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Search for "cytokines" in Full Text gives 34 result(s) in Beilstein Journal of Nanotechnology.
Interaction of dermatologically relevant nanoparticles with skin cells and skin
Beilstein J. Nanotechnol. 2014, 5, 2363–2373, doi:10.3762/bjnano.5.245
- interactions between proteins in media may occur in a concentration-dependent manner and influence the particle–cell interactions, which is supported by our previous findings on the different aggregation behaviors of particles in biological media. Changes in the release of inflammatory cytokines and cell cycle
Anticancer efficacy of a supramolecular complex of a 2-diethylaminoethyl–dextran–MMA graft copolymer and paclitaxel used as an artificial enzyme
Beilstein J. Nanotechnol. 2014, 5, 2293–2307, doi:10.3762/bjnano.5.238
- /RISK” of high polymer DDMC/PTX [49]. It is known that the TLR3/TICAM-1 pathway of M1 macrophages in a tumor is the key to TNF-α production [50]. PTX reportedly affects the native or acquired immune response, thereby inducing M1 macrophages, which secrete NO and proinflammatory cytokines such as IL-1β
- enzyme, a very small amount of proinflammatory cytokines must be released, leading to M2 macrophage differentiation. It seems that it is not very rare to receive useless inflammatory signals in an enzyme reaction model. From another point of view, it is imagined that the production of M2 macrophages by
PVP-coated, negatively charged silver nanoparticles: A multi-center study of their physicochemical characteristics, cell culture and in vivo experiments
Beilstein J. Nanotechnol. 2014, 5, 1944–1965, doi:10.3762/bjnano.5.205
- , i.e., oxidative stress and different pro-inflammatory cytokines/chemokines, were observed. Compared to a representative lung deposition attributable to occupational exposure of 5–100 nm silver nanoparticles calculated after 24 h, as described by Gangwal et al. [131], an expected deposited dose in
Biocompatibility of cerium dioxide and silicon dioxide nanoparticles with endothelial cells
Beilstein J. Nanotechnol. 2014, 5, 1795–1807, doi:10.3762/bjnano.5.190
- -inflammatory and pro-thrombotic impact of CeO2 nanoparticles and intracellular ROS generation after CeO2 nanoparticle exposure In order to identify the pro-inflammatory impact of CeO2 nanoparticles, the release of three different cytokines (monocyte chemoattractant protein-1 (MCP-1), interleukin 6 (IL-6), and
- was used (0.2% FBS), since the serum itself could contain cytokines. After the corresponding incubation time, the cell culture supernatants were collected and stored at −80 °C until the human enzyme-linked immunosorbent assays (ELISA) were performed using commercially available kits addressing MCP-1
In vitro and in vivo interactions of selected nanoparticles with rodent serum proteins and their consequences in biokinetics
Beilstein J. Nanotechnol. 2014, 5, 1699–1711, doi:10.3762/bjnano.5.180
- twenty-four hours the viability of the PCLS was tested by lactate dehydrogenase (LDH) analysis for cell membrane damage and a WST-1 assay for mitochondrial activity in the cell culture supernatant as well as the release of the pro-inflammatory cytokines tumor necrosis factor alpha (TNF-α) and interleukin
- -inflammatory cytokines of PCLS exposed to either AgNP or dissolved Ag or mixtures of both. The LDH release of PCLS increased significantly when the rats were instilled with the aqueous supernatants of AgNP centrifugation compared to PCLS of untreated control rats (Figure 7). Consistently, LDH was also
- /digested intracellularly probably faster than the engulfed AgNP that formed Ag+ ions or other reactive Ag species, which apparently caused the increased LDH release and the release of both cytokines [9][27]. Interestingly, instilled silver acetate showed a similar trend as the suspension of the six-month
Silica nanoparticles are less toxic to human lung cells when deposited at the air–liquid interface compared to conventional submerged exposure
Beilstein J. Nanotechnol. 2014, 5, 1590–1602, doi:10.3762/bjnano.5.171
- NPs. The medium was also analysed for the release of the pro-inflammatory cytokines IL-8 and IL-6. Figure 8 shows, that the electrical field and the exposure to clean air had no effect on IL-8 release. The results at the ALI were qualitatively comparable with those obtained under submerged conditions
- without HEPES at 37 °C and 95% humidity and analysed after 24 h. Post-incubation was performed submerged in order to allow optimal release of cytokines into the apical compartment. For comparison, cells grown in Transwell inserts were simultaneously treated under submerged conditions in serum-free RPMI
Mimicking exposures to acute and lifetime concentrations of inhaled silver nanoparticles by two different in vitro approaches
Beilstein J. Nanotechnol. 2014, 5, 1357–1370, doi:10.3762/bjnano.5.149
- . As a release of cytokines could not be detected with ELISA we assume that this is a transient and acute effect, which decreases to normal levels within a short time. Dependent on how NPs are applied, i.e., either by submerged or ALI conditions, different toxicological results can be obtained [60
- proliferation and migration (chemotaxis) both decreased, and the release of cytokines was affected. Increased IL-8 and decreased IL-6 and vascular endothelial growth factor (VEGF) levels were detected at high Ag NP concentrations [65]. These studies however, were obtained with human mesenchymal stem cells
Biocalcite, a multifunctional inorganic polymer: Building block for calcareous sponge spicules and bioseed for the synthesis of calcium phosphate-based bone
Beilstein J. Nanotechnol. 2014, 5, 610–621, doi:10.3762/bjnano.5.72
- HA and HA resorption by osteoclasts and osteoblasts in bone tissue are controlled by a network of cytokines and growth factors. The receptor activator of NF-KB (RANK) and its ligand, receptor activator of NF-KB ligand (RANKL) and osteoprotegerin (OPG) play a key function in regulation of bone
- scaffold must possess the inorganic/organic 3D structure of bone and an appropriate porosity [69] that allows the ingrowth of cells and an efficient transport of cytokines, growth factors, and nutrients. To avoid necrosis within larger implants, a suitable scaffold must also allow an efficient
Cytotoxic and proinflammatory effects of PVP-coated silver nanoparticles after intratracheal instillation in rats
Beilstein J. Nanotechnol. 2013, 4, 933–940, doi:10.3762/bjnano.4.105
- neutrophil numbers (60-fold) of BALF. Furthermore, while there was no change in BALF cytokine levels after the instillation of 50 µg PVP-AgNP, instillation of 250 µg PVP-AgNP resulted in significantly increased levels of seven out of eleven measured cytokines. These finding suggest that exposure to inhaled
- protein levels (Figure 5). Cytokine levels in BALF To assess the proinflammatory effects of PVP-AgNP, we determined the BALF levels of several cytokines and chemokines after intratracheal instillation. Macrophage activators such as IL-1α, IL-1β, IL-6, and IL-12p70 act as proinflammatory cytokines. Figure
- 6A shows that the instillation of 250 µg PVP-AgNP caused an increase in the BALF levels of all four cytokines as compared to controls that was significant for IL-1β, IL-6, and IL-12p70. TNF-α, another proinflammatory cytokine, was not detectable in BALF. Similarly, significantly elevated levels of
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