the transition between

• voltage noise PSD”), and on the other hand, the overlap and hence crosstalk between topography and KFM image is small since the roll-off of the distance controller at 1 BW and of the lower PLL frequency satellite of the Kelvin controller at fmod − fc,KFM = 3 BW are 2 BW apart. The cutoff frequency of the

• CPD represented by Vpert varying at fc = BW causes a response of the distance controller at 3fc at −24 dB below its response to a static CPD. According to Equation 24, an AC CPD represented by Vpert, as well as the AC voltage Vmod, also both introduce a static term. The crosstalk onto the distance

High-resolution nanomechanical analysis of suspended electrospun silk fibers with the torsional harmonic atomic force microscope

• Mark Cronin-Golomb and
• Ozgur Sahin

Beilstein J. Nanotechnol. 2013, 4, 243–248, doi:10.3762/bjnano.4.25

• crosstalk from large vertical signals into torsional vibration signals. Once the tip–sample-force waveform is determined, the program constructs force–distance curves using the distance information in the vertical deflection signals [20]. It is possible to analyze these force–distance curves according to

Spring constant of a tuning-fork sensor for dynamic force microscopy

• Dennis van Vörden,
• Manfred Lange,
• Merlin Schmuck,
• Nico Schmidt and
• Rolf Möller

Beilstein J. Nanotechnol. 2012, 3, 809–816, doi:10.3762/bjnano.3.90

• evaluation of the spring constant was performed for a TF with a tip (e.g., a 25–100 μm tungsten wire) attached to the front face of the free prong of the tuning fork. It is connected separately through a metallic wire (e.g., a 25 μm gold wire) to collect the tunneling current, avoiding crosstalk with the

Repulsive bimodal atomic force microscopy on polymers

• Alexander M. Gigler,
• Christian Dietz,
• Maximilian Baumann,
• Nicolás F. Martinez,
• Ricardo García and
• Robert W. Stark

Beilstein J. Nanotechnol. 2012, 3, 456–463, doi:10.3762/bjnano.3.52

• tip and the sample occurred. Such a stabilization of the repulsive regime has been previously observed [27]. During closer approaches, the amplitude of the higher eigenmode is only slightly influenced despite increasing interactions. Crosstalk between the two modes occurred for a z-distance of

• a net repulsive region in Δ except for the curve with a second-mode free amplitude of A02 = 1 nm. Interestingly, in that case, only the second-eigenmode oscillation makes the transition to a net repulsive force. No crosstalk was found during the entire approach for all amplitude ratios. The results

• , however, was strongly influenced by the oscillation of the fundamental eigenmode. Nevertheless, a high amplitude setpoint ratio A1/A01 for topographical feedback should be chosen; otherwise, crosstalk can occur. Imaging with small amplitude ratios A01/A02 on stiff samples (e.g., silicon) is not stable due

Simultaneous current, force and dissipation measurements on the Si(111) 7×7 surface with an optimized qPlus AFM/STM technique

• Zsolt Majzik,
• Martin Setvín,
• Andreas Bettac,
• Albrecht Feltz,
• Vladimír Cháb and
• Pavel Jelínek

Beilstein J. Nanotechnol. 2012, 3, 249–259, doi:10.3762/bjnano.3.28

• (Figure 4). To demonstrate how the crosstalk affects the deflection signal, we analyzed the configuration shown in Figure 4. To simplify the electric circuit, identical amplifiers were used in both channels (Figure 2). For the given resonant frequency f0 = 73180 Hz the amplifier operates in the charge

• potential oscillation . The magnitude of depends also on the frequency f0, the oscillation amplitude A and the characteristic decay length of the tunneling current κI. Therefore the crosstalk can be enhanced when the sensor is operated at high frequencies. Figure 4 shows that the signal in the deflection

• channel appears significantly altered at the output, VD, for Cs values exceeding 1 pF. In the case of a stray capacitance of 5 pF the crosstalk causes a decrease of the initial value of VD from 273 μV to 250 μV with a 6.8° phase shift. Note here, that by inverting the sign of the bias voltage the result

A measurement of the hysteresis loop in force-spectroscopy curves using a tuning-fork atomic force microscope

• Manfred Lange,
• Dennis van Vörden and
• Rolf Möller

Beilstein J. Nanotechnol. 2012, 3, 207–212, doi:10.3762/bjnano.3.23

• under UHV conditions are about 28 kHz and 10000, respectively. The tuning fork and tunneling signal are wired separately to avoid any crosstalk. Both signals are amplified by home-built current-to-voltage converters outside the vacuum system. For the detection and regulation of the tuning-fork

Effect of the tip state during qPlus noncontact atomic force microscopy of Si(100) at 5 K: Probing the probe

• Adam Sweetman,
• Sam Jarvis,
• Rosanna Danza and
• Philip Moriarty

Beilstein J. Nanotechnol. 2012, 3, 25–32, doi:10.3762/bjnano.3.3

• presented were taken at close to zero bias (i.e., ~0 V applied to the tip, sample held at system ground), in order to eliminate the possibility of electronic crosstalk (Supporting Information File 1) and the effect of tunnelling electrons [19]. To ensure that this was the case, the tunnel current was

Determination of object position, vortex shedding frequency and flow velocity using artificial lateral line canals

• Adrian Klein and
• Horst Bleckmann

Beilstein J. Nanotechnol. 2011, 2, 276–283, doi:10.3762/bjnano.2.32

• ). Its slope (m) is larger than 1. This could be due to vibrations or a crosstalk between adjacent ANs. Scheme of an artificial CN. The drawing is not to scale. Acknowledgements This paper is dedicated to the memory of Joseph Humphrey, a brilliant scientist, friend and exceptional human being. We thank